Species of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex are important human pathogens which can be recovered from animals and food, potential sources for their dissemination. The aim of the present study was to characterise the Acinetobacter isolates recovered from market meat samples in Peru. From July through August 2012, 138 meat samples from six traditional markets in Lima were cultured in Lysogeny and Selenite broths followed by screening of Gram-negative bacteria in selective media. Bacterial isolates were identified by MALDI-TOF MS and DNA-based methods and assessed for their clonal relatedness and antimicrobial susceptibility. Twelve Acinetobacter isolates were recovered from calf samples. All but one strain were identified as members of the clinically-relevant Acinetobacter calcoaceticus-Acinetobacter baumannii complex: 9 strains as Acinetobacter pittii, 1 strain as A. baumannii, and 1 strain as the recently described novel species A. dijkshoorniae. The remaining strain could not be identified at the species level unambiguously but all studies suggested close relatedness to A. bereziniae. All isolates were well susceptible to antibiotics. Based on macrorestriction analysis, six isolates were further selected and some of them were associated with novel MLST profiles. The presence of pathogenic Acinetobacter species in human consumption meat might pose a risk to public health as potential reservoirs for their further spread into the human population. Nevertheless, the Acinetobacter isolates from meat found in this study were not multidrug resistant and their prevalence was low. To our knowledge, this is also the first time that the A. dijkshoorniae species is reported in Peru.

• MeSH
• Acinetobacter účinky léků   genetika   izolace a purifikace   patogenita   MeSH
• antibakteriální látky farmakologie   MeSH
• infekce bakteriemi rodu Acinetobacter mikrobiologie   veterinární   MeSH
• kontaminace potravin analýza   MeSH
• lidé MeSH
• maso mikrobiologie   MeSH
• mikrobiální testy citlivosti MeSH
• multilokusová sekvenční typizace MeSH
• nemoci skotu mikrobiologie   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Peru MeSH

In recent years, several efforts have been made to develop quick and low cost bacterial identification methods. Genotypic methods, despite their accuracy, are laborious and time consuming, leaving spectroscopic methods as a potential alternative. Mass and infrared spectroscopy are among the most reconnoitered techniques for this purpose, with Raman having been practically unexplored. Some species of the bacterial genus Acinetobacter are recognized as etiological agents of nosocomial infections associated with high rates of mortality and morbidity, which makes their accurate identification important. The goal of this study was to assess the ability of Raman spectroscopy to discriminate between 16 Acinetobacter species belonging to two phylogroups containing taxonomically closely related species, that is, the Acinetobacter baumannii-Acinetobacter calcoaceticus complex (six species) and haemolytic clade (10 species). Bacterial spectra were acquired without the need for any sample pre-treatment and were further analyzed with multivariate data analysis, namely partial least squares discriminant analysis (PLSDA). Species discrimination was achieved through a series of sequential PLSDA models, with the percentage of correct species assignments ranging from 72.1% to 98.7%. The obtained results suggest that Raman spectroscopy is a promising alternative for identification of Acinetobacter species.

• MeSH
• Acinetobacter baumannii chemie   klasifikace   izolace a purifikace   MeSH
• Acinetobacter calcoaceticus chemie   klasifikace   izolace a purifikace   MeSH
• bakteriologické techniky MeSH
• infekce spojené se zdravotní péčí diagnóza   mikrobiologie   MeSH
• klasifikace MeSH
• lidé MeSH
• Ramanova spektroskopie * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Acinetobacter seifertii is a recently described species that belongs to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. It has been recovered from clinical samples and is sometimes associated with antimicrobial resistance determinants. We present here the case of three A. seifertii clinical isolates which were initially identified as Acinetobacter sp. by phenotypic methods but no identification at the species level was achieved using semi-automated identification methods. The isolates were further analysed by whole genome sequencing and identified as A. seifertii. Due to the fact that A. seifertii has been isolated from serious infections such as respiratory tract and bloodstream infections, we emphasize the importance of correctly identifying isolates of the genus Acinetobacter at the species level to gain a deeper knowledge of their prevalence and clinical impact.

