A surface enhanced Raman scattering (SERS) spectrometry is an interesting alternative for a rapid molecular recognition of analytes at very low concentration levels. The hyphenation of this technique with advanced separation methods enhances its potential as a detection technique. Until now, it has been hyphenated mainly with common chromatographic and electrophoretic techniques. This work demonstrates for a first time a power of preparative isotachophoresis-surface enhanced Raman scattering spectrometry (pITP-SERS) combination on the analysis of model analyte (buserelin) in a complex biological sample (urine). An off-line identification of target analyte was performed using a comparison of Raman spectra of buserelin standard with spectra obtained by the analyses of the fractions from preparative isotachophoretic runs. SERS determination of buserelin was based on the method of standard addition to minimize the matrix effects. The linearity of developed method was obtained in the concentration range from 0.2 to 1.5 nmol L(-1) with coefficient of determination 0.991. The calculated limit of detection is in tens of pico mols per liter.

• MeSH
• buserelin moč   MeSH
• izotachoforéza metody   MeSH
• kovové nanočástice chemie   MeSH
• lidé MeSH
• Ramanova spektroskopie metody   MeSH
• senzitivita a specificita MeSH
• stříbro chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Klíčová slova
• DANOVAL, RELEFACT, SUPREFACT, ENANTONE-GYN,
• MeSH
• danazol MeSH
• gynekologie MeSH
• nafarelin MeSH
• Publikační typ
• přehledy MeSH

• Klíčová slova
• SUPREFACT NASAL SPRAY (HOECHST),
• MeSH
• chemická stimulace MeSH
• citráty MeSH
• dospělí MeSH
• fertilizace in vitro MeSH
• hormon uvolňující gonadotropiny analogy a deriváty   MeSH
• klomifen analogy a deriváty   MeSH
• lidé MeSH
• menotropiny MeSH
• ovarium účinky léků   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• srovnávací studie MeSH

• Klíčová slova
• ORIMETEN, NIZORAL, AGOSTILBEN, TURISTERON, PREMARIN, DIFOSTILBEN, HONVAN, TACE, ESTRACYT, SUPREFACT, ZOLADEX, LUCRIN, FLUCINOVIR, EULEXINPROSTANDRIL, CASODEX, ANDROCUR, PROSCAR, ANANDRON,
• MeSH
• androgeny MeSH
• farmakoterapie metody   MeSH
• hormony aplikace a dávkování   škodlivé účinky   terapeutické užití   MeSH
• karcinom farmakoterapie   sekundární   terapie   MeSH
• kastrace metody   MeSH
• lidé MeSH
• nádory prostaty farmakoterapie   terapie   MeSH
• orchiektomie MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH