OBJECTIVE: The aim of our study was to investigate the relationship between alcohol-related mortality in Slovak regions, as represented by Nomenclature of Territorial Units for Statistics (NUTS) III level. METHODS: We used data from mortality reports spanning 1996-2017 in the Slovak Republic. Data was provided by the National Health Information Centre in Slovakia. We applied two-dimensional correspondence analysis where the dimensions are regions and selected alcohol-related deaths diagnoses are classified by the International Classification of Diseases. RESULTS: Analysis revealed a relationship between the Prešov region and diagnoses I42 - Cardiomyopathy and K29 - Alcoholic gastritis. Furthermore, the Banská Bystrica and Žilina regions correspond to G31 - Degeneration of nervous system due to alcohol and K86 - Alcohol-induced chronic pancreatitis. In the case of K70 - Alcoholic liver disease - the Banská Bystrica, Trenčín and Nitra regions are identified as regions which have an intermediate relationship with this diagnosis. The Trnava region corresponds to F10 - Acute alcohol intoxication. The Trenčín and Nitra regions correspond closely to G62 - Alcoholic polyneuropathy. Perfect correspondence can be seen between the Košice region and K73 - Chronic hepatitis, not elsewhere classified. K74 - Fibrosis and cirrhosis of liver diagnosis also corresponds with the Košice region. CONCLUSIONS: The results of this analysis provide valuable insights for national and regional health policymakers in the process of preparing high-quality health regional plans, as well as retrospectively assessing the success of existing health policies and interventions in this area. Facts presented in the study justify the need for specialised health care, which is part of the process of building an Integrated Health Care Centre in Slovakia.

• Klíčová slova
• Slovakia, alcohol-related deaths, correspondence analysis, region,
• MeSH
• lidé MeSH
• mortalita trendy   MeSH
• poruchy způsobené alkoholem mortalita   MeSH
• prostorová analýza MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Slovenská republika epidemiologie   MeSH

Understanding spatial distribution of organisms in heterogeneous environment remains one of the chief issues in ecology. Spatial organization of freshwater fish was investigated predominantly on large-scale, neglecting important local conditions and ecological processes. However, small-scale processes are of an essential importance for individual habitat preferences and hence structuring trophic cascades and species coexistence. In this work, we analysed the real-time spatial distribution of pelagic freshwater fish in the Římov Reservoir (Czechia) observed by hydroacoustics in relation to important environmental predictors during 48 hours at 3-h interval. Effect of diurnal cycle was revealed of highest significance in all spatial models with inverse trends between fish distribution and predictors in day and night in general. Our findings highlighted daytime pelagic fish distribution as highly aggregated, with general fish preferences for central, deep and highly illuminated areas, whereas nighttime distribution was more disperse and fish preferred nearshore steep sloped areas with higher depth. This turnover suggests prominent movements of significant part of fish assemblage between pelagic and nearshore areas on a diel basis. In conclusion, hydroacoustics, GIS and spatial modelling proved as valuable tool for predicting local fish distribution and elucidate its drivers, which has far reaching implications for understanding freshwater ecosystem functioning.

• MeSH
• akustika MeSH
• ekosystém MeSH
• geografické informační systémy MeSH
• monitorování životního prostředí MeSH
• populační dynamika MeSH
• prostorová analýza * MeSH
• ryby fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

During the reign of the first Ptolemaic kings in Egypt, mainly in the 3rd and 2nd centuries BCE, the Egyptian cults related to the divine couple of Isis and Sarapis (i.e. the Isiac cults) spread successfully from Egypt to ports and coastal cities of the ancient Mediterranean. The discussion on the topic of the factors involved in the process of the early spread of these cults outside Egypt is still open and, so far, the research in this area has been conducted mainly by using established historiographical methods. However, these methods are limited when dealing with the interplay among different variables involved in complex historical processes. This article aims to overcome these limits by using a quantitative spatial network analysis. The results of our previous published research, which focused on a quantitative evaluation of the impact of individual factors on the early spread of the Isiac cults across the ancient Aegean Islands, suggest that the process was promoted by military and commercial activities of the Ptolemaic dynasty, and that the Ptolemaic military operations were the most influential factor. Following these results, this article focuses on the early spread of the Isiac cults on the west coast of Hellenistic Asia Minor, i.e. the region which the Ptolemies attempted to control in the 3rd and 2nd centuries BCE. The statistically significant results presented in this article support the hypothesis that the Ptolemaic political engagement in Asia Minor had a positive impact on the early spread of the Isiac cults. The results also suggest that the activities of the Seleucid dynasty, a political rival of the Ptolemies, in the area of interest could have constituted an immunological factor limiting the spread of the Isiac cults further to the eastern parts of Asia Minor.