• MeSH
• Acinetobacter baumannii genetika   MeSH
• Acinetobacter klasifikace   účinky léků   genetika   izolace a purifikace   MeSH
• antibakteriální látky farmakologie   MeSH
• DNA bakterií genetika   MeSH
• DNA gyráza genetika   MeSH
• genom bakteriální MeSH
• infekce bakteriemi rodu Acinetobacter krev   epidemiologie   mikrobiologie   MeSH
• katétrové infekce mikrobiologie   MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• RNA ribozomální 16S genetika   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Bolívie epidemiologie   MeSH

Bacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm). Notably, the alignment of the newly sequenced phage genomes (40-41 kb of DNA length) and all Acinetobacter podoviruses deposited in Genbank has shown high synteny, regardless of the date and source of isolation that spans from America to Europe and Asia. Interestingly, the C-terminal pectate lyase domain of these phage tail fibres is often the only difference found among these viral genomes, demonstrating a very specific genomic variation during the course of their evolution. We proved that the pectate lyase domain is responsible for phage depolymerase activity and binding to specific Acinetobacter bacterial capsules. We discuss how this mechanism of phage-host co-evolution impacts the tail specificity apparatus of Acinetobacter podoviruses.

• MeSH
• Acinetobacter baumannii virologie   MeSH
• Acinetobacter calcoaceticus virologie   MeSH
• genom virový genetika   MeSH
• hostitelská specificita fyziologie   MeSH
• Podoviridae klasifikace   genetika   metabolismus   MeSH
• polygalakturonasa metabolismus   MeSH
• polysacharid-lyasy metabolismus   MeSH
• proteinové domény fyziologie   MeSH
• sekvence nukleotidů MeSH
• virion genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Asie MeSH
• Evropa MeSH

This study aimed to define the taxonomic status of a phenetically distinct group of 16 strains that corresponds to Acinetobacter genomic species 'close to 13TU', a provisional genomic species of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex recognized by Gerner-Smidt and Tjernberg in 1993. These strains have been isolated in different countries since the early 1990s and were mostly recovered from human clinical specimens. They were compared with 45 reference strains representing the known taxa of the ACB complex using taxonomic methods relevant to the genus Acinetobacter. Based on sequence analysis of the concatenated partial sequences (2976 bp) of seven housekeeping genes, the 16 strains formed a tight and well-supported cluster (intracluster sequence identity of ≥98.4 %) that was clearly separated from the other members of the ACB complex (≤94.7 %). The species status of the group was supported by average nucleotide identity values of ≤91.7 % between the whole genome sequence of representative strain NIPH 973(T) (NCBI accession no. APOO00000000) and those of the other species. In addition, whole-cell matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) MS analyses indicated the distinctness of the group at the protein level. Metabolic and physiological tests revealed several typical features of the group, although they did not allow its reliable differentiation from the other members of the ACB complex. We conclude that the 16 strains represent a distinct novel species, for which we propose the name Acinetobacter seifertii sp. nov. The type strain is NIPH 973(T) ( = CIP 110471(T) = CCUG 34785(T) = CCM 8535(T)).

• MeSH
• Acinetobacter klasifikace   genetika   izolace a purifikace   MeSH
• bakteriální geny MeSH
• DNA bakterií genetika   MeSH
• fylogeneze * MeSH
• genotyp MeSH
• infekce bakteriemi rodu Acinetobacter mikrobiologie   MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• multilokusová sekvenční typizace MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• techniky typizace bakterií MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

MALDI-TOF MS is becoming the technique of choice for rapid bacterial identification at species level in routine diagnostics. However, some drawbacks concerning the identification of closely related species such as those belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) complex lead to high rates of misidentifications. In this work we successfully developed an approach that combines MALDI-TOF MS and chemometric tools to discriminate the six Acb complex species (A. baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, A. calcoaceticus, genomic species "Close to 13TU" and genomic species "Between 1 and 3"). Mass spectra of 83 taxonomically well characterized clinical strains, reflecting the breadth of currently known phenetic diversity within the Acb complex, were achieved from intact cells and cell extracts and analyzed with hierarchical cluster analysis (HCA) and partial least squares discriminant analysis (PLSDA). This combined approach lead to 100% of correct species identification using mass spectra obtained from intact cells. Moreover, it was possible to discriminate two Acb complex species (genomic species "Close to 13TU" and genomic species "Between 1 and 3") not included in the MALDI Biotyper database.

