Large screening programs of nasopharyngeal carcinoma (NPC) are still challenging due to the scarcity of the resources in higher prevalence parts of the world. In the present study, the development of a new low cost immunochromatography (ICG) strip test for screening of nasopharyngeal carcinoma is reported. A conjugate of gold nanoparticle-anti viral capsid antigen (VCA) immunoglobulin A (IgA) antibody was used as the detection probe. The test is based on a non-competitive immunoassay for rapid and reliable detection the serum specific IgA of Epstein–Barr virus (EBV) VCA. Patient serum samples were analyzed with the strip and compared with enzyme-linked immunosorbent assay (ELISA) and pathological evaluation. The obtained results from the ICG strips were well correlated with the two reference methods. An evaluation of stability, sensitivity and specificity suggested the strip suitable for NPC screening programs in high-risk peoples and also for the recurrence monitoring.
- MeSH
- časná detekce nádoru * metody trendy využití MeSH
- chromatografie afinitní metody využití MeSH
- ELISA metody využití MeSH
- imunoglobulin A izolace a purifikace krev MeSH
- kovové nanočástice diagnostické užití využití MeSH
- lidé MeSH
- nádorové biomarkery * imunologie izolace a purifikace krev MeSH
- nádory nosohltanu * diagnóza imunologie krev MeSH
- statistika jako téma MeSH
- virus Epsteinův-Barrové enzymologie imunologie izolace a purifikace MeSH
- zlato diagnostické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets in vitro and following transplantation. As T(1) contrast agents (CAs), gadolinium(III) complexes linked to β-cyclodextrin (Gd-F-βCD) or bound to titanium dioxide (TiO2 @RhdGd) were tested. As a T(2) CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with gadolinium(III) CAs were visible as hyperintense spots on MR in vitro, but detection in vivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans in vitro as well as in vivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provides better contrast enhancement within a shorter scan time.
- MeSH
- beta-cyklodextriny chemie MeSH
- fluorescenční barviva chemie MeSH
- fluorescenční mikroskopie MeSH
- gadolinium chemie MeSH
- kontrastní látky chemie diagnostické užití MeSH
- kovové nanočástice chemie diagnostické užití MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- Langerhansovy ostrůvky cytologie metabolismus radiografie MeSH
- magnetická rezonanční tomografie MeSH
- potkani inbrední LEW MeSH
- sloučeniny manganu chemie MeSH
- titan chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- financování organizované MeSH
- kovové nanočástice diagnostické užití MeSH
- krysa rodu rattus MeSH
- magnetismus přístrojové vybavení MeSH
- potkani Wistar MeSH
- testování materiálů metody přístrojové vybavení MeSH
- tkáňová distribuce MeSH
- železité sloučeniny aplikace a dávkování farmakokinetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
