Previous data suggest that type 1 diabetes mellitus leads to the deterioration of myocardial intercellular communication mediated by connexin-43 (Cx43) channels. We therefore aimed to explore Cx43, PKC signaling and ultrastructure in non-treated and omega-3 fatty acid (omega-3) treated spontaneously diabetic Goto-Kakizaki (GK) rats considered as type 2 diabetes model. Four-week-old GK and non-diabetic Wistar-Clea rats were fed omega-3 (200 mg/kg/day) for 2 months and compared with untreated rats. Real-time PCR and immunoblotting were performed to determine Cx43, PKC-epsilon and PKC-delta expression. In situ Cx43 was examined by immunohistochemistry and subcellular alterations by electron microscopy. Omega-3 intake reduced blood glucose, triglycerides, and cholesterol in diabetic rats and this was associated with improved integrity of cardiomyocytes and capillaries in the heart. Myocardial Cx43 mRNA and protein levels were higher in diabetic versus non-diabetic rats and were further enhanced by omega-3. The ratio of phosphorylated (functional) to non-phosphorylated Cx43 was lower in diabetic compared to non-diabetic rats but was increased by omega-3, in part due to up-regulation of PKC-epsilon. In addition, pro-apoptotic PKC-delta expression was decreased. In conclusion, spontaneously diabetic rats at an early stage of disease benefit from omega-3 intake due to its hypoglycemic effect, upregulation of myocardial Cx43, and preservation of cardiovascular ultrastructure. These findings indicates that supplementation of omega-3 may be beneficial also in the management of diabetes in humans.
- MeSH
- experimentální diabetes mellitus metabolismus patologie prevence a kontrola MeSH
- fixní kombinace léků MeSH
- konexin 43 metabolismus MeSH
- krysa rodu rattus MeSH
- kyselina eikosapentaenová farmakologie terapeutické užití MeSH
- kyseliny dokosahexaenové farmakologie terapeutické užití MeSH
- myokard metabolismus ultrastruktura MeSH
- potravní doplňky MeSH
- preklinické hodnocení léčiv MeSH
- proteinkinasa C-delta metabolismus MeSH
- proteinkinasa C-epsilon metabolismus MeSH
- srdce účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
We examined the protective effect of radon inhalation on streptozotocin (STZ)-induced type-1 diabetes in mice. Mice inhaled radon at concentrations of 1000, 2500, and 5500 Bq/m3 for 24 hours before STZ administration. STZ administration induced characteristics of type-1 diabetes such as hyperglycemia and hypoinsulinemia; however, radon inhalation at doses of 1000 and 5500 Bq/m3 significantly suppressed the elevation of blood glucose in diabetic mice. Serum insulin was significantly higher in mice pre-treated with radon at a dose of 1000 Bq/m3 than in mice treated with a sham. In addition, superoxide dismutase activities and total glutathione contents were significantly higher and lipid peroxide was significantly lower in mice pre-treated with radon at doses of 1000 and 5500 Bq/m3 than in mice treated with a sham. These results were consistent with the result that radon inhalation at 1000 and 5500 Bq/m3 suppressed hyperglycemia. These findings suggested that radon inhalation suppressed STZ-induced type-1 diabetes through the enhancement of antioxidative functions in the pancreas.