• MeSH
• Acinetobacter baumannii chemie   klasifikace   MeSH
• Acinetobacter calcoaceticus chemie   klasifikace   MeSH
• bakteriologické techniky metody   MeSH
• infekce bakteriemi rodu Acinetobacter diagnóza   mikrobiologie   MeSH
• lidé MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH

MALDI-TOF MS is currently becoming the method of choice for rapid identification of bacterial species in routine diagnostics. Yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, we evaluated a protocol which was different from that used in the Bruker Daltonics identification system (MALDI BioTyper) to improve species identification using a taxonomically precisely defined set of 105 strains representing the four validly named species of the ACB complex. The novel protocol is based on the change in matrix composition from alpha-cyano-4-hydroxycinnamic acid (saturated solution in water:acetonitrile:trifluoroacetic acid, 47.5:50:2.5, v/v) to ferulic acid (12.5mgml(-1) solution in water:acetonitrile:formic acid 50:33:17, v/v), while the other steps of sample processing remain unchanged. Compared to the standard protocol, the novel one extended the range of detected compounds towards higher molecular weight, produced signals with better mass resolution, and allowed the detection of species-specific signals. As a result, differentiation of A. nosocomialis and A. baumannii strains by cluster analysis was improved and 13 A. nosocomialis strains, assigned erroneously or ambiguously by using the standard protocol, were correctly identified.

• MeSH
• Acinetobacter chemie   klasifikace   izolace a purifikace   MeSH
• bakteriologické techniky metody   MeSH
• odběr biologického vzorku metody   MeSH
• shluková analýza MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH

Acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13TU are increasingly recognized as clinically important taxa within the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex. To define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the ACB complex. All strains were characterized by AFLP analysis, amplified rDNA restriction analysis and nutritional or physiological testing, while selected strains were studied by 16S rRNA and rpoB gene sequence analysis, multilocus sequence analysis and whole-genome comparison. Results supported the genomic distinctness and monophyly of the individual species of the ACB complex. Despite the high phenotypic similarity among these species, some degree of differentiation between them could be made on the basis of growth at different temperatures and of assimilation of malonate, l-tartrate levulinate or citraconate. Considering the medical relevance of gen. sp. 3 and gen. sp. 13TU, we propose the formal names Acinetobacter pittii sp. nov. and Acinetobacter nosocomialis sp. nov. for these taxa, respectively. The type strain of A. pittii sp. nov. is LMG 1035(T) (=CIP 70.29(T)) and that of A. nosocomialis sp. nov. is LMG 10619(T) (=CCM 7791(T)).

