Apoptosis during tooth development appears dependent on the apoptotic executioner caspase-3, but not caspase-7. Instead, activated caspase-7 has been found in differentiated odontoblasts and ameloblasts, where it does not correlate with apoptosis. To further investigate these findings, the mouse incisor was used as a model. Analysis of caspase-7-deficient mice revealed a significant thinner layer of hard tissue in the adult incisor. Micro computed tomography scan confirmed this decrease in mineralized tissues. These data strongly suggest that caspase-7 might be directly involved in functional cell differentiation and regulation of the mineralization of dental matrices.
- MeSH
- ameloblasty cytologie enzymologie metabolismus MeSH
- buněčná diferenciace * MeSH
- časové faktory MeSH
- imunohistochemie MeSH
- kaspasa 7 genetika metabolismus MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- odontoblasty cytologie enzymologie metabolismus MeSH
- odontogeneze MeSH
- proliferace buněk MeSH
- rentgenová mikrotomografie MeSH
- řezáky embryologie růst a vývoj metabolismus MeSH
- zubní sklovina embryologie růst a vývoj metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Apoptosis is an important morphogenetic event in embryogenesis as well as during postnatal life. In the last 2 decades, apoptosis in tooth development (odontogenesis) has been investigated with gradually increasing focus on the mechanisms and signaling pathways involved. The molecular machinery responsible for apoptosis exhibits a high degree of conservation but also organ and tissue specific patterns. This review aims to discuss recent knowledge about apoptotic signaling networks during odontogenesis, concentrating on the mouse, which is often used as a model organism for human dentistry. Apoptosis accompanies the entire development of the tooth and corresponding remodeling of the surrounding bony tissue. It is most evident in its role in the elimination of signaling centers within developing teeth, removal of vestigal tooth germs, and in odontoblast and ameloblast organization during tooth mineralization. Dental apoptosis is caspase dependent and proceeds via mitochondrial mediated cell death with possible amplification by Fas-FasL signaling modulated by Bcl-2 family members.
- MeSH
- apoptóza MeSH
- kaspasy genetika metabolismus MeSH
- lidé MeSH
- myši MeSH
- odontogeneze MeSH
- signální transdukce MeSH
- zubní zárodek cytologie embryologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
The transcription factor c-Myb is involved in the control of cell proliferation, survival and differentiation. As these processes accompany the morphogenesis of developing teeth, this work investigates the possible role of c-Myb during odontogenesis. Analysis of the expression of c-Myb in the monophyodont mouse was followed by similar analysis in a diphyodont species, the pig, which has a dentition more closely resembling that of the human. The distribution of c-Myb was correlated with the pattern of proliferation and apoptosis and the tooth phenotype of c-Myb mutant mice was also assessed. In the mouse, c-Myb expression was detected throughout prenatal development of the first molar tooth. Negative temporospatial correlation was found between c-Myb expression and apoptosis, while c-Myb expression positively correlated with proliferation. c-Myb-positive cells, however, were more abundant than the proliferating cell nuclear antigen positive cells, suggesting other roles of c-Myb in odontogenesis. In the minipig, in contrast to the mouse, there was an asymmetrical arrangement of c-Myb positive cells, with a higher presence on the labial side of the tooth germ and dental lamina. A cluster of negative cells was situated in the mesenchyme close to the tooth bud. At later stages, the number of positive cells decreased and these cells were situated in the upper part of the dental papilla in the areas of future cusp formation. The expression of c-Myb in both species was strong in the odontoblasts and ameloblasts at the stage of dentin and enamel production suggesting a possible novel role of c-Myb during tooth mineralization.
- MeSH
- alely MeSH
- ameloblasty cytologie metabolismus MeSH
- apoptóza MeSH
- dentice MeSH
- druhová specificita MeSH
- embryo savčí cytologie embryologie metabolismus MeSH
- imunohistochemie MeSH
- klonování DNA MeSH
- koncové značení zlomů DNA in situ MeSH
- miniaturní prasata MeSH
- myši MeSH
- odontoblasty cytologie metabolismus MeSH
- odontogeneze MeSH
- osteoklasty cytologie metabolismus MeSH
- prasata MeSH
- proliferace buněk MeSH
- proliferační antigen buněčného jádra metabolismus MeSH
- proteiny buněčného cyklu genetika metabolismus MeSH
- protoonkogenní proteiny c-myb genetika metabolismus MeSH
- trans-aktivátory genetika metabolismus MeSH
- vývojová regulace genové exprese MeSH
- zuby cytologie embryologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
1. vyd. 61 s. : il. ; 21 cm
- MeSH
- biomedicínské technologie MeSH
- buněčný cyklus MeSH
- embryonální vývoj MeSH
- fyziologie MeSH
- lidé MeSH
- Nobelova cena MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Konspekt
- Obecná biologie
- NLK Obory
- fyziologie
- biomedicínské inženýrství
- NLK Publikační typ
- učebnice vysokých škol
Komplex vyvíjejícího se zubu a okolních struktur zahrnuje řadu typů diferencovaných buněk. Ty se často vyskytujíve velmi malých počtech, ale mají velký biologický význam v morfogenezi (např. signální centra sklovinných uzlů). Možnost izolace homogenních vzorků z konkrétních tkáňově vázaných buněčných populací byla dlouhou dobu omezena. Nová technologie systému laserové záchytové mikrodisekce (LCM) zprostředkovává řadu přesných výzkumů na buněčné a subbuněčné úrovni. V posledním desetiletí bylo techniky LCM využito také pro některé studie zaměřené na mechanismy vývoje zubů a související poruchy. Cílem tohoto článkuje stručné shrnutí přístupu LCM, jeho aplikací ve výzkumu odontogeneze i nejaktuálnějších modifikací kombinujících LCM a průtokovou cytometrii pro analýzy jednotlivých buněk na úrovni nukleových kyselin a proteinů.
Complex of developing tooth and surrounding structures comprises several types of differentiated cells. They often occur in very small numbers but with a high biological impact on morphogenesis (e. g. signalling centres of enamel knots). However, isolation of specific homogenous samples from distinct tissue bound cell populations was long time limited. With appearance of novel technologies, the laser capture microdissection (LCM) system mediates many exact investigations at the cellular and subcellular level. In the last decade, the LCM technique has been used also for several studies focused on mechanisms of tooth development and related disorders. This paper aims to briefly review the LCM approach, its application in odontogenesis research, and the most recent modification combining LCM and flow cytometry for single cell analysis at nucleid acid and protein levels.
