Q57330740
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The susceptibility toward antibiotics was determined by disc and MIC methods in Lactobacillus and, for comparison, in Escherichia coli strains isolated from cloacal swabs of broiler chickens derived from various farms in Slovakia. The occurrence of acquired tetracycline resistance in E. coli and lactobacilli isolated from the same sample was similar. The presence of tet(M), tet(S), tet(L) and ermB genes was demonstrated in lactobacilli while the tet(M) gene was not detected in E. coli.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny MeSH
- bakteriální léková rezistence MeSH
- Escherichia coli izolace a purifikace účinky léků MeSH
- financování organizované MeSH
- kloaka mikrobiologie MeSH
- kur domácí MeSH
- Lactobacillus izolace a purifikace účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- polymerázová řetězová reakce MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- bachor cytologie mikrobiologie MeSH
- bakteriální adheze MeSH
- epitel mikrobiologie MeSH
- epitelové buňky MeSH
- koncentrace vodíkových iontů MeSH
- skot MeSH
- Streptococcus bovis klasifikace metabolismus ultrastruktura MeSH
- techniky typizace bakterií MeSH
- uhlovodíky farmakologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
The development of resistance to quinolones (nalidixic acid, ciprofloxacin and enrofloxacin) in 2006-2008 was evaluated in 317 strains of Escherichia coli isolated from healthy chicken broilers from various farms. The isolates (2006/2007/2008) showed a high resistance to nalidixic acid (87/85/67 %), ciprofloxacin (CIP) (49/54/29 %) and enrofloxacin (ENR) (52/42/22 %). Nalidixic acid-resistant isolates with low level of MIC for CIP and ENR represented a single mutation; intermediary MIC for CIP and ENR were related to two mutations and high level resistance MIC for CIP (> or =4 mg/L) and ENR (> or =16 mg/L) represented three mutations (two in gyrA and one in parC). There was a correlation between the phenotype reading of high-level resistance and mutations in gyrA (Ser83Leu, Asp87Tyr or Asp87Asn) and parC (Ser80Ile) gene. Plasmid-mediated quinolone-resistance qnrS gene was detected in one Escherichia coli strain with a high level of ciprofloxacin resistance. Our results demonstrate the increase in occurrence of multiresistant E. coli strains with a high level of chromosomal and plasmid resistance to fluoroquinolones.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální chromozomy MeSH
- bakteriální léková rezistence MeSH
- chinolony farmakologie MeSH
- DNA gyráza genetika MeSH
- DNA-topoisomerasa IV genetika MeSH
- Escherichia coli izolace a purifikace účinky léků MeSH
- financování organizované MeSH
- kur domácí mikrobiologie MeSH
- mikrobiální testy citlivosti MeSH
- missense mutace MeSH
- molekulární sekvence - údaje MeSH
- plazmidy MeSH
- proteiny z Escherichia coli genetika MeSH
- sekvence aminokyselin MeSH
- substituce aminokyselin MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- antibakteriální látky aplikace a dávkování farmakokinetika MeSH
- dobromysl (rod) enzymologie MeSH
- Escherichia coli růst a vývoj MeSH
- financování vládou MeSH
- kur domácí mikrobiologie MeSH
- Lactobacillus růst a vývoj MeSH
- oleje rostlin farmakokinetika MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Vydání první 90 stran : ilustrace, tabulky ; 30 cm
- MeSH
- hygiena MeSH
- kontrola potravin MeSH
- potravinářská technologie MeSH
- potraviny MeSH
- zákonodárství potravinářské MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- hygiena
- zemědělství a potravinářství
- NLK Publikační typ
- učebnice vysokých škol
The influence was evaluated of post-weaning normal nutrition and over-nutrition upon the development of the intestinal microbiota, the alkaline phosphatase activity (AP) and occurrence of obesity in male Sprague-Dawley rats (from days 21 to 40 the control rats were submitted to ad libitum intake of a standard laboratory diet whereas overfed rats received the same diet supplemented with milk-based high fat liquid diet). The jejunal numbers of two dominant divisions of bacteria, i.e. Firmicutes (Lactobacillus/ Enterococcus--LAB) and the Bacteroidetes (Bacteroides/Prevotella--BAC), were determined using the fluorescent in situ hybridization (FISH) method, and the jejunal AP activity was assayed histochemically. On day 40, the overfed rats in comparison with control animals displayed increased adiposity accompanied by enhanced AP activity, abundance of LAB, lower amounts of BAC and, thereafter, higher LAB/BAC ratio (L/B). The numbers of LAB and L/B index positively correlated with body fat, energy intake and AP activity, whereas numbers of BAC showed an opposite tendency. These results revealed the significance of nutritional imprint upon the post-weaning development of intestinal microbial and functional axis and contribute to better understanding of their involvement in energy-balance control and in adverse and/or positive regulation of adiposity.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- Bacteroidetes izolace a purifikace klasifikace MeSH
- biodiverzita MeSH
- dieta MeSH
- financování organizované MeSH
- grampozitivní bakterie izolace a purifikace klasifikace MeSH
- histocytochemie MeSH
- hybridizace in situ fluorescenční MeSH
- jejunum enzymologie mikrobiologie MeSH
- krysa rodu rattus MeSH
- obezita mikrobiologie MeSH
- potkani Sprague-Dawley MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
