Liver cirrhosis is associated with impairment of cardiovascular function including alterations of the heart innervation, humoral and nervous dysregulation, changes in systemic circulation and electrophysiological abnormalities. Choline acetyltransferase (ChAT), enzyme forming acetylcholine, tyrosine hydroxylase (TH), and dopamine-β-hydroxylase (DBH), enzymes participating in noradrenaline synthesis, are responsible for the production of classical neurotransmitters, and atrial natriuretic peptide (ANP) is produced by cardiomyocytes. The aim of this study was to evaluate the influence of experimentally induced hepatic dysfunction on the expression of proANP, ChAT, TH, and DBH in the heart. Hepatic dysfunction was induced by application of thioacetamide (TAA) or by ligation of bile duct. Biochemical parameters of hepatic injury and levels of peroxidation in the liver and heart were measured. Liver enzymes measured in the plasma were significantly elevated. Cardiac level of peroxidation was increased in operated but not TAA group animals. In the left atrium of operated rats, the expression of TH and DBH was lower, while expression of ChAT remained unchanged. In TAA group, no significant differences in the expression of the genes compared to controls were observed. Liver injury induced by ligation leads to an imbalance in the intracardiac innervation, which might impair nervous control of the heart.
- MeSH
- akční potenciály MeSH
- atriální natriuretický faktor krev metabolismus MeSH
- dopamin-beta-hydroxylasa krev metabolismus MeSH
- jaterní cirhóza enzymologie metabolismus patofyziologie MeSH
- játra enzymologie patofyziologie MeSH
- krysa rodu rattus MeSH
- myokard enzymologie metabolismus MeSH
- oxidační stres MeSH
- peroxidace lipidů MeSH
- regulace genové exprese * MeSH
- srdce fyziologie MeSH
- svalová kontrakce MeSH
- tyrosin-3-monooxygenasa krev metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
2 sv. : il., tab. ; 31 cm
These researches will implement in 3 departments: Psychiatric department I. LF UK VFN, Genetic department prof. Macek, Motol, II. LF UK and Physiological department Academy of sciences. This proposal of project is original in several points. Applicationbiorhythms theory to ADHD research is one of first project in this field. Study of 130 families in genetically oriented study of ADHD is representative sample. We can replicate last results (review: Faraone 2005) in sample of 130 families of ADHD children. Correlation these results to study biorhythms of 40 ADHD children and correlation to biochemistry markers represent other interesting points of our study. All centres presented relevant papers in Czech and impact journals.
Tyto výzkumy budou probíhat na 3 pracovištích: Psychiatrická klinika, I.LF UK VFN, Genetické pracoviště, Prof. Macka, Motol, 2.LF UK a Fyziologický ústav Akademie věd. Tento projekt je původní z několika hledisek. Aplikace biorytmů v problematice ADHD byla zkoumána jen zcela ojediněle. Vyšetřujeme reprezentativní soubor 130 rodin s tím, že se snažíme replikovat studii Faraoneho 2005. Korelace biochemických a genetických parametrů nebyla dosud vztažena k reprezentativnímu souboru genetických polymorfismů. Dále projekt obsahuje podrobnější studie DBH jako biochemického markeru noradrenegní transmise. Všechna uvedená centra již dříve publikovala příslušné výsledky v impaktovaných časopisech.
- MeSH
- biologické markery analýza MeSH
- dítě MeSH
- dopamin-beta-hydroxylasa krev MeSH
- hyperkinetická porucha diagnóza terapie MeSH
- magnetická rezonanční tomografie MeSH
- methoxyhydroxyfenylglykol krev MeSH
- polymorfismus genetický MeSH
- psychiatrické posuzovací škály MeSH
- serotonin krev MeSH
- studie případů a kontrol MeSH
- Check Tag
- dítě MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- psychiatrie
- biochemie
- biologie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
BACKGROUND: Developmental study of dopaminergic and noradrenergic systems in child psychiatric disorders are rare. DBH activity is one of noradrenergic biochemical marker that is correlate in psychiatry to clinical and genetic data. OBJECTIVES: The main aim of the present study was to measure DBH activity at the onset of acute schizophrenia and depressive disorder in children and adolescents without pharmacological treatment and to compare these values with DBH activity in healthy controls. The authors also investigated untreated ADHD children. METHODS: We examined 42 control healthy children, 15 children non-treated with acute schizophrenia, 15 non-treated children with acute depressive disorders and 30 non-treated ADHD children, all in age 7-14. Plasma DBH level was provided by Nagatsu (1972; 1974). Depressed children were reexamined after clinical remission. RESULTS: DBH activity is statistically significantly decreased in non-treated depressive disorder and ADHD in children and adolescents. DBH activity is normalised during antidepressant therapy in child depression. Child schizophrenia patients present with normal DBH activity. CONCLUSION: These results are similar to the results that have been observed in adult patients with schizophrenia and depression and in previous studies of DBH activity in children with ADHD. These results also indicate hypoactivity of the noradrenergic system in children with ADHD and depression.
- MeSH
- biologické markery metabolismus MeSH
- dítě MeSH
- dopamin-beta-hydroxylasa krev MeSH
- duševní poruchy krev enzymologie MeSH
- hyperkinetická porucha krev enzymologie MeSH
- lidé MeSH
- mladiství MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- dítě MeSH
- dopamin-beta-hydroxylasa fyziologie krev MeSH
- finanční podpora výzkumu jako téma MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- mladiství MeSH
- modely u zvířat MeSH
- potkani Wistar růst a vývoj MeSH
- vývoj mladistvých fyziologie MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