- MeSH
- antioxidancia aplikace a dávkování MeSH
- aplikace inhalační MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- diabetes mellitus 1. typu krev chemicky indukované patologie prevence a kontrola MeSH
- experimentální diabetes mellitus krev chemicky indukované patologie prevence a kontrola MeSH
- glutathion metabolismus MeSH
- hypoglykemika aplikace a dávkování MeSH
- inzulin krev MeSH
- krevní glukóza účinky léků metabolismus MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- pankreas účinky léků metabolismus patologie MeSH
- plyny MeSH
- radon aplikace a dávkování MeSH
- streptozocin * MeSH
- superoxiddismutasa metabolismus MeSH
- tělesná hmotnost účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
A series of copper(II) and zinc(II) complexes involving a tridentate O,N,O'-donor Schiff base derived from salicylaldehyde and beta-alanine {i.e. N-salicylidene-beta-alanine(2-), (L)}, having the composition [Cu(2)(L)(2)(H(2)O)].H(2)O (1), [Cu(L)(H(2)O)](n) (2), and [Zn(L)(H(2)O)](n) (3), have been prepared and characterized by elemental analyses, UV-visible (UV-VIS), FT-IR and ESI-MS spectra, and thermal analyses. Complexes 1 and 2 have been investigated by single crystal X-ray analysis and also by temperature dependent magnetic susceptibility measurements (294-80K). All prepared complexes have been evaluated by the antiperoxynitrite activity assay and alloxan-induced diabetes model. The significant antioxidant and antidiabetic activities have been found in the case of both copper(II) complexes 1 and 2. In spite of first two complexes, the zinc(II) complex 3, as well as the potassium salt of the ligand (KHL) showed only insignificant protective effect against the tyrosine nitration in vitro.
- MeSH
- aldehydy chemie MeSH
- beta-alanin chemie MeSH
- experimentální diabetes mellitus prevence a kontrola MeSH
- financování organizované MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
- hypoglykemika farmakologie chemická syntéza chemie MeSH
- krystalografie rentgenová MeSH
- měď chemie MeSH
- molekulární modely MeSH
- molekulární struktura MeSH
- myši MeSH
- scavengery volných radikálů farmakologie chemická syntéza chemie MeSH
- spektrofotometrie ultrafialová MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- zinek chemie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
Curcumin is the most active component of turmeric. It is believed that curcumin is a potent antioxidant and anti-inflammatory agent. Tetrahydrocurcumin (THC), one of the major metabolites of curcumin, exhibits many of the same physiological and pharmacological activities as curcumin and in some systems may exert greater antioxidant activity than curcumin. The aim of this study was to evaluate the effect of THC on the blood glucose, plasma insulin and fatty acid composition of the total lipids in the liver, kidney and brain of control and streptozotocin (STZ)-nicotinamide diabetic rats. The analysis of fatty acids showed that there was a significant increase in the concentrations of palmitic acid (16:1), stearic acid (18:0) and oleic acid (18:1) in the liver, kidney and brain, whereas the concentrations of linolenic acid (18:3) and arachidonic acid (20:4) were significantly decreased. Oral administration of the THC (80 mg/kg body weight) for 45 days to diabetic rats decreased the concentrations of fatty acids, viz., palmitic, stearic, and oleic acid, whereas linolenic and arachidonic acid were elevated. These results suggest that THC exhibits antidiabetic and antihyperlipidemic effects in STZ-nicotinamide induced diabetic rats. It also prevents the fatty acid changes produced during diabetes. The antidiabetic and antihyperlipidaemic effects of THC are more potent than those of curcumin at the same dose. The results of the present study indicate that THC showed an antihyperlipidemic effect in addition to its antidiabetic effect in type 2 diabetic rats.
- MeSH
- diabetes mellitus 2. typu prevence a kontrola MeSH
- experimentální diabetes mellitus prevence a kontrola MeSH
- interpretace statistických dat MeSH
- inzulin metabolismus terapeutické užití MeSH
- krevní glukóza metabolismus účinky léků MeSH
- kurkumin analogy a deriváty chemie metabolismus MeSH
- mastné kyseliny chemie metabolismus MeSH
- potkani Wistar metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- antioxidancia terapeutické užití MeSH
- experimentální diabetes mellitus prevence a kontrola MeSH
- finanční podpora výzkumu jako téma MeSH
- měď terapeutické užití MeSH
- myši MeSH
- Schiffovy báze terapeutické užití MeSH
- superoxiddismutasa MeSH
- volné radikály MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- alloxan MeSH
- antioxidancia farmakologie MeSH
- bronchospasmus prevence a kontrola MeSH
- experimentální diabetes mellitus prevence a kontrola MeSH
- indomethacin toxicita MeSH
- krysa rodu rattus MeSH
- modely nemocí na zvířatech MeSH
- morčata MeSH
- myši MeSH
- žaludeční sliznice účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- morčata MeSH
- myši MeSH
- zvířata MeSH
