Oil-based adjuvants delivered intradermally induce high primary IgG2 immune response in swine
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
28319826
DOI
10.1016/j.rvsc.2017.03.007
PII: S0034-5288(17)30047-4
Knihovny.cz E-resources
- Keywords
- Adjuvant, Immunity, Intradermal, Th1/Th2, Vaccine,
- MeSH
- Adjuvants, Immunologic pharmacology MeSH
- Antigens administration & dosage MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Freund's Adjuvant pharmacology MeSH
- Hemocyanins pharmacology MeSH
- Immunization MeSH
- Immunoglobulin G blood MeSH
- Humans MeSH
- Lipids pharmacology MeSH
- Swine * MeSH
- Vaccination veterinary MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Antigens MeSH
- Freund's Adjuvant MeSH
- Hemocyanins MeSH
- Immunoglobulin G MeSH
- incomplete Freund's adjuvant MeSH Browser
- keyhole-limpet hemocyanin MeSH Browser
- Lipids MeSH
The effects of intradermal application of antigen with or without different adjuvants and activation of immune response are presented in this study. Six groups of six piglets each were immunized with keyhole limpet haemocyanin (KLH) antigen in combination with aluminium hydroxide or oil-based adjuvants (complete and incomplete Freund's adjuvants, Montanide ISA 206 and Emulsigen). IgG1 and IgG2 levels in sera were measured by KLH-specific ELISA. Interestingly, oil-based adjuvants induced high primary IgG2 response, suggesting the Th1 lymphocyte polarization. Also, considering the similarities between human and porcine organism, we suggest that intradermal application could be considered as an effective vaccine delivery route in both veterinary and human medicine.
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