"NR8991" Dotaz Zobrazit nápovědu
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il. ; 30 cm
The aim of the study is to evaluate the effect of acute hyperinsulinemia on MR Spectroscopy of the brain tissue, neurosteriod and adipokine metabolism in diabetic, insulin-resistant and healthy subjects. The aim of the study is to evaluate the effect ofacute hyperglycemia on MR Spectroscopy of the brain tissue, neurosteriod and adipokine metabolism in diabetic, insulin-resistant and healthy subjects. The aim of the study is to evaluate the effect of acute hypertriglyceridemia on MR Spectroscopy of thebrain tissue, neurosteriod and adipokine metabolism in diabetic, insulin-resistant and healthy subjects
Náplní studie je sledování vlivu akutní hyperinzulinémie na vybrané aspekty mozkového metabolismu u osob s diabetem 1. typu, inzulinovou rezistencí a u zdravých kontrol. osob. Bude hodnocen MR spektroskopický obraz mozku, metabolismus neurosteriodů a vybSledování vlivu akutní hyperglykémie na vybrané aspekty mozkového metabolismu u osob s diabetem 1. typu, inzulinovou rezistencí a u zdravých osob. Bude hodnocen MR spektroskopický obraz mozku, metabolismus neurosteriodů a vybraných adipokinů. Sledovánívlivu akutní hypertrigylceridémie na vybrané aspekty mozkového metabolismu u osob s diabetem 1. typu, inzulinovou rezistencí a u zdravých osob. Bude hodnocen MR spektroskopický obraz mozku, metabolismus neurosteriodů a vybraných adipokinů.
- MeSH
- adipokiny analýza MeSH
- diabetes mellitus 1. typu patofyziologie MeSH
- hematoencefalická bariéra MeSH
- hyperinzulinismus patofyziologie MeSH
- inzulinová rezistence MeSH
- magnetická rezonanční spektroskopie MeSH
- metabolismus účinky léků MeSH
- mozek - chemie MeSH
- nemoci centrálního nervového systému metabolismus MeSH
- neurotransmiterové látky analýza MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- diabetologie
- radiologie, nukleární medicína a zobrazovací metody
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Our aim was to assess the reaction of TNFalpha, resistin, leptin and adiponectin to lipid infusion. Eight healthy subjects underwent a 24-hour lasting infusion of lipid emulsion. Plasma concentrations and expressions of selected cytokines in subcutaneous fat were measured. TNFalpha plasma concentration did not change during the first 4 hours of hypertriglyceridemia, but a significant increase after 24 hours was detected (p<0.001 for 0; 30; 240 min vs. 24 h). Plasma concentration of resistin significantly increased at 30 min of infusion and remained elevated (p<0.01 for 0 min vs. 30; 240 min; p<0.001 for 0 min vs. 24 h). Plasma concentrations of leptin and adiponectin did not show any significant changes. Although the expression of resistin in the subcutaneous adipose tissue tended to increase, the change was not significant. Expressions of TNFalpha, leptin and adiponectin were unaffected. In conclusions, our results indicate that acutely induced hyperlipidemia could influence the secretion of TNFalpha and resistin.
- MeSH
- adipokiny genetika krev MeSH
- adiponektin krev MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- dospělí MeSH
- financování organizované MeSH
- hyperlipidemie genetika chemicky indukované krev MeSH
- intravenózní infuze MeSH
- kyseliny mastné neesterifikované krev MeSH
- leptin krev MeSH
- lidé MeSH
- mladý dospělý MeSH
- podkožní tuk metabolismus MeSH
- regulace genové exprese MeSH
- resistin krev MeSH
- TNF-alfa krev MeSH
- triglyceridy krev MeSH
- tukové emulze intravenózní aplikace a dávkování MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
Adipocyte/macrophage fatty acid binding protein (A-FABP) has been shown to be closely associated with metabolic syndrome, obesity and development of atherosclerosis. Moreover, A-FABP has been recently suggested as a potential therapeutic target of these abnormalities in animal models. The present review aims to summarize current knowledge on A-FABP functions and regulations both in animal models and humans, since the role of A-FABP in human physiology and disease has not been presently clarified.
The hyperinsulinemic euglycemic clamp (HEC) combined with indirect calorimetry (IC) is used for estimation of insulin-stimulated substrate utilization. Calculations are based on urinary urea nitrogen excretion (UE), which is influenced by correct urine collection. The aims of our study were to improve the timing of urine collection during the clamp and to test the effect of insulin on UE in patients with type 1 diabetes (DM1; n=11) and healthy subjects (C; n=11). Urine samples were collected (a) over 24 h divided into 3-h periods and (b) before and during two-step clamp (1 and 10 mIU.kg(-1).min(-1); period 1 and period 2) combined with IC. The UE during the clamp was corrected for changes in urea pool size (UEc). There were no significant differences in 24-h UE between C and DM1 and no circadian variation in UE in either group. During the clamp, serum urea decreased significantly in both groups (p<0.01). Therefore, UEc was significantly lower as compared to UE not adjusted for changes in urea pool size both in C (p<0.001) and DM1 (p<0.001). While UE did not change during the clamp, UEc decreased significantly in both groups (p<0.01). UEc during the clamp was significantly higher in DM1 compared to C both in period 1 (p<0.05) and period 2 (p<0.01). The UE over 24 h and UEc during the clamp were statistically different in both C and DM1. We conclude that urine collection performed during the clamp with UE adjusted for changes in urea pool size is the most suitable technique for measuring substrate utilization during the clamp both in DM1 and C. Urine collections during the clamp cannot be replaced either by 24-h sampling (periods I-VII) or by a single 24-h urine collection. Attenuated insulin-induced decrease in UEc in DM1 implicates the impaired insulin effect on proteolysis.
- MeSH
- analýza moči metody MeSH
- analýza rozptylu MeSH
- časové faktory MeSH
- chemická stimulace MeSH
- diabetes mellitus 1. typu diagnóza metabolismus moč MeSH
- dospělí MeSH
- dusík močoviny v krvi MeSH
- dusík moč MeSH
- energetický metabolismus fyziologie MeSH
- glykemický clamp metody MeSH
- inzulin aplikace a dávkování MeSH
- krevní glukóza metabolismus MeSH
- lidé MeSH
- močovina moč MeSH
- nepřímá kalorimetrie MeSH
- odběr biologického vzorku metody MeSH
- referenční hodnoty MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
Certain androstane steroids (AS) modulate ionotropic receptors, as do the pregnane steroids. Whereas women produce significant amounts of neuroactive progesterone metabolites, the steroid neuromodulators in men originate mainly from the 3-oxo-4-ene C(19)-steroids, which are converted to their 3alpha- and 3beta-hydroxy-5alpha/5beta-reduced metabolites. The neuromodulating effects of AS prompted us to monitor circulating levels of the steroids to estimate metabolic pathways in the periphery that may influence brain concentrations of AS. Hence, the serum levels of 20 steroids and 16 steroid polar conjugates including 17-oxo- and 17beta-hydroxy-derivatives of 5alpha/beta-androstane-3alpha/beta-hydroxy-androstane steroids were quantified in 15 men (16-62 years of age) using GC-MS. The conjugated AS for the most part reached micromolar concentrations, these being two or three orders of magnitude higher than those of the free steroids. The ratios of conjugates to free steroids were one to two orders of magnitude higher than the values for the corresponding pregnane steroids. This data suggested that conjugation may considerably restrain the transport of free AS from the periphery into the central nervous system.
- MeSH
- androstany chemie krev MeSH
- androstendion chemie MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- pregnany krev MeSH
- teplota MeSH
- testosteron MeSH
- tlak MeSH
- trimethylsilylové sloučeniny analýza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