• MeSH
• archeologie MeSH
• dějiny starověku MeSH
• doprava MeSH
• lidé MeSH
• politika * MeSH
• prostorová analýza MeSH
• Check Tag
• dějiny starověku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Asie MeSH
• starověký Egypt MeSH

Cancer cells depend on nucleotides for proliferation. Inhibition of nucleotide metabolism by antimetabolites is a well-established anticancer therapy. However, resistance and toxicity to antimetabolite treatments reduce their effectiveness. Here, we focus on the pyrimidine de novo synthesis pathway, which is crucial for cancer cell proliferation, yet its pharmacological targeting in cancer has been without much clinical success so far. Hence, it is important to understand how cancer cells cope with the insufficiency of this pathway. Here, we describe a procedure to prepare subcutaneous tumor model deficient in de novo pyrimidine synthesis. For examination of metabolic responses to de novo synthesis blockade in tumors, we propose application of MALDI imaging that allows spatially resolved examination of metabolic responses to de novo synthesis blockade in tumors.

• Klíčová slova
• CRISPR/Cas9, Cancer, MALDI, Metabolism, Nucleotides, Pyrimidines,
• MeSH
• lidé MeSH
• nádory * metabolismus   MeSH
• nukleotidy MeSH
• prostorová analýza MeSH
• pyrimidiny MeSH
• sekvence CRISPR * MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• nukleotidy MeSH
• pyrimidiny MeSH

The sustenance of humans and livestock depends on the protection of the soil. Consequently, the pollution of the soil with potentially toxic elements (PTEs) is of great concern to humanity. The objective of this study is to investigate the source apportionment, concentration levels and spatial distribution of PTEs in selected soils in Frýdek-Místek District of the Czech Republic. The total number of soil samples was 70 (topsoil 49 and 21 subsoils) and was analysed using a portable XRF machine. Contamination factor and the pollution index load were used for the assessment and interpreting the pollution and distribution of PTEs in the soils. The inverse distance weighting was used for the spatial evaluation of the PTEs. The results of the analysis showed that the area is composed of low-to-high pollution site. PTEs displayed spatial variation patterns. The average PTE concentration decreases in this Fe > Ti > Ba > Zr > Rb > Sr > Cr > Y>Cu > Ni > Th order for the topsoil and also decreases in this Fe > Ti > Zr > Ba > Rb > Sr > Cr > Y > Cu > Ni > and Th order for the subsoil. These PTEs Cr, Ni, Cu, Rb, Y, Zr, Ba, Th, and Fe were far above the baseline European average value and the World average value level, respectively. The source apportionment showed the dominance of Cr, Ni, Rb, Ti, Th, Zr, Cu, Fe in the topsoil, while the subsoil was dominated by all the PTEs (factor 1 to 6) except Ba. The study concludes that indiscriminate human activities have an enormous effect on soil pollution.

• Klíčová slova
• Contamination degree, European average value, Potentially toxic elements, Soil pollution, Source apportionment, Spatial distribution,
• MeSH
• hodnocení rizik MeSH
• látky znečišťující půdu analýza   toxicita   MeSH
• lidé MeSH
• monitorování životního prostředí MeSH
• prostorová analýza MeSH
• půda chemie   MeSH
• těžké kovy analýza   toxicita   MeSH
• znečištění životního prostředí analýza   statistika a číselné údaje   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• látky znečišťující půdu MeSH
• půda MeSH
• těžké kovy MeSH

The bacterial endosymbiont Wolbachia has been used to control insect pests owing to its ability to manipulate their life history and suppress infectious diseases. Therefore, knowledge on Wolbachia dynamics in natural populations is fundamental. The European cherry fruit fly, Rhagoletis cerasi, is infected with the Wolbachia strain wCer2, mainly present in southern and central European populations, and is currently spreading into wCer2-uninfected populations driven by high unidirectional cytoplasmic incompatibility. Here, we describe the distribution of wCer2 along two transition zones where the infection is spreading into wCer2-uninfected R. cerasi populations. Fine-scale sampling of 19 populations in the Czech Republic showed a smooth decrease of wCer2 frequency from south to north within a distance of less than 20 km. Sampling of 12 Hungarian populations, however, showed a sharp decline of wCer2 infection frequency within a few kilometres. We fitted a standard wave equation to our empirical data and estimated a Wolbachia wave speed of 1.9 km yr-1 in the Czech Republic and 1.0 km yr-1 in Hungary. Considering the univoltine life cycle and limited dispersal ability of R. cerasi, our study highlights a rapid Wolbachia spread in natural host populations.

• Klíčová slova
• European cherry fruit fly, cytoplasmic incompatibility, endosymbiont, modelling,
• MeSH
• prostorová analýza * MeSH
• Tephritidae mikrobiologie   MeSH
• Wolbachia fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Maďarsko MeSH

BACKGROUND: Severe canopy-removing disturbances are native to many temperate forests and radically alter stand structure, but biotic legacies (surviving elements or patterns) can lend continuity to ecosystem function after such events. Poorly understood is the degree to which the structural complexity of an old-growth forest carries over to the next stand. We asked how pre-disturbance spatial pattern acts as a legacy to influence post-disturbance stand structure, and how this legacy influences the structural diversity within the early-seral stand. METHODS: Two stem-mapped one-hectare forest plots in the Czech Republic experienced a severe bark beetle outbreak, thus providing before-and-after data on spatial patterns in live and dead trees, crown projections, down logs, and herb cover. RESULTS: Post-disturbance stands were dominated by an advanced regeneration layer present before the disturbance. Both major species, Norway spruce (Picea abies) and rowan (Sorbus aucuparia), were strongly self-aggregated and also clustered to former canopy trees, pre-disturbance snags, stumps and logs, suggesting positive overstory to understory neighbourhood effects. Thus, although the disturbance dramatically reduced the stand's height profile with ~100% mortality of the canopy layer, the spatial structure of post-disturbance stands still closely reflected the pre-disturbance structure. The former upper tree layer influenced advanced regeneration through microsite and light limitation. Under formerly dense canopies, regeneration density was high but relatively homogeneous in height; while in former small gaps with greater herb cover, regeneration density was lower but with greater heterogeneity in heights. CONCLUSION: These findings suggest that pre-disturbance spatial patterns of forests can persist through severe canopy-removing disturbance, and determine the spatial structure of the succeeding stand. Such patterns constitute a subtle but key legacy effect, promoting structural complexity in early-seral forests as well as variable successional pathways and rates. This influence suggests a continuity in spatial ecosystem structure that may well persist through multiple forest generations.

• MeSH
• biodiverzita * MeSH
• ekosystém MeSH
• lesy * MeSH
• prostorová analýza * MeSH
• regenerace MeSH
• smrk anatomie a histologie   klasifikace   růst a vývoj   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Brucellosis in cattle and humans has received world-wide research attention as a neglected and re-emerging zoonotic disease with many routes of transmission. Studies of brucellosis in Uganda have emphasized occupational exposures and also revealed variations in prevalence levels by region and cattle production systems. To date, research linking pastoralist household income from dairy production to brucellosis and its transmission risk pathways do not exist in Uganda. We assessed whether spatial differences in unit milk prices can be explained by brucellosis prevalence in cattle along a distance gradient from Lake Mburo National Park in Uganda. Semi-structured interviews administered to 366 randomly selected household heads were supplemented with serological data on brucellosis in cattle. Statistical analysis included Pearson correlation test, multiple regression and analysis of variance (ANOVA) using SPSS version 17. Serological results showed that 44% of cattle blood samples were sero-positive for brucellosis. The results obtained from interviews put the statistical mean of household reported cattle abortions at 5.39 (5.08-5.70 at 95% CI, n=366). Post-hoc analysis of variance revealed that both sero-positive cattle and reported cattle abortions significantly were much lower when moving outwards from the park boundary (p<0.05), while the price of milk increased significantly (p<0.05) along the same distance gradient. Further studies should identify public and private partnerships needed to create and strengthen good zoonotic brucellosis management practices at the nexus of wildlife and livestock in Uganda.

