cherry Dotaz Zobrazit nápovědu
Double-stranded RNA and total RNA purified from sour cherry leaves (Prunus cerasus, cv. Amarelka Chvalkovicka) was analyzed by high-throughput sequencing. BLAST annotation identified contigs with homology to several already known cherry-infecting viruses (prune dwarf virus, prunus necrotic ringspot virus, prunus virus F, little cherry virus 1) as well as contigs with sequences more distantly related to those of members of the family Betaflexiviridae and in particular to prunus virus T of the genus Tepovirus. The full genome sequence of a putative virus (6,847 nucleotides [nt]; GenBank no. MT090966) was assembled and completed at the genome ends. The genome has a typical tepovirus organization, containing three overlapping open reading frames (ORFs), encoding a replication-associated protein, a movement protein and a capsid protein, respectively. Both its genome organization and its phylogenetic relationships show that the virus belongs to the genus Tepovirus, but considering the species demarcation criteria for the family Betaflexiviridae, it appears to represent a novel virus species, and we propose the name "cherry virus T" (ChVT) for this virus.
- MeSH
- Flexiviridae klasifikace genetika izolace a purifikace MeSH
- fylogeneze MeSH
- genom virový * MeSH
- nemoci rostlin virologie MeSH
- otevřené čtecí rámce MeSH
- Prunus avium virologie MeSH
- sekvence nukleotidů MeSH
- sekvenování celého genomu MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- Publikační typ
- časopisecké články MeSH
Kazuistika intoxikace šestiletého dítěte toxiny bobkovišně lékařské obsaženými v jejích plodech a listech s celkembenigním průběhem spojeným pouze s gastrointestinálními příznaky a s úplným uzdravením. Autor varuje před stálečastějšími intoxikacemi u dětí i dospělých touto rostlinou a na nebezpečí, které hrozí při vážnějších intoxikacích.
Case report of the intoxication of a 6-year-old child with the toxins contained in the fruits and leaves of the laurel bayberry with a fairly benign course associated only with gastointestinal symptoms and complete recovery. The autorwarns of increasingly frequent intioxications in children and adults with plant and of the danger posed by more serious intoxications.
- Klíčová slova
- bobkovišeň lékařská,
- MeSH
- dítě MeSH
- jedovaté rostliny MeSH
- lidé MeSH
- otrava rostlinami * terapie MeSH
- slivoň toxicita MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The sweet cherry plant (Prunus avium L.) is primarily self-incompatible, with so-called S-alleles responsible for the inability of flowers to be pollinated not only by their own pollen grains but also by pollen from other cherries having the same S-alleles. This characteristic has wide-ranging impacts on commercial growing, harvesting, and breeding. However, mutations in S-alleles as well as changes in the expression of M locus-encoded glutathione-S-transferase (MGST) can lead to complete or partial self-compatibility, simplifying orchard management and reducing possible crop losses. Knowledge of S-alleles is important for growers and breeders, but current determination methods are challenging, requiring several PCR runs. Here we present a system for the identification of multiple S-alleles and MGST promoter variants in one-tube PCR, with subsequent fragment analysis on a capillary genetic analyzer. The assay was shown to unequivocally determine three MGST alleles, 14 self-incompatible S-alleles, and all three known self-compatible S-alleles (S3', S4', S5') in 55 combinations tested, and thus it is especially suitable for routine S-allele diagnostics and molecular marker-assisted breeding for self-compatible sweet cherries. In addition, we identified a previously unknown S-allele in the 'Techlovicka ́ genotype (S54) and a new variant of the MGST promoter with an 8-bp deletion in the ́Kronio ́ cultivar.
- MeSH
- alely MeSH
- polymerázová řetězová reakce MeSH
- Prunus avium * genetika MeSH
- šlechtění rostlin MeSH
- slivoň * genetika MeSH
- Publikační typ
- časopisecké články MeSH
The complete genomic sequence of a new virus from cherry trees was determined. Its genome is 5857 nt long and resembles that of members of the genus Luteovirus in its genomic organization and nucleotide sequence. Based on the species demarcation criteria for luteoviruses, the virus represents a new luteovirus species. Furthermore, a 47-nt-long inverted repeat was found at the 3' end of its genome. The virus has been provisionally named cherry-associated luteovirus (ChALV) and is the fourth member of the family Luteoviridae reported to naturally infect woody plants.
- MeSH
- 3' přiléhající oblast DNA MeSH
- délka genomu MeSH
- fylogeneze * MeSH
- genom virový * MeSH
- Luteovirus klasifikace genetika izolace a purifikace MeSH
- mapování chromozomů MeSH
- nemoci rostlin virologie MeSH
- obrácené repetice MeSH
- otevřené čtecí rámce MeSH
- RNA virová genetika MeSH
- sekvence nukleotidů MeSH
- slivoň švestka virologie MeSH
- Publikační typ
- časopisecké články MeSH
[1st ed.] xi, 80 s. : il.
- MeSH
- náhlé příhody MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- terapie
- urgentní lékařství
Certain light environments may hinder egg discrimination by hosts of foreign eggs, which could in some circumstances lead to the acceptance of non-mimetic eggs by hosts. We measured light parameters at red bishop (Euplectes orix) nests and used a model of avian visual processing to quantify the detectability of eggs in the light environment in which they are perceived. We found that the overall amount of light was very variable between red bishop nests and always sufficient for colour discrimination. A model of avian visual processing revealed that nest luminosity had no influence on host responses towards eggs which were painted dark brown. Dark eggs do not appear to be cryptic in red bishop nests and can be distinguished with ease, whereas natural red bishop eggs are usually accepted, despite the domed structure of the nest. We found little variation in both chromatic and achromatic contrasts between host and artificial eggs, indicating that there was very little variation in the light quality inside nests. We suggest that nest luminosity is likely to play a role in egg recognition in situations when light reaches threshold values for colour discrimination, i.e. in scotopic as opposed to photopic vision. Rejection rates for dark eggs were higher than for bright (conspecific) foreign eggs. More investigation of domed nest-building species is required, as this type of nest appears to have a highly variable light environment, dependent on both nest structure and habitat.
- MeSH
- barva MeSH
- druhová specificita MeSH
- ekosystém MeSH
- hnízdění fyziologie MeSH
- interakce hostitele a parazita fyziologie MeSH
- ovum * MeSH
- paraziti MeSH
- Passeriformes fyziologie MeSH
- rozpoznávání (psychologie) MeSH
- světlo * MeSH
- vaječná skořápka chemie MeSH
- zraková percepce fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
