polycarbonate OR C005506 Dotaz Zobrazit nápovědu
The contact of blood with artificial materials generally leads to immediate protein adsorption (fouling), which mediates subsequent biological processes such as platelet adhesion and activation leading to thrombosis. Recent progress in the preparation of surfaces able to prevent protein fouling offers a potential avenue to mitigate this undesirable effect. In the present contribution, we have prepared several types of state-of-the-art antifouling polymer brushes on polycarbonate plastic substrate, and investigated their ability to prevent platelet adhesion and thrombus formation under dynamic flow conditions using human blood. Moreover, we compared the ability of such brushes--grafted on quartz via an adlayer analogous to that used on polycarbonate--to prevent protein adsorption from human blood plasma, assessed for the first time by means of an ultrahigh frequency acoustic wave sensor. Results show that the prevention of such a phenomenon constitutes one promising route toward enhanced resistance to thrombus formation, and suggest that antifouling polymer brushes could be of service in biomedical applications requiring extensive blood-material surface contact.
- MeSH
- adhezivita trombocytů účinky léků MeSH
- křemen chemie MeSH
- lidé MeSH
- polykarboxylátový cement chemie farmakologie MeSH
- povrchové vlastnosti * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Different models of phospholipid membranes (PLMs) have been utilized for better understanding of properties and functionality of real biological membranes. Mechanisms of transport processes and membrane properties can be investigated on planar phospholipid bilayers. Electrochemical behavior of phosphatidylcholine-cholesterol mixtures in the PLM form was studied by electrochemical impedance spectroscopy. Phosphatidylcholine (lecithin) and cholesterol are essential for living cells and, therefore, they were chosen for experiments. Different lecithin/cholesterol ratios were tested on different polycarbonate(PC). This material is formed by a condensation polyme¬rization resulting in a carbon that is bonded to three oxygens. The time dependence of PLM formation in the presence of cholesterol was investigated.
- MeSH
- 1,2-dipalmitoylfosfatidylcholin chemie MeSH
- buněčná membrána MeSH
- cholesterol chemie MeSH
- elektrochemické techniky metody přístrojové vybavení využití MeSH
- elektrochemie * MeSH
- fluidita membrány MeSH
- impedanční spektroskopie * metody přístrojové vybavení využití MeSH
- lecitiny chemie MeSH
- lipidové dvojvrstvy * MeSH
- Publikační typ
- práce podpořená grantem MeSH
Development of biomaterials for hernia and pelvic organ prolapse (POP) repair is encouraged because of high local complication rates with current materials. Therefore, we aimed to develop a functionalized electrospun mesh that promotes tissue ingrowth and provides adequate mechanical strength and compliance during degradation. We describe the in vivo function of a new supramolecular bioactivated polycarbonate (PC) material based on fourfold hydrogen bonding ureidopyrimidinone (UPy) units (UPy-PC). The UPy-PC material was functionalized with UPy-modified cyclic arginine-glycine-aspartic acid (cRGD) peptide additives. Morphometric analysis of the musculofascial content during wound healing showed that cRGD functionalization promotes myogenesis with inhibition of collagen deposition at 14 days. It also prevents muscle atrophy at 90 days and exerts an immunomodulatory effect on infiltrating macrophages at 14 days and foreign body giant cell formation at 14 and 90 days. Additionally, the bioactivated material promotes neovascularization and connective tissue ingrowth. Supramolecular cRGD-bioactivation of UPy-PC-meshes promotes integration of the implant, accelerates tissue ingrowth and reduces scar formation, resulting in physiological neotissue formation when used for abdominal wall reconstruction in the rat hernia model. Moreover, cRGD-bioactivation prevents muscle atrophy and modulates the inflammatory response. Our results provide a promising outlook towards a new type of biomaterial for the treatment of hernia and POP. STATEMENT OF SIGNIFICANCE: Development of biomaterials for hernia and pelvic organ prolapse (POP) repair is encouraged because of high local complication rates with current materials. Ureidopyrimidinone-polycarbonate is a elastomeric and biodegradable electrospun mesh, which could mimic physiological compliance. The UPy-PC material was functionalized with UPy-modified cyclic arginine-glycine-aspartic acid (cRGD) peptide additives. Supramolecular cRGD-bioactivation of UPy-PC-meshes promotes integration of the implant, accelerates tissue ingrowth and reduces scar formation, resulting in physiological neotissue formation when used for abdominal wall reconstruction in rat hernia model. Moreover, cRGD-bioactivation prevents muscle atrophy and modulates the inflammatory response. These data provide a promising outlook towards a new type of biomaterial for the treatment of hernia and POP.
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- břišní stěna chirurgie MeSH
- chirurgické síťky * MeSH
- chrupavka metabolismus MeSH
- cyklické peptidy chemie farmakologie MeSH
- granulom prevence a kontrola MeSH
- polykarboxylátový cement chemie farmakologie MeSH
- potkani Sprague-Dawley MeSH
- pyrimidinony chemie farmakologie MeSH
- svalová atrofie prevence a kontrola MeSH
- vývoj svalů účinky léků MeSH
- zánět prevence a kontrola MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of polycarbonate plastics and epoxy resins and thus can be found in various plastic products and thermal papers. The microbiota disrupting effect of BPS on the community structure of the microbiome has already been reported, but little is known on how BPS affects bacterial activity and function. To analyze these effects, we cultivated the simplified human intestinal microbiota (SIHUMIx) in bioreactors at a concentration of 45 µM BPS. By determining biomass, growth of SIHUMIx was followed but no differences during BPS exposure were observed. To validate if the membrane composition was affected, fatty acid methyl esters (FAMEs) profiles were compared. Changes in the individual membrane fatty acid composition could not been described; however, the saturation level of the membranes slightly increased during BPS exposure. By applying targeted metabolomics to quantify short-chain fatty acids (SCFA), it was shown that the activity of SIHUMIx was unaffected. Metaproteomics revealed temporal effect on the community structure and function, showing that BPS has minor effects on the structure or functionality of SIHUMIx.
- Publikační typ
- časopisecké články MeSH
A wide range of substances currently present in the environment may interact with biologically important macromolecules and may thus negatively affect their function. One of them, bisphenol A (BPA), is a basic material used in the production of polycarbonate plastics and epoxy resins, which adds flexibility and durability to the products. Because of its ability to migrate from these products, it represents a potential risk to the environment and to the quality of life of the organisms living there. One of the known mechanisms of BPA's action on organisms acts via estrogen receptors, leading to the blocking or mimicking of natural ligands, i.e., sex hormones. Another way BPA can affect cells is to induce oxidative damage to macromolecules including DNA. Therefore, BPA is referred to as a genotoxic agent. Moreover, BPA can also act epigenetically, through altered methylation of some genes, which may affect their expression. This process may represent a risk of inducing or promoting the transformation of healthy cells into tumorous ones. Such disruption of the body's natural environment is therefore undesirable and in contradiction with a healthy and prosperous individual's life.
- Klíčová slova
- bisfenol,
- MeSH
- buněčné dělení účinky léků MeSH
- endokrinní disruptory chemie škodlivé účinky MeSH
- exprese genu účinky léků MeSH
- fenoly * chemie škodlivé účinky MeSH
- lidé MeSH
- nádory etiologie genetika MeSH
- poškození DNA MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Liposomes are one of the most important drug delivery vectors, nowadays used in clinics. In general, polyethylene glycol (PEG) is used to ensure the stealth properties of the liposomes. Here, we have employed hydrophilic, biocompatible and highly non-fouling N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers containing hydrophobic cholesterol anchors for the surface modification of liposomes, which were prepared by the method of lipid film hydration and extrusion through 100 nm polycarbonate filters. Efficient surface modification of liposomes was confirmed by transmission electron microscopy, atomic force microscopy, and gradient ultracentrifugation. The ability of long-term circulation in the vascular bed was demonstrated in rabbits after i.v. application of fluorescently labelled liposomes. Compared to PEGylated liposomes, HPMA-based copolymer-modified liposomes did not induce specific antibody formation and did not activate murine and human complement. Compared with PEGylated liposomes, HPMA-based copolymer-modified liposomes showed a better long-circulating effect after repeated administration. HPMA-based copolymer-modified liposomes thus represent suitable new candidates for a generation of safer and improved liposomal drug delivery platforms.
- MeSH
- akrylamidy chemie MeSH
- aktivace komplementu účinky léků MeSH
- cholesterol chemie krev MeSH
- hydrofobní a hydrofilní interakce * MeSH
- králíci MeSH
- lidé MeSH
- liposomy * MeSH
- myši MeSH
- polyethylenglykoly * chemie MeSH
- polymery chemie MeSH
- povrchové vlastnosti * MeSH
- systémy cílené aplikace léků MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Age-related macular degeneration (AMD) is the most frequent cause of blindness in developed countries. The replacement of dysfunctional human retinal pigment epithelium (hRPE) cells by the transplantation of in vitro-cultivated hRPE cells to the affected area emerges as a feasible strategy for regenerative therapy. Synthetic biomimetic membranes arise as powerful hRPE cell carriers, but as biodegradability is a requirement, it also poses a challenge due to its limited durability. hRPE cells exhibit several characteristics that putatively respond to the type of membrane carrier, and they can be used as biomarkers to evaluate and further optimize such membranes. Here, we analyze the pigmentation, transepithelial resistance, genome integrity, and maturation markers of hRPE cells plated on commercial polycarbonate (PC) versus in-house electrospun polylactide-based (PLA) membranes, both enabling separate apical/basolateral compartments. Our results show that PLA is superior to PC-based membranes for the cultivation of hRPEs, and the BEST1/RPE65 maturation markers emerge as the best biomarkers for addressing the quality of hRPE cultivated in vitro. The stability of the cultures was observed to be affected by PLA aging, which is an effect that could be partially palliated by the coating of the PLA membranes.
- Publikační typ
- časopisecké články MeSH
alpha-Tocopheryl succinate (alpha-TOS) is a semisynthetic analogue of alpha-tocopherol with selective toxicity to the cancer cells and anticancer activity in vivo. Yet, no suitable formulation of alpha-TOS for medical application has been reported. Various formulations, for example, solutions in organic solvents, oil emulsions and vesicules prepared by spontaneous vesiculation, polyethylene glycol conjugates and liposomes of various compositions have been tested. We developed and characterised a stable lyophilised liposome-based alpha-TOS formulation. alpha-TOS (15 mol%) was incorporated into large oligolamellar vesicles (OLVs) composed of soy phosphatidylcholine (SPC) by the method of lipid film hydration followed by extrusion through polycarbonate filters. Stabilised liposomal formulation was prepared by lyophilisation in the presence of sucrose (molar ratio lipid/sucrose, 1:5). The size distribution of the liposomes (130-140 nm, polydispersity index 0.14) as well as the stable lipid and alpha-TOS contents were preserved during storage in the lyophilised form at 2-8 degrees C for at least 6 months. The data indicate good physical and chemical stability of the lyophilised preparation of alpha-TOS liposomes that can be used in clinical medicine.
- MeSH
- alfa-tokoferol aplikace a dávkování chemie MeSH
- antitumorózní látky aplikace a dávkování chemie MeSH
- lipidy chemie MeSH
- liposomy MeSH
- lyofilizace MeSH
- lysofosfolipidy chemie MeSH
- peroxid vodíku chemie MeSH
- povrchové vlastnosti MeSH
- příprava léků metody MeSH
- skladování léků MeSH
- stabilita léku MeSH
- transmisní elektronová mikroskopie MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
