Hybrid biocomposite scaffolds (HBS) that serve as a carrier for cell proliferation and differentiation are increasingly used for tissue regeneration. 3D hybrid scaffold based on collagen-grafted-chitosan-glucan fiber (CO-g-CGF-HBS) was prepared by freeze-drying technique. The swelling percentage, hydrolytic stability, and modulus of elasticity of HBS were enhanced after the chemical modification of CO with CGF. Pore size and porosity of HBS were decreased with an increased CGF ratio. HBS exhibits a higher reduction rate against different types of bacteria compared with a control sample. Thus, chemical modification of CO with different ratios of CGF significantly improved the physicochemical, antibacterial properties of HBS.
This study presents an innovative multifunctional system in fabricating new functional wound dressing (FWD) products that could be used for skin regeneration, especially in cases of infected chronic wounds and ulcers. The innovation is based on the extraction, characterization, and application of collagen (CO)/chitosan-glucan complex hollow fibers (CSGC)/aloe vera (AV) as a novel FWS. For the first time, specific hollow fibers were extracted with controlled inner (500-900 nm)/outer (2-3 µm) diameters from mycelium of Schizophyllum commune. Further on, research and evaluation of morphology, hydrolytic stability, and swelling characteristics of CO/CSGC@AV were carried out. The obtained FWS showed high hydrolytic stability with enhanced swelling characteristics compared to native collagen. The hemostatic effect of FWS increased significantly in the presence of CSGC, compared to native CO and displayed excellent biocompatibility which was tested by using normal human dermal fibroblast (NHDF). The FWS showed high antibacterial activity against different types of bacteria (positive/negative grams). From in vivo measurements, the novel FWS increased the percentage of wound closure after one week of treatment. All these results imply that the new CO/CSGC@AV-FWD has the potential for clinical skin regeneration and applying for controlled drug release.
- MeSH
- Aloe * chemie MeSH
- antibakteriální látky chemie izolace a purifikace farmakologie MeSH
- Bacteria účinky léků růst a vývoj MeSH
- chitosan chemie izolace a purifikace farmakologie MeSH
- fibrilární kolageny chemie izolace a purifikace farmakologie MeSH
- glukany chemie izolace a purifikace farmakologie MeSH
- hojení ran účinky léků MeSH
- kultivované buňky MeSH
- kůže účinky léků zranění patologie MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- mycelium * chemie MeSH
- obvazy * MeSH
- potkani Wistar MeSH
- rány a poranění farmakoterapie patologie MeSH
- rostlinné extrakty chemie izolace a purifikace farmakologie MeSH
- Schizophyllum * chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Novel nanofibers mats were fabricated by electrospinning of polyvinyl alcohol/hyaluronan (PVA/HA) solutions in the presence of silver nanoparticles (AgNPs). The AgNPs were synthesized by in-situ chemical reduction of silver ions (Ag+) using HA as a reducing and stabilizing agent. Narrow size distribution and spherical shape of AgNPs were achieved by optimizing the initial silver nitrate concentration (0.01 to 1 M) and reaction time (10-60 min). HA-AgNPs nanocomposite and PVA/HA-AgNPs nanofibrous mats were fabricated by electrospinning technique from aqueous solution containing a different mass ratio of PVA and HA-AgNPs and characterized by UV/Vis spectroscopy, SEM, TEM, DLS, XRD, TGA, and ATR-FTIR. Mechanical and rheological properties were also investigated and discussed. The novel nanofibrous mats show great potential in skin regeneration and drug carrier applications.
- MeSH
- dusičnan stříbrný chemie MeSH
- elektrochemie MeSH
- ionty MeSH
- kovové nanočástice chemie MeSH
- kyselina hyaluronová chemie MeSH
- nanokompozity chemie MeSH
- nanovlákna MeSH
- nosiče léků MeSH
- obvazy MeSH
- polyvinylalkohol chemie MeSH
- povrchové vlastnosti MeSH
- reologie MeSH
- rozpouštědla MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- stříbro chemie MeSH
- technologie zelené chemie * MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
- MeSH
- hojení ran účinky léků MeSH
- krysa rodu rattus MeSH
- krystalizace MeSH
- kultivované buňky MeSH
- kyselina hyaluronová chemie terapeutické užití MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- nanovlákna chemie terapeutické užití ultrastruktura MeSH
- obvazy * MeSH
- stabilita léku MeSH
- stříbro chemie MeSH
- velikost částic MeSH
- vřed farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Silver/Alginate/Nicotinamide nanoparticles composite (Ag/ALG/Nic) was prepared and used for the first time to fabricate wound dressing material. Sodium alginate (ALG) was used as reducing and stabilizing agents for preparation of silver nanoparticles (Ag-NPs). Effect of concentrations of alginate (ALG) on the particle size of silver were studied and confirmed by different techniques like UV/vis spectroscopy, transmission electron microscope (TEM) and dynamic light scattering (DLS). Nonwoven viscous fabrics were used as a carrier for silver/alginate/nanoparticles composite by impregnated the nonwoven fabrics as per the padding-curing technique. Nicotinamide (Nic) as anti-inflammatory drug was entrapped into Ag-NPS/ALG/nonwoven fabrics. Scanning electron microscope and energy dispersive x-ray (SEM-EDX) were used to evaluate the presence of Ag/ALG/Nic nanoparticles composite anchored the nonwoven fabrics. The antibacterial activity of the Ag/ALG/Nic wound dressing material was evaluated against Escherichia coli (E. coli) and Staphylococcus Aureus (St. Aureus). The wound healing and histological studied were evaluated by using burn diabetic rat animals.
