OBJECTIVE: A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS: The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus. CONCLUSION: The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.
- MeSH
- biologické markery MeSH
- cytogenetické vyšetření MeSH
- imunofenotypizace MeSH
- imunoglobuliny genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- plazmocelulární leukemie diagnóza metabolismus patologie MeSH
- proliferace buněk MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman. RESULTS: Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines - t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency. CONCLUSION: The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy.
- MeSH
- buňky kostní dřeně účinky léků patologie fyziologie MeSH
- cytogenetické vyšetření MeSH
- interleukin-6 farmakologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mnohočetný myelom patologie MeSH
- nádorové buněčné linie MeSH
- primární buněčná kultura * metody MeSH
- proliferace buněk účinky léků MeSH
- recidiva MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- plazmatická koncentrace léčiva,
- MeSH
- analgetika MeSH
- antiastmatika farmakokinetika farmakologie krev škodlivé účinky MeSH
- antibakteriální látky farmakokinetika farmakologie krev MeSH
- antidepresiva farmakokinetika farmakologie krev MeSH
- antifungální látky farmakologie krev MeSH
- antikonvulziva farmakologie krev MeSH
- antipyretika MeSH
- cytostatické látky farmakologie krev MeSH
- farmakoterapie metody trendy MeSH
- hodnocení rizik MeSH
- imunosupresiva farmakologie krev škodlivé účinky MeSH
- individualizovaná medicína trendy MeSH
- kardiovaskulární látky farmakologie krev MeSH
- lidé MeSH
- monitorování léčiv * klasifikace metody statistika a číselné údaje trendy MeSH
- trankvilizéry farmakokinetika farmakologie krev MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- abstrakt z konference MeSH
