• MeSH
• antigeny bakteriální izolace a purifikace   MeSH
• dechové testy metody   MeSH
• ELISA metody   využití   MeSH
• Helicobacter pylori imunologie   izolace a purifikace   MeSH
• infekce vyvolané Helicobacter pylori imunologie   patologie   terapie   MeSH
• lidé MeSH
• senzitivita a specificita MeSH
• ureasa diagnostické užití   MeSH
• výběr pacientů MeSH
• Check Tag
• lidé MeSH

Differentiation of the oxidase positive staphylococci, Staphylococcus sciuri, Staphylococcus lentus, Staphylococcus vitulinus and Staphylococcus fleurettii, based on tributyrin, urease, caseinase, gelatinase and DNase activity is described. These tests may be used for preliminary identification of oxidase positive isolates of staphylococci resulting in more accurate identification of these species.

• MeSH
• deoxyribonukleasy metabolismus   MeSH
• druhová specificita MeSH
• lidé MeSH
• metaloendopeptidasy metabolismus   MeSH
• mikrobiologie životního prostředí MeSH
• oxidoreduktasy metabolismus   MeSH
• potravinářská mikrobiologie MeSH
• senzitivita a specificita MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus klasifikace   enzymologie   MeSH
• techniky typizace bakterií metody   MeSH
• triglyceridy metabolismus   MeSH
• ureasa metabolismus   MeSH
• želatinasy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Jugoslávie MeSH

Enterococci isolated from 28 different commercially available feeds (10-1000 CFU/mL) were identified and their probiotic potential was determined. Species identification of 22 selected strains was performed by intergenic length-polymorphism analysis (tRNA-PCR); PCR products were analyzed using capillary electrophoresis. Six strains were allotted to the species Enterococcus faecium, four to E. faecalis, one to E. hirae; the remaining strains were not classed. The strains were sensitive to vancomycin, ampicillin, tetracycline and rifampicin. They were able to adhere to human as well as canine intestinal mucus. They produced lactic acid (0.99-1.04 mmol/L) and most of them were urease-positive with sufficient survival in 5 % Oxgall-bile. They did not show any inhibitory activity due to antimicrobial substances. Plasmid DNA was detected in 8 strains, the bands responding to small molecular size (10 kbp). Considering all probiotically important properties, E. faecium strain EE3 was suggested as potential feed additive.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální proteiny metabolismus   MeSH
• Enterococcus chemie   izolace a purifikace   metabolismus   účinky léků   MeSH
• krmivo pro zvířata mikrobiologie   MeSH
• kyselina mléčná metabolismus   MeSH
• lidé MeSH
• probiotika chemie   izolace a purifikace   MeSH
• psi MeSH
• střeva mikrobiologie   MeSH
• ureasa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• psi MeSH
• zvířata MeSH

A simple nucleic acid amplification test (NAAT) was developed for detection of Ureaplasma urealyticum infection based on the PCR amplification of the urease gene (UU1/UU2 Test). DNA was extracted from urogenital swabs and a 225-bp long DNA fragment was amplified by PCR. NAAT was compared to the commercial amplification kit for sexually transmitted disease reference assay. The sensitivity and specificity of the UU1/UU2 Test were determined to be 100 and 98.9%, respectively. The overall prevalence rate in this group of patients was found to be about 236 per 1000 (283 and 166 per 1000 in females and males, respectively). These data demonstrate that UU1/UU2 Test is suitable for effective epidemiological screening and/or diagnostic practice.

• MeSH
• finanční podpora výzkumu jako téma MeSH
• lidé MeSH
• Mycoplasmataceae genetika   MeSH
• polymerázová řetězová reakce metody   využití   MeSH
• sexuálně přenosné nemoci bakteriální diagnóza   MeSH
• techniky amplifikace nukleových kyselin metody   využití   MeSH
• Ureaplasma urealyticum genetika   izolace a purifikace   MeSH
• ureaplasmatické infekce diagnóza   MeSH
• ureasa genetika   MeSH
• vaginální stěr metody   využití   MeSH
• Check Tag
• lidé MeSH