Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are widely used for their antiadherence and antioxidative properties. Little is known however about their effects on clinical chemistry markers after long-term consumption. This study was conducted to evaluate the effect of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and PACRAN on the antioxidative status of rodents, divided into three experimental groups. The products were given as dietary admixtures (1500 mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups 2-4) and a control Group 1 which received only stock feed. There were no significant cranberry treatment-related effects on oxidative stress parameters, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase, total antioxidant capacity, thiobarbituric acid reactive substances, advanced oxidation protein products, total SH-groups, or any other measured clinical chemistry markers. Hematological parameters, body weight, and food consumption were also unaffected by intake of cranberries. Only liver glutathione reductase activity and glutathione levels were significantly lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was significantly decreased in Group 2. No gross pathology, effects on organ weights, or histopathology were observed. No genotoxicity was found, and total cytochrome P450 level in liver was unaffected in all groups. The levels of hippuric acid and several phenolic acids were significantly increased in plasma and urine in Groups 2-4. The concentration of anthocyanins was under the detection threshold. The dietary addition of cranberry powders for 14 weeks was well tolerated, but it did not improve the antioxidative status in rats.

• MeSH
• antioxidancia aplikace a dávkování   farmakologie   MeSH
• glutathionperoxidasa metabolismus   MeSH
• katalasa metabolismus   MeSH
• krysa rodu rattus MeSH
• oxidační stres účinky léků   MeSH
• pilotní projekty MeSH
• potkani Wistar MeSH
• rostlinné přípravky aplikace a dávkování   farmakologie   MeSH
• Vaccinium macrocarpon chemie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Research Support, N.I.H., Extramural MeSH

• Publikační typ
• abstrakty MeSH

Anthocyanins from the fruit Lonicera caerulea L. var. kamtschatica (blueberry honeysuckle, Caprifoliaceae) were studied via (semi)preparative chromatographic fractionation followed by MS and μLC/MS analysis. The extraction procedure was optimized with respect to analytical purposes as well as its potential use for the preparation of nutraceuticals. The highest yield of anthocyanins was obtained using acidified methanol as the extraction medium. A comparable total anthocyanin content was obtained using a mixture of methanol and acetone. However, when Lonicera anthocyanins were in contact with acetone, a condensation reaction occurred to a large extent and related 5-methylpyranoanthocyanins were found. The effect of other extraction media, including ethanol as a "green" solvent, is also discussed. The potential of two fractionation procedures for extract purification differing in their chromatographic selectivity and scale was studied (i.e. using a Sephadex LH-20 gel column and a reversed phase). Fractions obtained by both procedures were used for a detailed analysis. MS and μLC/MS(2) methods were used for monitoring anthocyanin and 5-methylpyranoderivatives content as well as identifying less common and more complex dyes (dimer of cyanidin-3-hexoside, cyanidin-ethyl-catechin-hexosides, etc.). These more complex dyes are most likely formed during fruit treatment.

• MeSH
• anthokyaniny analýza   izolace a purifikace   MeSH
• chemická frakcionace přístrojové vybavení   metody   MeSH
• Lonicera chemie   MeSH
• rostlinné extrakty analýza   izolace a purifikace   MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• vysokoúčinná kapalinová chromatografie přístrojové vybavení   metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH

Cílem studie bylo ověřit účinnost kombinace glukosamin sulfátu a chondroitin sulfátu (Proenzi Premium, Walmark) samostatně nebo v kombinaci s rehabilitací. Do studie bylo zařazeno 60 pacientů ve věku 36-70 let s potvrzenou gonartrozou II. az II. rtg stadia. Pacienti byli roztříděni do tří skupin. První skupina byla kontrolní, bez medikace, bez rehabilitace, druhá a třetí skupina užívala glukosamin sulfát 1500 mg a chondrotin sulfát 1200mg denně perorálně po dobu tří měsíců bez rehabilitace, resp. s rehabilitací. Na sledování účinku se použil klinický test WOMAC, visuální analogová škála CVAS) bolesti kolenního kloubu, přítomnost otoku, palpační bolestivost mediálního kondylu femoru a rozsah pohybu - flexe. Hodnocení probíhalo ve třech měřeních (vstupní, po 3 měsících, po 6 měsících). Při užívání kombinace glukosaminu a chondrotiťmu se významně redukovaly některé symptomy osteoartrózy. Došlo k významnému snížení pocitu bolesti (jak dle indexu WOMAC, tak VAS) a indexu pohyblivosti WOMAC. Ve skupině s medikací bez rehabilitace došlo navíc ke snížení indexu ztuhlosti. Současná rehabilitace neměla vliv na ztuhlost, měla ale naopak příznivější vliv na rozsah flexe.