• MeSH
• Acinetobacter baumannii klasifikace   genetika   izolace a purifikace   MeSH
• Acinetobacter klasifikace   genetika   izolace a purifikace   MeSH
• DNA bakterií genetika   MeSH
• fenotyp MeSH
• fylogeneze MeSH
• genotyp MeSH
• molekulární sekvence - údaje MeSH
• multilokusová sekvenční typizace MeSH
• RNA ribozomální 16S genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Cíl studie: Stanovit časovou osu nárůstu gramnegativních kmenů bakterií na popálených plochách v průběhu hospitalizace. Typ studie: Retrospektivní Materiál a metodika: Do studie bylo zahrnuto 85 pacientů hospitalizovaných na Klinice popálenin a rekonstrukční chirurgie v letech 2006-2008. Základní předpoklady pro zařazení do studie byly: popálení na více než 15 % TBSA [total body surface area], délka hospitalizace minimálně 1 měsíc, věk nad 18 let. U pacientů byl odebírán materiál na mikrobiologické vyšetření 2., 6., 10., 14. a 20. den po přijetí. Výsledky: Celkem bylo u skupiny pacientů izolováno 777 bakteriálních kmenů, z toho 64,6 % spadalo do grampozitivního spektra a 35,4 % do spektra gramnegativních kmenů. Nejčastěji izolovanými grampozitivními patogeny jsou Staphylococcus koagulasa negativní [izolováno 260 kmenů], Bacillus sp. [113 kmenů], v gramnegativním spektru Pseudomonas aeruginosa [81 kmenů], Escherichia coli [63 kmenů] a komplex Acinetobacter calcoaceticus – baumannii [57 kmenů]. Závěr: Ve studii se nám podařilo získat data, která potvrzují teoretický předpoklad o nárůstu počtu gramnegativních kmenů na popálených plochách v průběhu hospitalizace. Ovšem ani 20. den po přijetí k hospitalizaci nejsou tyto kmeny dominantní.

Study objective: To determine the time axis for increase in Gram negative bacterial strains in burn wounds during hospitalization. Study type: Retrospective Material and Methods: Eighty-five patients hospitalized at the Clinic of burns and reconstructive surgery between 2006 and 2008 were enrolled in the study. The major criteria for enrolment were more than 15 % of total body surface area (TBSA) burned, hospital stay of 1 month or more and age over 18 years. Specimens for microbiological examination were collected on days 2, 6, 10, 14 and 20 after admission. Results: A total of 777 bacterial strains were isolated from the study patients, with 64.6 % of these strains being Gram positive and 35.4 % Gram negative. The most frequently isolated Gram positive pathogens were coagulase-negative Staphylococcus (260 strains) and Bacillus sp. (113 strains), while the most common Gram negative pathogens were Pseudomonas aeruginosa (81 strains), Escherichia coli (63 strains) and Acinetobacter calcoaceticus – baumannii complex (57 strains). Conclusion: The study provided data that supports the assumption of increase in Gram negative bacterial strains in burn wounds during hospitalization. Nevertheless, even on day 20 after admission, such strains did not predominate.

• MeSH
• gramnegativní bakterie izolace a purifikace   patogenita   MeSH
• hojení ran MeSH
• infekce v ráně mikrobiologie   MeSH
• lidé MeSH
• popálení komplikace   MeSH
• Check Tag
• lidé MeSH

A total of 85 strains of the genus Acinetobacter were isolated from haemocultures at the Institute of Microbiology of the Teaching Hospital in Olomouc over the period January 1993 to June 1997. Sixty-two (73.0%) strains of the Acinetobacter calcoaceticus-baumannii complex (Acb complex) were the most frequent. In 3 (3.5%) strains it was impossible to decide whether they belonged to the Acb complex. Other acinetobacter species were represented by 20 (23.5%) strains. The greatest amount (28.2%) of these strains was collected from the Clinic of Internal Medicine. Leukemias, lymphomas and myelodysplastic syndromes were the most frequent clinical diagnoses (20.0%) of the patients with a positive haemoculture. The most effective antimicrobial preparations tested were as follows: meropenem (98.8% of susceptible strains), colistin (94.1%), quinolones (90.6-94.1% according to the type of agent) and amikacin (91.8%). The Acb complex strains were less susceptible to antimicrobial agents than other acinetobacters. Production of inductive chromosomal beta-lactamases AmpC was proved in 42 (49.4%) strains whilst no occurrence of extended-spectrum beta-lactamases (ESBL) in the isolated organisms was recorded.

• MeSH
• Acinetobacter klasifikace   účinky léků   izolace a purifikace   MeSH
• antibakteriální látky farmakologie   MeSH
• beta-laktamasy biosyntéza   MeSH
• beta-laktamy MeSH
• fenotyp MeSH
• infekce bakteriemi rodu Acinetobacter krev   mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• nemocnice fakultní MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH