- MeSH
- buněčná diferenciace MeSH
- buněčná sebeobnova MeSH
- buněčné dělení MeSH
- cytofotometrie MeSH
- hematopoetický systém * cytologie embryologie fyziologie MeSH
- kmenové buňky fyziologie MeSH
- krevní buňky cytologie fyziologie klasifikace MeSH
- lidé MeSH
- modely u zvířat MeSH
- myši MeSH
- statistika jako téma MeSH
- transplantace kmenových buněk MeSH
- výzkumné techniky MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Detonation nanodiamonds (DNDs) are usually small particles of 4–5 nm, but in aqueous suspension, DNDs form agglomerates in sizes larger than 1 μm. We propose the use of Bead Assisted Sonic Disintegration and a carboxylation procedure, to reduce DNDs aggregates sizes to approximately 100 nm. High cost zirconium beads have been substituted by silica beads synthetized in our laboratory and less-time consuming conditions were standardized. Techniques as Dynamic Light Scattering (DLS), Fourier Transform InfraRed Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and X-ray Photoelectron Spectroscopy (XPS), have been used to characterize the resulting diamond nanoparticles. While the incubation of Red Blood Cells with partially disaggregated DNDs was used to study whether these nanodiamonds impact in a living system. Our results show the absence of a negative effect in cell viability as well as no differences between Raman spectra of hemoglobin (Hb), from control and cell + DNDs conditions.
- MeSH
- biokompatibilní materiály MeSH
- krevní buňky cytologie fyziologie MeSH
- nanodiamanty * normy MeSH
- Ramanova spektroskopie metody MeSH
- spektroskopie infračervená s Fourierovou transformací metody MeSH
- testování materiálů MeSH
- ultrazvuk metody přístrojové vybavení MeSH
- velikost částic MeSH
- Publikační typ
- práce podpořená grantem MeSH
Fifth edition v, 491 stran : ilustrace ; 25 cm
- MeSH
- hematologické testy metody MeSH
- krevní buňky * fyziologie MeSH
- krevní nemoci MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- hematologie a transfuzní lékařství
230 s. : il., tab. ; 21 cm
- MeSH
- biochemická analýza krve MeSH
- diferenciální diagnóza MeSH
- histocytochemie metody využití MeSH
- imunohistochemie metody využití MeSH
- klinické laboratorní techniky metody využití MeSH
- krevní a lymfatické nemoci diagnóza genetika klasifikace terapie MeSH
- krevní buňky cytologie fyziologie klasifikace patologie MeSH
- Publikační typ
- atlasy MeSH
- příručky MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- hematologie a transfuzní lékařství
Krevní buňky podléhají během odběru, zpracování a skladování významným morfologickým a biochemickým změnám. Struktura a funkce krevních buněk jsou ovlivněny řadou faktorů, počínaje technikou odběru, složením antikoagulačního roztoku, kontaktem s povrchem odběrového vaku či setu, metodou zpracování, obsahem leukocytů v produktu či způsobem deleukotizace. Metabolické a morfologické alterace erytrocytů i trombocytů v koncentrátech limitují jejich skladovatelnost a pravděpodobně mohou ovlivnit terapeutický efekt transfuze. Sdělení předkládá stručný přehled změn, které v krevních buňkách v průběhu skladování probíhají, možnosti monitorování buněčného poškození a shrnuje význam stanovení markerů buněčného poškození pro výzkum, validaci nových postupů a praxi.
Blood cells are subjected to important morphologic and biochemical changes during collection, processing and storage. Their structure and function are influenced by a number of factors, such as collection technique, composition of coagulation solution, contact with the surface of the collection bag or set, method of processing and content of leukocytes in the product or leukodepletion method. Metabolic and morphologic alterations in red blood cells and platelets concentrates limit their storage and may be associated with delayed posttransfusion recovery in vivo. The communication provides a brief summary of changes in blood cells during storage, the possibitity to monitor cell injury and summarizes the significance of markers of cell injury research, validation of new procedures and practice.
Blood cell morphology and count are not uniform across species. Recently, between-species comparisons revealed that the size of red blood cells is associated with body size in some lizard taxa, and this finding was interpreted in the context of the metabolic theory. In the present study, we examined the numbers and the size of blood cells in 2 species of monitor lizards, the mangrove-dwelling monitor (Varanus indicus) and the savannah monitor (V. exanthematicus), and we compared these traits in individuals of different body size. The results revealed that during the course of ontogeny, the size of red blood cells increases with body mass. Because the mass-specific metabolic rate decreases with body size and the cell volume-to-surface ratio decreases with the cell size, changes in the erythrocyte size might be the result of oxygen transport adjustment.
- MeSH
- bazální metabolismus fyziologie MeSH
- druhová specificita MeSH
- erytrocyty cytologie fyziologie MeSH
- ještěři anatomie a histologie klasifikace fyziologie MeSH
- krevní buňky cytologie fyziologie MeSH
- leukocyty cytologie fyziologie MeSH
- spotřeba kyslíku fyziologie MeSH
- velikost buňky * MeSH
- velikost těla * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Phagocytosis is an important function of both insect haemocytes and mammalian blood cells. Linden bugs and cotton leaf worms have been suggested as new alternative models for ecological and drug toxicology but no data on their haemocyte physiology have been published. Our assays with particle ingestion of the NBT test were carried out on prohaemocytes, granulocytes, plasmatocytes and spherulocytes of adult linden bug and cotton leaf worm larvae. We found that phagocytic activity is on average 10% in the linden bug, and 50% in cotton leaf worm haemocytes: the phagocytic index is 3.5 in both species and nitroblue tetrazolium reduction is 0.5 in the linden bug and 3.2 in the cotton leaf worm. Phagocytic charactersitics of the prohaemocytes and granulocytes in the cotton leaf worm are closed to mammalian neutrophil physiology. Our data suggest that cotton leaf worm haemocytes may be a new potential alternative model for screening of phagocytosis.