• Klíčová slova
• Proenzi Premium,
• MeSH
• artróza kolenních kloubů farmakoterapie   MeSH
• bolest farmakoterapie   klasifikace   MeSH
• chondroitinsulfáty aplikace a dávkování   terapeutické užití   MeSH
• financování organizované MeSH
• glukosamin aplikace a dávkování   terapeutické užití   MeSH
• kombinovaná farmakoterapie MeSH
• lidé středního věku MeSH
• lidé MeSH
• měření bolesti statistika a číselné údaje   MeSH
• rehabilitace MeSH
• senioři MeSH
• stupeň závažnosti nemoci MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• hodnotící studie MeSH

V práci jsou shrnuty výsledky podávání laktoferrinu obsaženého v přípravku Acneon tablety (firma Walmark, a. s.) u pacientů trpících acne papulopustulosa I.– IV. st. Byl hodnocen účinek jak na nezánětlivé, tak na zánětlivé morfy akné, snášenlivost přípravku a spokojenost pacientů. Výsledkem dvouměsíčního sledování bylo statisticky významné snížení počtu jak komedonů, tak papulopustul.

In this work we summarize the results of lactoferrin (included in Acneon tablets preparate, Walmark) administration in patients suffering from acne papulopustulosa (grade I–IV). We evaluate an effect on both non-inflammatory and inflammatory acne lesions, on tolerance of a preparation and on satisfaction of patients. The outcome of two month surveillance was statistically significant reduction in number of both comedones and papulopustules.

• MeSH
• acne vulgaris farmakoterapie   klasifikace   MeSH
• hodnocení léčiv MeSH
• laktoferrin aplikace a dávkování   MeSH
• mladý dospělý MeSH
• potravní doplňky využití   MeSH
• výsledek terapie MeSH
• Check Tag
• mladý dospělý MeSH
• Publikační typ
• přehledy MeSH

The effect of Lonicera caerulea L. (blue honeysuckle) phenolic fraction (18.5% anthocyanins) on cell viability and against oxidative damage in low density lipoproteins (oxLDL), in rat microsomes and in primary cultures of rat hepatocytes and human umbilical vein endothelial cells (HUVEC), was tested. The phenolic fraction was nontoxic to rat hepatocytes and HUVEC at tested concentrations (1-1000 microg/mL) and time intervals up to 24 h inclusive. Phenolic fraction inhibited rat liver microsome peroxidation, induced by tert-butyl hydroperoxide (tBH), with IC(50) values of 160 +/- 20 microg/mL. The fraction at 0.5, 1.0, and 2.0 microg/mL delayed LDL oxidation, induced by Cu(2+), by 130 +/- 20%, 200 +/- 30%, and 400 +/- 10%, respectively. The treatment of HUVEC with oxidatively modified LDL induced an increase in lactate dehydrogenase (LDH) leakage and thiobarbituric acid reactive substances (TBARS) formation, and resulted in lower formazan formation from 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) uptake, most pronounced for 200 microg/mL (24 h oxidation) after 2 h of incubation. The protective effect of the phenolic fraction against cell damage caused by oxLDL was noted at 0.1 microg/mL for HUVEC and against tBH at 1000 microg/mL for both HUVEC and hepatocytes. The observed protective effects were probably due to the antioxidant properties of L. caerulea constituents, mainly anthocyanins. Microsome peroxidation and LDL oxidation inhibition results provide promising perspectives into the prevention of some oxidative stress-associated diseases. Other data are important in in vitro systems but seem to be accidental in vivo.

