Kazuistika pojednává o pacientce s dlouhodobě nedostatečně kompenzovaným diabetem 2. typu, u které bylo dosaženo úspěchu v léčbě nasazením nové fixní kombinace bazálního inzulínu a GLP-1 agonisty.
A case describes a patient with a prolonged inadequate compensation of type 2 diabetes, in whom has success new therapy with fixed combination of insulin degludec and liraglutide.
- MeSH
- diabetes mellitus 2. typu * farmakoterapie MeSH
- dlouhodobě působící inzulin aplikace a dávkování metabolismus MeSH
- fixní kombinace léků MeSH
- glykovaný hemoglobin účinky léků MeSH
- hmotnostní úbytek účinky léků MeSH
- lidé středního věku MeSH
- lidé MeSH
- liraglutid aplikace a dávkování metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Cancer is a serious disease that causes 25% of deaths in the developed countries. Significant impact on the cancer patients survival has early detection of this disease, therefore great attention is paid to its imaging. Fluorescence imaging represents powerful imaging method for the cell detection. For the successful detection of tumour cells, the specific targeting of fluorescence probes to the tumour tissue has a key role. Interesting materials enabling the imaging of tumour cells are fluorescence nanoparticles. For the accurate imaging, the NPs should be conjugated with targeting ligands and/or constructed as off-on probes.
Doxorubicin (DOX) is an effective antitumor drug employed for treatment of a wide range of cancers types such as neuroblastoma, osteosarcoma, breast and esophageal carcinomas. On the other hand, the cumulative dose is restricted (300-550 mg/m(2)) and its amount administered to a patient has to be closely controlled due to its cardiotoxicity. To understand the mechanisms of the DOX side effects as well as to reveal the ways how to reduce its adverse impact on cardiomyocytes, the interactions with particular components of the blood and tissues have to be studied in greater detail. In this work, microdialysis technique was optimized to extract DOX from samples and subsequently monitor its interaction with BSA. Finally, the microdialysis probe was connected on-line to the LIF detector to ensure the real-time detection. The best flow rate was 1 μL/min and after 120 min of microdialysis 28% of the DOX was dialyzed out from the sample. The results from investigation of the DOX-BSA interaction indicate that the interaction occurs in less than 30 min, causing marked decrease in the amount of DOX extracted by microdialysis.
- MeSH
- doxorubicin chemie MeSH
- fluorescence MeSH
- lidé MeSH
- limita detekce MeSH
- mikrodialýza MeSH
- sérový albumin hovězí chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Quantum dots (QDs) are small semiconductor nanoparticles with great optical properties. Their behaviour enables the usage of QDs in in vitro and in vivo experiments and they are promising tools in disease treatment and targeted therapy. The limitation of their usage is the toxicity. Quantum dots consist of different metals, which have various effects on the health. To decrease their toxicity, different surface coatings are used. The effect of QDs on the organism can be tested on chicken embryos. Chicken embryos represent great model for QDs toxicity studies, because there is no need of any permission for the work with embryos and the experiments are low cost and fast.
This review is aimed on the interaction of quantum dots with tumor cells by fluorescence microscopy, we analyze the possibilities of the usage of QDs in tumor cell detection and visualization by fluorescence microscopy. QDs are fluorescent nanoparticles with good fluorescence properties. Specifically modified QDs can be targeted to the tumor tissue. QDs can be modified by antibodies, proteins, carbohydrates etc. For the use of QDs in biological applications, their solubility in water, stability and biocompatibility is important. Fluorescence microscopy is a good tool to observe interaction of QDs with cells and enable direct differentiation of normal and tumor cells.
- MeSH
- fluorescence MeSH
- fluorescenční mikroskopie * MeSH
- kvantové tečky * MeSH
- lidé MeSH
- nádory * diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Carbon nanomaterials, including fullerenes, exhibit not only unique structure and electronic properties but also a significant potential to serve as radical scavengers and/or anti-oxidants. Their conjugation with anticancer drugs such as doxorubicin (DOX) may help to balance severe negative side effects of these cytostatics and also improve the delivery of the drug taking advantage of the enhanced cellular uptake, selectivity to cancer cells, and pH regulated release. In this study, the fullerene (C60) surface was oxidized by concentrated nitric acid, which enabled simple DOX-fullerene conjugation based on π-π stacking and hydrophilic interactions with carboxylic groups. The strength of this noncovalent binding is pH dependent. At a low pH, the amino group of DOX is protonated, however at a higher pH, the amino group is deprotonated, resulting in stronger hydrophobic interactions with the fullerene walls. CE and HPLC were employed for characterization of resulting complexes. The cell toxicity of the conjugates was evaluated using Staphylococcus aureus and finally they were administered into the chicken embryo to assess the applicability for in vivo imaging.
- MeSH
- antibakteriální látky chemie farmakokinetika farmakologie MeSH
- doxorubicin chemie farmakokinetika farmakologie MeSH
- fluorescenční spektrometrie MeSH
- fullereny chemie farmakokinetika MeSH
- hydrofobní a hydrofilní interakce MeSH
- kuřecí embryo MeSH
- nosiče léků chemie farmakokinetika MeSH
- Staphylococcus aureus účinky léků MeSH
- tkáňová distribuce MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Magnetic particle mediated transport in combination with nanomaterial based drug carrier has a great potential for targeted cancer therapy. In this study, doxorubicin encapsulation into the apoferritin and its conjugation with magnetic particles was investigated by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The quantification of encapsulated doxorubicin was performed by fluorescence spectroscopy and compared to CE-LIF. Moreover, the significant enhancement of the doxorubicin signal was observed by addition of methanol into the sample solution.
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
