Clear cell renal cell carcinoma (CRCC) is well known for its intratumoral heterogeneity. Paneth-like cells (PLC) have been reported in variable organs (i.e., hepatobiliary, genitourinary, and female genital tract). In genitourinary system, it is possible to find PLCs in epididymis, urinary bladder and prostate. The objective of this study was to assess PLC in CRCCs 13 CRCCs with prominent PLC (CRCCPLC) were selected out of 1378 CRCCs in our registry. The tumors were analyzed using morphologic, immunohistochemical, ultrastructural, and molecular genetic methods. CRCCPLCs were mostly of low histologic grade (12/13). Immunohistochemical profile was compatible with classic CRCC. PLC constituted 10 to-70% of the tumor volume (mean 17.7%, median 10%). PLCs did not express neuroendocrine markers (chromogranin, synaptophysin, CD56, INSM-1). Ultrastructurally, PLCs were filled by membrane bounded vesicles of various sizes and were compatible with secretory type of cells. VHL mutation was found in 9/9 cases, and LOH3p was found in 6/8 analyzable cases. Conclusions: PLC morphology can variably be present in "classic" CRCC, even in a substantial proportion. Ultrastructurally, PLCs have all attributes of secretory cells. Preliminary follow up data showed that these tumors may not be associated with aggressive clinical behavior.

• MeSH
• dospělí MeSH
• imunohistochemie MeSH
• karcinom z renálních buněk patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory ledvin patologie   MeSH
• Panethovy buňky patologie   MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Paneth-like cells (PLCs) are different from Paneth cells (PCs) and contain Paneth-like granules, which have been reported in non-neoplastic conditions and in neoplasms of various organs. PLCs have been reported in clear cell renal cell carcinoma (CCRCC), but not in non-CCRCC, including acquired cystic disease-associated renal cell carcinoma (ACD-RCC). We analyzed clinicopathological features of 24 acquired cystic disease-associated renal cell carcinoma (ACD-RCC) with PLCs (ACD-RCCP+) and compared with those of 23 ACD-RCCs without PLCs (ACD-RCCP-). Approximately half of ACD-RCCs had PLCs and that almost all kidneys harboring ACD-RCC had cysts with PLCs. The fact that many ACD-RCCs and the cysts had PLCs is further evidence that the cyst with vacuoles and complex architecture might be a precursor lesion for ACD-RCC. The presence of PLCs may provide additional morphologic clue for distinguishing ACD-RCC from PRCC in challenging differential diagnostic workup in acquired cystic disease of the kidney setting.

• MeSH
• cystická onemocnění ledvin komplikace   patologie   MeSH
• cysty patologie   MeSH
• diferenciální diagnóza MeSH
• dospělí MeSH
• karcinom z renálních buněk diagnóza   patologie   MeSH
• ledviny patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory ledvin patologie   MeSH
• oxaláty analýza   MeSH
• Panethovy buňky patologie   MeSH
• progrese nemoci MeSH
• senioři MeSH
• staging nádorů metody   MeSH
• TNF-alfa metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Popisovány jsou dvě léze mléčné žlázy s nesmírně vzácnou diferenciací (metaplazií), napodobující acinické buňky serózní slinné žlázy. Jednalo se o ženy ve věku 71 a 40 let; u první jsme rysy podobné acinickému karcinomu nalezli v invazivní komponentě solidního intraduktálního papilokarcinomu, u druhé ženy se jednalo o rozsáhlé změny v rámci běžné intraduktální hyperplazie náhodně zjištěné při exstirpaci fibroadenomu. V obou pozorováních se shodně vyskytovaly hojné epitelie s četnými, v barvení PAS pozitivními, intracytoplazmatickými granuly, která reagovala masivně s protilátkou proti lysozymu a v ultrastruktuře měla jednoznačnou podobu zymogenních granul. Diskutována je diferenciální diagnostika neoplastických proliferací prsu s oxyfilní a granulovanou cytoplazmou a na pozadí literárních faktů a jedinečného nálezu této diferenciace v běžné duktální hyperplazii jsou zvažovány biologické implikace.