- Klíčová slova
- Spodoptera littoralis, Pyrrhocoris apterus, NBT test,
- MeSH
- fagocytóza fyziologie MeSH
- financování organizované MeSH
- hemocyty fyziologie imunologie MeSH
- Heteroptera fyziologie imunologie MeSH
- hmyz fyziologie imunologie MeSH
- krevní buňky fyziologie imunologie MeSH
- modely u zvířat MeSH
- Spodoptera fyziologie imunologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
CD34 is the most frequently used marker for the selection of cells for bone marrow (BM) transplantation. The use of CD133 as an alternative marker is an open research topic. The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of CD133+ and CD34+ populations from bone marrow and mobilized peripheral blood. Eight cell populations were compared: CD34+ and CD133+ cells from both the BM (CML Ph-, CML Ph+, and healthy volunteers) and mobilized peripheral blood cells. Multicolor flow cytometry and cultivation experiments were used to measure expression and differentiation of the individual populations. It was observed that the CD133+ BM population showed higher cell expansion. Another finding is that during a 6-day cultivation with 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), more cells remained in division D0 (non-dividing cells). There was a higher percentage of CD38- cells observed on the CD133+ BM population. It was also observed that the studied populations contained very similar but not the same pools of progenitors: erythroid, lymphoid, and myeloid. This was confirmed by CFU-GM and CFU-E experiments. The VEGFR antigen was used to monitor subpopulations of endothelial sinusoidal progenitors. The CD133+ BM population contained significantly more VEGFR+ cells. Our findings suggest that the CD133+ population from the BM shows better proliferation activity and a higher distribution of primitive progenitors than any other studied population.
- MeSH
- antigeny CD34 krev imunologie MeSH
- biologické markery metabolismus MeSH
- buněčná diferenciace imunologie MeSH
- buněčný rodokmen MeSH
- buňky kostní dřeně cytologie fyziologie MeSH
- CD antigeny krev imunologie MeSH
- glykoproteiny krev imunologie MeSH
- hematopoetické kmenové buňky cytologie fyziologie MeSH
- krevní buňky cytologie fyziologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- peptidy krev imunologie MeSH
- proliferace buněk MeSH
- separace buněk MeSH
- vaskulární endoteliální růstový faktor A metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
MicroRNAs (miRNAs), important regulators of cellular processes, show specific expression signatures in different blood cell lineages and stages of hematopoietic stem cell (HSC) differentiation, indicating their role in the control of hematopoiesis. Because neonatal blood displays various features of immaturity, we might expect differential miRNA regulation. Herein, we determined miRNA expression profiles of umbilical cord blood (UCB) cell lineages and compared them to those of bone marrow (BM) and peripheral blood (PB) cell counterparts. Further, we determined mRNA expression profiles using whole-genome microarrays. An approach combining bioinformatic prediction of miRNA targets with mRNA expression profiling was used to search for putative targets of miRNAs with potential functions in UCB. We pointed out several differentially expressed miRNAs and associated their expression with the target transcript levels. miR-148a expression was suppressed in HSCs and its level inversely correlated with the previously verified target, DNA methyltransferase 3B, suggesting dependence of de novo DNA methylation in HSCs on miR-148a. Prolonged cell survival of UCB HSCs may be associated with low expression of miR-143 and miR-145 and up-regulation of their downstream targets (high expression of c-MYC and miR-17-92 and following repression of TGFBR2). In HSCs, we monitored significant up-regulation of eight miRNAs, which were previously verified as regulators of HOX genes. Further, miR-146b may be associated with immaturity of neonatal immune system because it is strongly up-regulated in UCB granulocytes and T lymphocytes compared to PB cell counterparts. Comparative analysis revealed 13 miRNAs significantly altered between UCB and BM CD34(+) cells. In UCB CD34(+) cells, we monitored up-regulation of miR-520h, promoting differentiation of HSCs into progenitor cells, and reduction of miR-214, whose expression might support HSC survival. In conclusion, UCB cells show specific miRNA expression patterns, indicating different regulation in these cells.
- MeSH
- antigeny CD34 metabolismus MeSH
- buněčný rodokmen genetika MeSH
- dospělí MeSH
- fetální krev cytologie metabolismus MeSH
- hematopoetické kmenové buňky metabolismus MeSH
- krevní buňky metabolismus fyziologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA genetika metabolismus MeSH
- novorozenec MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- senioři MeSH
- shluková analýza MeSH
- stanovení celkové genové exprese MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- validační studie MeSH
5th ed. xxvii, 2523 s. : il., tab. ; 29 cm
- MeSH
- fyziologie MeSH
- krevní buňky fyziologie MeSH
- krevní nemoci MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- hematologie a transfuzní lékařství
- fyziologie