In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complement the deficiencies of Saccharomyces cerevisiae stb5Δ mutant. The results of phenotypic analysis suggested that KlSTB5 gene deletion did not sensitize K. lactis cells to oxidative stress inducing compounds but led to Klstb5Δ resistance to 4-nitroquinoline-N-oxide and hygromycin B. Expression analysis indicated that the loss of KlSTB5 gene function induced the transcription of drug efflux pump encoding genes that might contribute to increased 4-nitroquinoline-N-oxide and hygromycin B tolerance. Our results show that KlStb5p functions as negative regulator of some ABC transporter genes in K. lactis.
- MeSH
- 4-nitrochinolin-1-oxid farmakologie MeSH
- delece genu MeSH
- fungální proteiny genetika metabolismus MeSH
- Kluyveromyces účinky léků genetika metabolismus MeSH
- oxidační stres účinky léků MeSH
- regulace genové exprese u hub účinky léků MeSH
- Saccharomyces cerevisiae - proteiny genetika metabolismus MeSH
- Saccharomyces cerevisiae účinky léků genetika metabolismus MeSH
- transkripční faktory genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Plant-derived smoke and certain smoke compounds improve seed germination and enhance seedling growth of many species. Thus, smoke-infused water and the active smoke-derived compounds have the potential to be used in different agricultural and horticultural applications. However, despite these interesting and potentially practical properties, it should also be ascertained whether such compounds may pose a health risk, particularly if they are to be used in the production of food or fodder crops. Amongst some of the aspects that would be important to understand are any possible genotoxic properties that the compounds may possess due to potential carry-over effects. Here, we report on a genotoxicity study of 3,4,5-trimethylfuran-2(5H)-one, a compound from plant-derived smoke previously shown to have germination inhibitory activity. Using two in vitro tests, namely the bacterial VITOTOX® test (with/without S9 metabolic activation) and the cytome assay on human C3A cells, no genotoxicity or toxicity was found. Furthermore, these results support a previous study where a related smoke-derived compound with germination promoting properties was investigated.
- MeSH
- 4-nitrochinolin-1-oxid toxicita MeSH
- benzopyreny toxicita MeSH
- chinolony toxicita MeSH
- furany farmakologie MeSH
- hepatocyty cytologie účinky léků metabolismus MeSH
- klíčení účinky léků MeSH
- lidé MeSH
- luciferasy genetika metabolismus MeSH
- mutageny farmakologie MeSH
- nádorové buněčné linie MeSH
- reportérové geny MeSH
- rostliny účinky léků MeSH
- Salmonella typhimurium účinky léků genetika růst a vývoj MeSH
- semena rostlinná účinky léků růst a vývoj MeSH
- SOS odpověď (genetika) účinky léků MeSH
- testy genotoxicity MeSH
- transkripční faktory genetika metabolismus MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Genotoxická aktivita dvojjadrových [Cu2(RCOO)4(N-oxide)2] komplexov (R = 2-chlórfenoxymetyl) bola testovaná voči kmeňom Salmonella typhimurium (TA97, TA100 a TA102) Amesovou metódou in vitro a porovnávaná s aktivitou volných N-oxidov - 4-nitrochinolín-N-oxidom, 4-nitropyridín-N-oxidom a 2-metyl-4-nitropyridín-N-oxidom. Komplexáciou 4-nitrochinolín-N-oxidu s bis(2-chlórfenoxyacetáto)meďnatým fragmentom bol redukovaný jeho mutagénny účinok (9-krát, vzťahované na obsah N-oxidu). V prípade 4-nitropyridín-N-oxidu, 2-metyl-4-mtropyridín-N-oxidu a ich bis(N-oxid)-tetrakis (2-chlórfenoxyacetáto)mednatých komplexov nebol tento fenomén pozorovaný.
Using Ames method in vitro, the genotoxic activity of binuclear [Cu2(RCOO)4(N-oxide)2] complexes (R = 2-chlorophenoxymethyl) was tested against the strains of Salmonella typhimurium (TA97, TA100 and TA102) and compared to that of the free N-oxides - 4-nitroquinoline N-oxide, 4-nitropyridine N-oxide and 2-methyl-4-mtiopyridine N-oxide. Complexation of 4-nitroquinohne N-oxide with bisC2-chlorophenoxyacetato)copper(II) fragment reduced its mutagenic effect (9-times, correlated with N-oxide content). In the case of 4-nitropyridine N-oxide, 2-methyl-4-nitropyridine N-oxide and their bis(N-oxide)tetrakis(2-chlorophenoxyacetato)copper(II) complexes this phenomenon was not observed.
- MeSH
- 4-nitrochinolin-1-oxid aplikace a dávkování MeSH
- chlorfenoly analogy a deriváty aplikace a dávkování MeSH
- měď analogy a deriváty aplikace a dávkování MeSH
- organokovové sloučeniny aplikace a dávkování MeSH
- Salmonella typhimurium MeSH
- techniky in vitro MeSH
- testy genotoxicity MeSH
- testy toxicity MeSH
