Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- 1-Naphthyl phosphate, Alkaline phosphatase, Bacteria, Electrochemical detection, Magnetic particles, Planetary science, Remote sensing,
- MeSH
- Alkaline Phosphatase metabolism MeSH
- Equipment Design MeSH
- Electrochemical Techniques instrumentation MeSH
- Escherichia coli enzymology isolation & purification MeSH
- Limit of Detection MeSH
- Magnetite Nanoparticles chemistry MeSH
- Environmental Microbiology * MeSH
- Microfluidic Analytical Techniques instrumentation MeSH
- Naphthols isolation & purification MeSH
- Robotics instrumentation MeSH
- Staphylococcus aureus enzymology isolation & purification MeSH
- Remote Sensing Technology instrumentation MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-naphthol MeSH Browser
- Alkaline Phosphatase MeSH
- Magnetite Nanoparticles MeSH
- Naphthols MeSH
Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 μL. The flow rate was optimized to 500 μL/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticles modified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per μL.
References provided by Crossref.org
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