• MeSH
• antioxidancia farmakologie   MeSH
• Caprifoliaceae chemie   MeSH
• cévní endotel metabolismus   účinky léků   MeSH
• dospělí MeSH
• hepatocyty metabolismus   účinky léků   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• oxidační stres účinky léků   MeSH
• potkani Wistar MeSH
• rostlinné extrakty farmakologie   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• krysa rodu rattus MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

Purpose of Review This review deals with the botany and chemical composition of Lonicera caerulea L. (honeysuckle) and the biological activity of its main constituents, focusing on potential health benefits of the berries. Findings L. caerulea berries are a rich source of phenolics such as phenolic acids as well as anthocyanins, proanthocyanidins and flavonoids, which show health promoting effects. The consumption of L. caerulea berries may prevent chronic diseases such as diabetes mellitus, cardiovascular diseases and cancer, depending on the content of phenolics in the berries. Conclusions The potential of L. caerulea berries to prevent chronic diseases such as diabetes mellitus, cardiovascular diseases and cancer seems to be related to their phenolics content.

• MeSH
• anthokyaniny farmakologie   MeSH
• fenoly farmakologie   MeSH
• financování organizované MeSH
• flavonoidy farmakologie   MeSH
• fytoterapie MeSH
• Lonicera chemie   MeSH
• rostlinné extrakty MeSH

The fruit of Lonicera caerulea L. (blue honeysuckle; Caprifoliaceae) and its phenolic fraction were analyzed for nutrients and micronutrients. The phenolic fraction was prepared from berries percolated with 0.1% H3PO4 and SPE using Sepabeads SP207. The sugar and lipid content was analyzed by HPLC and GC-MS. The total content of anthocyanins was determined using the pH differential absorbance method and aliphatic acids by capillary electrophoresis. MicroLC-MS/MS was used for determination of cyanidin-3-glucoside (the predominant anthocyanin), 3,5-diglucoside, and 3-rutinoside, paeonidin-3-glucoside, 3,5-diglucoside, and 3-rutinoside, delphinidin-3-glucoside and 3-rutinoside, pelargonidin-3-glucoside, 3,5-diglucoside, and 3-rutinoside, quercetin, its 3-glucoside, and 3-rutinoside, epicatechin, protocatechuic, gentisic, ellagic, ferulic, caffeic, chlorogenic, and coumaric acids. The phenolic fraction displayed Folin-Ciocalteu reagent reducing (335 +/- 15 microg of gallic acid equivalent/mg) and DPPH and superoxide scavenging activity (IC50 12.1 +/- 0.1 and 115.5 +/- 6.4 microg/mL) and inhibited rat liver microsome peroxidation (IC50 160 +/- 20 microg/mL). The freeze-dried fruit and its phenolic fraction reduced the biofilm formation and adhesion to the artificial surface of Candida parapsilosis, Staphylococcus epidermidis, Escherichia coli, Enterococcus faecalis, and Streptococcus mutans.

• MeSH
• anthokyaniny chemie   MeSH
• antiinfekční látky farmakologie   MeSH
• antioxidancia farmakologie   MeSH
• Bacteria účinky léků   MeSH
• bakteriální adheze účinky léků   MeSH
• biofilmy účinky léků   MeSH
• fenoly farmakologie   MeSH
• houby fyziologie   účinky léků   MeSH
• jaterní mikrozomy metabolismus   účinky léků   MeSH
• Lonicera chemie   MeSH
• peroxidace lipidů účinky léků   MeSH
• rostlinné extrakty farmakologie   chemie   MeSH
• Publikační typ
• práce podpořená grantem MeSH

BACKGROUND: Lonicera caerulea L. (blueberry honeysuckle, Caprifoliaceae) is a traditional crop in northern Russia, China, and Japan. Its fruits are little known as edible berries in North America and Europe. This review deals with the botany and chemical composition of L. caerulea and the biological activity of its main constituents, focusing on the potential health benefits of the berries. METHODS AND RESULTS: PubMed, Science Direct and ISI Web of Knowledge databases were used for this paper. Literature sources include the period 1935-2007. L. caerulea berries a are rich source of phenolic compounds such as phenolic acids as well as anthocyanins, proanthocyanidins and other flavonoids, which display potential health promoting effects. Chemopreventive, antimicrobial, anti-adherence and antioxidant benefits, among others are described for these compounds. CONCLUSIONS: The potential of L. caerulea berries to prevent chronic diseases such as diabetes mellitus, cardiovascular diseases and cancer seems to be related above all to their phenolic content.

• MeSH
• fytoterapie MeSH
• Lonicera chemie   MeSH
• ovoce chemie   MeSH
• rostlinné extrakty chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH