different Fe content
Dotaz
Zobrazit nápovědu
Preparation of affinity polyacrylamide gels containing immobilized Fe(III) ions for the separation of proteins exhibiting metal ion binding properties is described. The presented method enables uniform distribution of immobilized metal ions in the affinity part of the polyacrylamide separating gel. Affinity gels prepared by this way are suitable to follow the effect of different concentrations of metal ions immobilized in polyacrylamide gel on a protein electrophoretic behavior. Polyacrylamide gels containing immobilized Fe(III) ions were used to study the electrophoretic behavior of two model proteins differing in their phosphate group content: chicken ovalbumin and bovine α-casein. For the electrophoretic separation, both the native and the denaturating conditions were used.
Influence of in vitro temperature on sperm antioxidant enzyme activity, thiobarbituric acid-reactive substance (TBARS) content and motility parameters was evaluated in sterlet Acipenser ruthenus and rainbow trout Oncorhynchus mykiss. Sperm activation was conducted at 4, 14 and 24 °C in both species. Duration of motility was significantly longer at 4 °C than at 14 and 24 °C in both species. At 60 s post-activation, the velocity of sterlet spermatozoa was highest at 24 °C. This trend continued to 420 s post-activation. In rainbow trout, at 10 s post-activation, the highest velocity was observed at 14 °C. Significantly higher catalase activity was seen at 4 °C in both species. No significant difference in spermatozoon superoxide dismutase activity among temperatures was observed. In sterlet, TBARS content was significantly higher at 24 °C compared to other temperatures, but, in rainbow trout, it was highest at 4 °C. The results presume species-specific level of antioxidant enzyme activity and TBARS content at studied temperatures.
- MeSH
- antioxidancia metabolismus MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- motilita spermií * MeSH
- Oncorhynchus mykiss fyziologie MeSH
- peroxidace lipidů MeSH
- ryby fyziologie MeSH
- spermie enzymologie MeSH
- superoxiddismutasa metabolismus MeSH
- teplota * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVES: Overweight and obesity are risk factors for many diseases, nutrition leading to these phenomena is not only a question of disbalance between energy intake and expenditure, but also the presence of micronutrients. In our study, we focused on measuring residues of chromium, zinc and iron in the hair of men with different BMI. METHODS: Hair samples and anthropometric questionnaires were collected from 45 males. Numbers of subjects and age structure were comparable between the three BMI groups. The determination of metal levels was performed by inductively coupled plasma mass spectrometry after mineralization of the hair. RESULTS: The hair of obese men contained significantly higher chromium (0.096 μg/g vs. 0.045 μg/g, p = 0.0039) and iron (9.42 μg/g vs. 5.84 μg/g, p = 0.0009) concentrations than that of overweight men, but no significant difference between the normal-weight group and the obese group were found. The concentration of zinc was lower in obese subjects compared to overweight subjects (183.5 μg/g vs. 206.2 μg/g, p = 0.038). Also, statistically significant correlations between chromium and iron concentrations in hair and BMI were found (r = 0.307, p = 0.040, r = 0.360, p = 0.015, respectively). According to our results, age did not significantly affect chromium, iron and zinc concentrations in hair. CONCLUSION: Consistent with some published studies, we have found that obese men have higher chromium and iron concentrations and lower zinc concentrations in hair.
- MeSH
- chrom MeSH
- lidé MeSH
- nadváha * MeSH
- obezita epidemiologie MeSH
- vlasy, chlupy * MeSH
- zinek MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Historical slags from the past Fe and Cu-Co production were investigated in order to evaluate either their potential for utilization or their long-term environmental risk for unsupervised old smelting areas. Here, we studied ferrous slags produced during the recovery of Fe from siderite-Cu ores in Slovakia and two different types of non-ferrous slags produced during the recovery of Cu and Co from Kupferschiefer ores in Germany. The glassy character, rare occurrence of primary silicate phases, and the lack of secondary phases in Cu slags indicate their stability for a prolonged period of time. Electron microprobe analytical work showed that the metals and metalloids (Cu, Co, Fe, Zn, Pb, As) are largely encased in droplets of matte and metal alloys and remain protected by the glassy matrix with its low weathering rate. Fe and Co slags are composed of high-temperature silicates such as wollastonite, cristobalite, as well as olivine, feldspar, quartz, leucite, pyroxene, and pyroxenoids. The presence of secondary phases attests to a certain degree metal release owing to weathering. Assuming minimal contents of metals in slags after a treatment with dilute H2SO4, slags could be used as pozzolanas for addition to cement.
- MeSH
- hodnocení rizik MeSH
- kovy analýza MeSH
- látky znečišťující půdu analýza MeSH
- monitorování životního prostředí MeSH
- nakládání s odpady * MeSH
- recyklace * MeSH
- životní prostředí * MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Německo MeSH
- Slovenská republika MeSH
An amorphous Mn oxide (AMO), nanomaghemite, and nanomagnetite were used as potential amendments reducing the mobility of As in three contrasting contaminated soils differing in origin of As contamination. Adsorption experiments and XPS analyses combined with incubation batch experiments and pH-static leaching tests were used. The AMO showed excellent adsorption capacity for As(V) reaching a maximum of 1.79 mmol g(-1) at pH 7 and 8. Interestingly, the adsorption capacity in this case decreases with decreasing pH, probably as a result of AMO dissolution at lower pH values. Chemical sorption of As(V) onto AMO was further confirmed with XPS. Both Fe nano-oxides proved the highest adsorption capacity at pH 4 reaching 11 mg g(-1) of adsorbed As(V). The AMO was also the most efficient amendment for decreasing As concentrations in soil solutions during 8 weeks of incubation. Additionally, pH-static leaching tests were performed at pH 4, 5, 6, 7, and natural pH (not adjusted) and AMO again proved the highest ability to decrease As content in leachate. On the other hand, strong dissolution of this amendment at lower pH values (especially pH 4) was observed. For that reason, AMO appears as a promising stabilizing agent for As, especially in neutral, alkaline, or slightly acidic soils, where As(V) species are expected to be more mobile.
- MeSH
- adsorpce MeSH
- arsen analýza chemie MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- látky znečišťující půdu analýza chemie MeSH
- nanočástice chemie MeSH
- oxidy chemie MeSH
- půda chemie MeSH
- regenerace a remediace životního prostředí MeSH
- sloučeniny manganu chemie MeSH
- železité sloučeniny chemie MeSH
- znečištění životního prostředí analýza MeSH
- Publikační typ
- časopisecké články MeSH
Accumulation of selected heavy metals (Cd, Pb, Ni, Cr, Fe, and Zn) and phenolic metabolites (total soluble phenols, cichoric and caftaric acid) in dandelion organs (leaves, roots, inflorescences/anthodia) collected from six localities within the industrial town Košice (eastern Slovakia) were studied. Localities from the vicinity of a steel factory (Cd, Fe) and heavy traffic (Pb, Ni, Cr, Zn) contained the highest amount of individual metals in the soil but a significant correlation between soil and organ metal content was found only for Cr in the leaves (r(2) = 0.7679). The amount of Cd and partially Pb differed among localities in all organs and especially in the leaves and anthodia, indicating probably the impact of atmospheric pollution. The bioaccumulation factor was <1 for almost all metals, suggesting that given dandelion species is not metal accumulator. Translocation factor did not reach values close to or over 1 only for Cd, indicating a root-to-shoot movement of Pb, Ni and Zn though the impact of air pollution on leaves cannot be excluded. A strong correlation between leaf Cd and leaf total phenols, cichoric and caftaric acids was observed (r(2) = 0.7926, 0.8682 and 0.8830, respectively), indicating that phenolic metabolites act in the protection of dandelion against Cd excess. Overall, our data indicate low pollution of urban soil by Cd (5.53-113.8 ng g(-1)) and partially by Cr and the suitability of above-ground organs of dandelion species for the monitoring of air pollution mainly by Cd.
- MeSH
- látky znečišťující půdu analýza chemie MeSH
- látky znečišťující vzduch analýza chemie MeSH
- lidé MeSH
- listy rostlin chemie MeSH
- monitorování životního prostředí * MeSH
- půda chemie MeSH
- Taraxacum chemie MeSH
- těžké kovy analýza chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Slovenská republika epidemiologie MeSH
Dunaliella salina is a rich source of carotenoids. Carotenoid production is induced under specific conditions, i.e., high light intensity, high salt concentration, nutrient limitation, and suboptimal temperatures in this microalga. The control of environmental factors is vital for high productivity of carotenoids. In this paper, the effect of different ethanol concentrations in combination with nitrogen deficiency was investigated to induce carotenoid production in D. salina CCAP 19/18. Also, some biochemical and molecular parameters were investigated in response to ethanol in the cells. It was shown that ethanol at 0.5% concentration increased cell number but, at 5% concentration, reduced cell viability compared to the control. The highest carotenoid production was achieved at 3% ethanol concentration, which was 1.46 fold higher than the nitrogen deficiency condition. Investigation of the 3 carotenoid biosynthesis genes revealed that their expression levels increased at 3% ethanol concentration, and the phytoene synthase gene was the most upregulated one. Lipid peroxidation increased at both 3% and 5% ethanol concentrations. At 3% concentration, the activity of catalase and superoxide dismutase increased, but no significant changes were seen at 5% ethanol concentration. Peroxidase activity reduced at both 3% and 5% concentrations. Moreover, proline and reducing sugar content increased at 3% concentration while decreased at 5% ethanol concertation. The results showed that at 3% ethanol concentration, higher carotenoid productivity was associated with an increase in other intracellular responses (molecular and biochemical). Ethanol as a controllable element may be beneficial to increase carotenoid production even under inappropriate environmental conditions in D. salina.
- MeSH
- antioxidancia * MeSH
- dusík MeSH
- karotenoidy * metabolismus MeSH
- superoxiddismutasa metabolismus MeSH
- světlo MeSH
- Publikační typ
- časopisecké články MeSH
Aspergillus oryzae G15 was cultured on Czapek yeast extract agar medium containing different concentrations of copper and lead to investigate the mechanisms sustaining metal tolerance. The effects of heavy metals on biomass, metal accumulation, metallothionein (MT), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were evaluated. Cu and Pb treatment remarkably delayed sclerotial maturation and inhibited mycelial growth, indicating the toxic effects of the metals. Cu decreased sclerotial biomass, whereas Pb led to an increase in sclerotial biomass. G15 bioadsorbed most Cu and Pb ions on the cell surface, revealing the involvement of the extracellular mechanism. Cu treatment significantly elevated MT level in mycelia, and Pb treatment at concentrations of 50-100 mg/L also caused an increase in MT content in mycelia. Both metals significantly increased MDA level in sclerotia. The variations in MT and MDA levels revealed the appearance of heavy metal-induced oxidative stress. The activities of SOD, CAT, and POD varied with heavy metal concentrations, which demonstrated that tolerance of G15 to Cu and Pb was associated with an efficient antioxidant defense system. In sum, the santioxidative detoxification system allowed the strain to survive in high concentrations of Cu and Pb. G15 depended mostly on sclerotial differentiation to defend against Pb stress.
- MeSH
- Aspergillus oryzae účinky léků genetika růst a vývoj metabolismus MeSH
- fungální proteiny genetika metabolismus MeSH
- katalasa genetika metabolismus MeSH
- malondialdehyd metabolismus MeSH
- měď farmakologie MeSH
- mycelium účinky léků genetika růst a vývoj metabolismus MeSH
- olovo farmakologie MeSH
- oxidační stres účinky léků MeSH
- superoxiddismutasa genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Mid-exponential cultures of two traditional biotechnological yeast species, winery Saccharomyces cerevisiae and the less ethanol tolerant bottom-fermenting brewery Saccharomyces pastorianus, were exposed to different concentrations of added ethanol (3, 5 and 8%) The degree of ethanol-induced cell stress was assessed by measuring the cellular activity of superoxide dismutase (SOD), level of lipid peroxidation products, changes in cell lipid content and fatty acid profile. The resveratrol as an antioxidant was found to decrease the ethanol-induced rise of SOD activity and suppress the ethanol-induced decrease in cell lipids. A lower resveratrol concentration (0.5 mg/l) even reduced the extent of lipid peroxidation in cells. Resveratrol also alleviated ethanol-induced changes in cell lipid composition in both species by strongly enhancing the proportion of saturated fatty acids and contributing thereby to membrane stabilization. Lower resveratrol concentrations could thus diminish the negative effects of ethanol stress on yeast cells and improve their physiological state. These effects may be utilized to enhance yeast vitality in high-ethanol-producing fermentations or to increase the number of yeast generations in brewery.
- MeSH
- antioxidancia metabolismus MeSH
- ethanol farmakologie MeSH
- fyziologický stres účinky léků MeSH
- lipidy fyziologie MeSH
- mastné kyseliny metabolismus MeSH
- metabolismus lipidů účinky léků MeSH
- peroxidace lipidů účinky léků MeSH
- Saccharomyces cerevisiae účinky léků MeSH
- stilbeny farmakologie MeSH
- superoxiddismutasa metabolismus MeSH
- víno mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
Understanding the response of a crop to drought is the first step in the breeding of tolerant genotypes. In our study, two maize (Zea mays L.) genotypes with contrasting sensitivity to dehydration were subjected to moderate drought conditions. The subsequent analysis of their physiological parameters revealed a decreased stomatal conductance accompanied by a slighter decrease in the relative water content in the sensitive genotype. In contrast, the tolerant genotype maintained open stomata and active photosynthesis, even under dehydration conditions. Drought-induced changes in the leaf proteome were analyzed by two independent approaches, 2D gel electrophoresis and iTRAQ analysis, which provided compatible but only partially overlapping results. Drought caused the up-regulation of protective and stress-related proteins (mainly chaperones and dehydrins) in both genotypes. The differences in the levels of various detoxification proteins corresponded well with the observed changes in the activities of antioxidant enzymes. The number and levels of up-regulated protective proteins were generally lower in the sensitive genotype, implying a reduced level of proteosynthesis, which was also indicated by specific changes in the components of the translation machinery. Based on these results, we propose that the hypersensitive early stomatal closure in the sensitive genotype leads to the inhibition of photosynthesis and, subsequently, to a less efficient synthesis of the protective/detoxification proteins that are associated with drought tolerance.
- MeSH
- 2D gelová elektroforéza MeSH
- antioxidancia metabolismus MeSH
- dehydratace MeSH
- fyziologická adaptace MeSH
- genotyp MeSH
- glutathionreduktasa metabolismus MeSH
- katalasa metabolismus MeSH
- kukuřice setá enzymologie genetika fyziologie MeSH
- období sucha MeSH
- proteomika MeSH
- průduchy rostlin fyziologie MeSH
- superoxiddismutasa metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