• Klíčová slova
• Brucellosis, Dairy production, Household income, Spatial pattern, Uganda,
• MeSH
• brucelóza epidemiologie   veterinární   MeSH
• dobytek MeSH
• lidé MeSH
• mlékárenství * MeSH
• mléko ekonomika   MeSH
• nemoci skotu epidemiologie   MeSH
• prevalence MeSH
• prostorová analýza MeSH
• riziko MeSH
• skot MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Uganda epidemiologie   MeSH

Spatial transcriptomics is revolutionizing modern biology, offering researchers an unprecedented ability to unravel intricate gene expression patterns within tissues. From pioneering techniques to newly commercialized platforms, the field of spatial transcriptomics has evolved rapidly, ushering in a new era of understanding across various disciplines, from developmental biology to disease research. This dynamic expansion is reflected in the rapidly growing number of technologies and data analysis techniques developed and introduced. However, the expanding landscape presents a considerable challenge for researchers, especially newcomers to the field, as staying informed about these advancements becomes increasingly complex. To address this challenge, we have prepared an updated review with a particular focus on technologies that have reached commercialization and are, therefore, accessible to a broad spectrum of potential new users. In this review, we present the fundamental principles of spatial transcriptomic methods, discuss the challenges in data analysis, provide insights into experimental considerations, offer information about available resources for spatial transcriptomics, and conclude with a guide for method selection and a forward-looking perspective. Our aim is to serve as a guiding resource for both experienced users and newcomers navigating the complex realm of spatial transcriptomics in this era of rapid development. We intend to equip researchers with the necessary knowledge to make informed decisions and contribute to the cutting-edge research that spatial transcriptomics offers.

• Klíčová slova
• Commercial platforms, Selection guide, Spatial transcriptomics, Technologies,
• MeSH
• lidé MeSH
• stanovení celkové genové exprese * metody   MeSH
• transkriptom * MeSH
• výpočetní biologie metody   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

OBJECTIVES: To compare spatial inequalities in life expectancy (LE) in West Central Scotland (WCS) with nine other postindustrial European regions. DESIGN: A cross-sectional observational study. SETTING: WCS and nine other postindustrial regions across Europe. PARTICIPANTS: Data for WCS and nine other comparably deindustrialised European regions were analysed. Male and female LEs at birth were obtained or calculated for the mid-2000s for 160 districts within selected regions. Districts were stratified into two groups: small (populations of between 141 000 and 185 000 people) and large (populations between 224 000 and 352 000). The range and IQR in LE were used to describe within-region disparities. RESULTS: In small districts, the male LE range was widest in WCS and Merseyside, while the IQR was widest in WCS and Northern Ireland. For women, the LE range was widest in WCS, though the IQR was widest in Northern Ireland and Merseyside. In large districts, the range and IQR in LE was widest in WCS and Wallonia for both sexes. CONCLUSIONS: Subregional spatial inequalities in LE in WCS are wide compared with other postindustrial mainland European regions, especially for men. Future research could explore the contribution of economic, social and political factors in reducing these inequalities.

• Klíčová slova
• PUBLIC HEALTH, SOCIAL MEDICINE, STATISTICS & RESEARCH METHODS,
• MeSH
• lidé MeSH
• naděje dožití * MeSH
• prostorová analýza MeSH
• průřezové studie MeSH
• senioři MeSH
• socioekonomické faktory MeSH
• vyspělé země MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• pozorovací studie MeSH
• srovnávací studie MeSH
• Geografické názvy
• Evropa MeSH
• Skotsko MeSH