Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15048921
DOI
10.1002/jnr.20041
Knihovny.cz E-resources
- MeSH
- Bone Marrow Cells metabolism ultrastructure MeSH
- Microscopy, Electron methods MeSH
- Embryo, Mammalian MeSH
- Phosphopyruvate Hydratase metabolism MeSH
- Glial Fibrillary Acidic Protein metabolism MeSH
- Immunohistochemistry methods MeSH
- Stem Cells metabolism ultrastructure MeSH
- Spinal Cord Compression metabolism pathology therapy MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Luminescent Proteins metabolism MeSH
- Magnetic Resonance Imaging methods MeSH
- Spinal Cord cytology metabolism transplantation ultrastructure MeSH
- Brain cytology metabolism ultrastructure MeSH
- Mice MeSH
- Neuroglia metabolism pathology ultrastructure MeSH
- Neurons metabolism pathology ultrastructure MeSH
- Brain Injuries metabolism pathology therapy MeSH
- Rats, Wistar MeSH
- Stem Cell Transplantation methods MeSH
- Bone Marrow Transplantation methods MeSH
- Green Fluorescent Proteins MeSH
- Ferric Compounds metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- ferric oxide MeSH Browser
- Phosphopyruvate Hydratase MeSH
- Glial Fibrillary Acidic Protein MeSH
- Luminescent Proteins MeSH
- Green Fluorescent Proteins MeSH
- Ferric Compounds MeSH
Nuclear magnetic resonance (MR) imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied, in animals with a cortical photochemical lesion or with a balloon-induced spinal cord compression lesion, the fate of implanted rat bone marrow stromal cells (MSCs) and mouse embryonic stem cells (ESCs) labeled with superparamagnetic iron oxide nanoparticles (Endorem). MSCs were colabeled with bromodeoxyuridine (BrdU), and ESCs were transfected with pEGFP-C1 (eGFP ESCs). Cells were either grafted intracerebrally into the contralateral hemisphere of the adult rat brain or injected intravenously. In vivo MR imaging was used to track their fate; Prussian blue staining and electron microscopy confirmed the presence of iron oxide nanoparticles inside the cells. During the first week postimplantation, grafted cells migrated to the lesion site and populated the border zone of the lesion. Less than 3% of MSCs differentiated into neurons and none into astrocytes; 5% of eGFP ESCs differentiated into neurons, whereas 70% of eGFP ESCs became astrocytes. The implanted cells were visible on MR images as a hypointense area at the injection site, in the corpus callosum and in the lesion. The hypointense signal persisted for more than 50 days. The presence of GFP-positive or BrdU-positive and nanoparticle-labeled cells was confirmed by histological staining. Our study demonstrates that both grafted MSCs and eGFP ESCs labeled with a contrast agent based on iron oxide nanoparticles migrate into the injured CNS. Iron oxide nanoparticles can therefore be used as a marker for the long-term noninvasive MR tracking of implanted stem cells.
References provided by Crossref.org
Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis
The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages
Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