Described are two epithelial lesions of the breast displaying extremely rare, widespread acinic cell-like differentiation (metaplasia). Two women, 70 and 40-year-old, one with invasive ductal papillocarcinoma, the other one with conventional intraductal hyperplasia without atypia, both demonstrated massive diffuse, PAS positive, granular eosinophilic transformation of the cell cytoplasm. This unusual cell appearance closely simulated acinar cells in normal serous salivary gland/acinic cell carcinoma or Paneth cells. Both extensive expression of lysozyme and finding of numerous zymogen granules ultrastructurally confirmed the acinic cell-like fenotype. Discussed is differential diagnosis of the breast neoplasm containing overt eosinophilic and granular cytoplasm. Reviewing literature and comparing our unique finding of unusual salivary-type differentiation in conventional ductal hyperplasia of the breast, biologic implications are considered.

• MeSH
• acinární karcinom patologie   MeSH
• duktální karcinom prsu patologie   MeSH
• lidé MeSH
• nádory prsu patologie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• Klíčová slova
• střevní bariéra,
• MeSH
• akutní nemoc MeSH
• dysbióza MeSH
• enterální výživa škodlivé účinky   MeSH
• imunitní systém imunologie   MeSH
• kathelicidiny fyziologie   imunologie   MeSH
• lidé MeSH
• nekrotizující enterokolitida * etiologie   imunologie   patofyziologie   MeSH
• novorozenec nedonošený MeSH
• novorozenec s nízkou porodní hmotností MeSH
• novorozenec MeSH
• Panethovy buňky fyziologie   imunologie   MeSH
• rizikové faktory MeSH
• střevní mikroflóra * imunologie   MeSH
• střevní sliznice * fyziologie   imunologie   růst a vývoj   MeSH
• trávicí systém - fyziologické jevy imunologie   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• práce podpořená grantem MeSH
• přehledy MeSH

The classification of salivary gland tumors is ever-evolving with new variants of tumors being described every year. Next-generation sequencing panels have helped to prove and disprove prior assumptions about tumors' relationships to one another, and have helped refine this classification. Adenoid cystic carcinoma (AdCC) is one of the most common salivary gland malignancies and occurs at all major and minor salivary gland and seromucous gland sites. Most AdCC are predominantly myoepithelial and basaloid with variable cribriform, tubular, and solid growth. The luminal tubular elements are often less conspicuous. AdCC has largely been characterized by canonical MYB fusions, with MYB::NFIB and rarer MYBL1::NFIB. Anecdotal cases of AdCC, mostly in nonmajor salivary gland sites, have been noted to have unusual patterns, including squamous differentiation and macrocystic growth. Recently, this has led to the recognition of a subtype termed "metatypical adenoid cystic carcinoma." Another unusual histology that we have seen with a wide range of architecture, is striking tubular hypereosinophilia. The hypereosinophilia and luminal cell prominence is in stark contrast to the vast majority of AdCC that are basaloid and myoepithelial predominant. A total of 16 cases with tubular hypereosinophilia were collected, forming morular, solid, micropapillary, and glomeruloid growth, and occasionally having rhabdoid or Paneth-like cells. They were subjected to molecular profiling demonstrating canonical MYB::NFIB (5 cases) and MYBL1::NFIB (2 cases), as well as noncanonical EWSR1::MYB (2 cases) and FUS::MYB (1 case). The remaining 6 cases had either no fusion (3 cases) or failed sequencing (3 cases). All cases were present in nonmajor salivary gland sites, with seromucous glands being the most common. These include sinonasal tract (7 cases), laryngotracheal (2 cases), external auditory canal (2 cases), nasopharynx (1 case), base of tongue (2 cases), palate (1 case), and floor of mouth (1 case). A tissue microarray of 102 conventional AdCC, including many in major salivary gland sites was examined for EWSR1 and FUS by fluorescence in situ hybridization and showed that these novel fusions were isolated to this histology and nonmajor salivary gland location. In summary, complex and striking tubular hypereosinophilia and diverse architectures are present within the spectrum of AdCC, particularly in seromucous gland sites, and may show variant EWSR1/FUS::MYB fusions.

• MeSH
• adenoidně cystický karcinom * genetika   patologie   MeSH
• eozinofilie * MeSH
• fúzní onkogenní proteiny genetika   MeSH
• hybridizace in situ fluorescenční MeSH
• lidé MeSH
• nádory slinných žláz * genetika   patologie   MeSH
• protein EWS vázající RNA genetika   MeSH
• protein FUS vázající RNA MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH