Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29675784
DOI
10.1007/7651_2018_137
Knihovny.cz E-resources
- Keywords
- Cancer microenvironment, Cancer-associated fibroblast, Cell cultivation, Fibroblast, Fibroblast isolation,
- MeSH
- Biomedical Research * MeSH
- Cancer-Associated Fibroblasts pathology MeSH
- Fibroblasts cytology MeSH
- Cells, Cultured MeSH
- Skin cytology MeSH
- Humans MeSH
- Neoplasms pathology MeSH
- Cell Separation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cancer-associated fibroblasts (CAFs) represent a crucial component of cancer microenvironment. CAFs significantly influence biological properties of various types of cancer in terms of local aggressiveness, recurrence, and metastatic behaviour. This chapter summarizes a simple protocol for isolation of normal fibroblasts and their cancer-associated counterparts from normal human skin and mucosa, respectively, as well as from samples of human tumours such as basal/squamous carcinoma, melanoma, and breast cancer, and employment of this procedure for other types of cancer is possible. Isolated fibroblasts can be expanded in vitro and employed for further analysis of, e.g., DNA, RNA, protein, etc.
References provided by Crossref.org
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