Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step-by-step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana. Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae). © 2016 by John Wiley & Sons, Inc.

Central European Institute of Technology Masaryk University Brno Czech Republic

See more in PubMed

Armstrong, S.J. and Jones, G.H. 2001. Female meiosis in wild-type Arabidopsis thaliana and in two meiotic mutants. Sex. Plant Reprod. 13:177-183. doi: 10.1007/s004970000050.

Mandáková, T. and Lysak, M.A. 2016. Painting of Arabidopsis chromosomes with chromosome-specific BAC clones. Curr. Protoc. Plant Biol. In press.

Ross, K.J. , Fransz, P. , and Jones, G.H. 1996. A light microscopic atlas of meiosis in Arabidopsis thaliana . Chrom. Res. 4:507-516. doi: 10.1007/BF02261778

Diploid and tetraploid cytotypes of the flagship Cape species Dicerothamnus rhinocerotis (Asteraceae): variation in distribution, ecological niche, morphology and genetics

Ancient hybridization and repetitive element proliferation in the evolutionary history of the monocot genus Amomum (Zingiberaceae)

A chromosome-level genome assembly for the amphibious plant Rorippa aquatica reveals its allotetraploid origin and mechanisms of heterophylly upon submergence

Pervasive Introgression During Rapid Diversification of the European Mountain Genus Soldanella (L.) (Primulaceae)

The evolution of the hypotetraploid Catolobus pendulus genome - the poorly known sister species of Capsella

Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes

The relationship between transposable elements and ecological niches in the Greater Cape Floristic Region: A study on the genus Pteronia (Asteraceae)

The evolutionary history of Cardamine bulbifera shows a successful rapid postglacial Eurasian range expansion in the absence of sexual reproduction

Genome diploidization associates with cladogenesis, trait disparity, and plastid gene evolution

Evolution of an Apomixis-Specific Allele Class in Supernumerary Chromatin of Apomictic Boechera

The Sister Chromatid Division of the Heteromorphic Sex Chromosomes in Silene Species and Their Transmissibility towards the Mitosis

Integrative Study of Genotypic and Phenotypic Diversity in the Eurasian Orchid Genus Neotinea

Gradual evolution of allopolyploidy in Arabidopsis suecica

Linked by Ancestral Bonds: Multiple Whole-Genome Duplications and Reticulate Evolution in a Brassicaceae Tribe

Allele Sorting as a Novel Approach to Resolving the Origin of Allotetraploids Using Hyb-Seq Data: A Case Study of the Balkan Mountain Endemic Cardamine barbaraeoides

The genome of Draba nivalis shows signatures of adaptation to the extreme environmental stresses of the Arctic

Chromosomal Evolution and Apomixis in the Cruciferous Tribe Boechereae

Genome Evolution in Arabideae Was Marked by Frequent Centromere Repositioning

Evolution of Tandem Repeats Is Mirroring Post-polyploid Cladogenesis in Heliophila (Brassicaceae)

Origin and Evolution of Diploid and Allopolyploid Camelina Genomes Were Accompanied by Chromosome Shattering