- MeSH
- algináty chemie MeSH
- antiflogistika nesteroidní farmakologie MeSH
- aplikace kožní MeSH
- Escherichia coli účinky léků růst a vývoj MeSH
- experimentální diabetes mellitus chemicky indukované komplikace farmakoterapie mikrobiologie MeSH
- infekce vyvolané Escherichia coli komplikace farmakoterapie mikrobiologie patologie MeSH
- kovové nanočástice chemie ultrastruktura MeSH
- králíci MeSH
- krysa rodu rattus MeSH
- kůže účinky léků mikrobiologie patologie MeSH
- kyselina glukuronová chemie MeSH
- kyseliny hexuronové chemie MeSH
- nanokompozity chemie ultrastruktura MeSH
- niacinamid farmakologie MeSH
- obvazy * MeSH
- popálení farmakoterapie mikrobiologie patologie MeSH
- stafylokokové infekce komplikace farmakoterapie mikrobiologie patologie MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- streptozocin MeSH
- stříbro chemie MeSH
- textilie MeSH
- velikost částic MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Chitin/chitosan-glucan complex (ChCsGC) was isolated from Schizophyllum commune (S. commune) and dissolved for the first time in precooled (-15°C) 8wt.% urea/6wt.% NaOH aqueous solution. Novel nonwoven microfiber mats were fabricated by wet-dry-spinning technique and evaluated the mechanical of fabrics mats and surface morphology. Isolated and nonwoven mat were characterized employing FTIR-ATR, Optical microscope, TGA, DSC, H/C NMR, SEM and XRD techniques. According to the physical/chemical characterization measurements we can assumed that, the net and the novel dressing mats have the same chemical structure with slightly changes in the thermal stability for the dressing mats.The biological activity of the nonwoven ChCsGC fabric was tested against different types of bacteria exhibiting excellent antibacterial activity. Cell viability of the plain complex and nonwovens mats were evaluated utilizing mouse fibroblast cell line varying concentrations and treatment time. ChCsGC did not show any cytotoxicity against mouse fibroblast cells and the cell-fabrics interaction was also investigated using fluorescence microscope. The novel ChCsGC nonwovens exhibited excellent surgical wound healing ability when tested using rat models.
- MeSH
- antibakteriální látky aplikace a dávkování chemie MeSH
- buňky NIH 3T3 MeSH
- chitosan aplikace a dávkování chemie izolace a purifikace MeSH
- glukany aplikace a dávkování chemie izolace a purifikace MeSH
- hojení ran účinky léků MeSH
- krysa rodu rattus MeSH
- myši MeSH
- obvazy * MeSH
- proliferace buněk účinky léků MeSH
- Schizophyllum izolace a purifikace MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Recruitment of cells and mediators is altered during impaired wound healing, thereby delaying this process. To overcome this problem, the correlation of wound healing in older rats, and the impact of different molecular weight of hyaluronan without silver nanoparticles; (low-HA1), (High-HA2), (Medium- HA3) and with silver nanoparticles (High-HA4) is investigated. The superior HA were selected to be further investigated onto diabetic wounds. Our results pointed to a marked deficiency in wounds granulation in older rats, which was accompanied with impairment of healing process. In older rats group treated with HA2 or HA4, granulation and dermal construction were improved. Furthermore, the number of pathogenic bacteria on wounds was declined throughout the first 24h by HA2 and HA4. The wound size in HA4-treated older rats was significantly smaller than that in other HA1, HA2 or HA3-treated older ones. Also, diabetes impaired the level of inflammatory cytokine, in diabetic model. On contrary, HA4 was found to normalize the level of inflammatory cytokine, in the diabetic model. Furthermore, HA4 was found to recover all oxidative and toxicity markers in diabetic models. This data confirms the critical role of HA4 to improve granulation and inflammatory mediators in impaired older and diabetic rat wound healing.
- MeSH
- experimentální diabetes mellitus komplikace farmakoterapie mikrobiologie patologie MeSH
- hojení ran účinky léků MeSH
- kovové nanočástice aplikace a dávkování chemie MeSH
- krysa rodu rattus MeSH
- kyselina hyaluronová aplikace a dávkování chemie MeSH
- lidé MeSH
- molekulová hmotnost MeSH
- stříbro aplikace a dávkování chemie MeSH
- zánět komplikace farmakoterapie mikrobiologie patologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
In this work we have used X-ray micro-computed tomography (μCT) as a method to observe the morphology of 3D porous pure collagen and collagen-composite scaffolds useful in tissue engineering. Two aspects of visualizations were taken into consideration: improvement of the scan and investigation of its sensitivity to the scan parameters. Due to the low material density some parts of collagen scaffolds are invisible in a μCT scan. Therefore, here we present different contrast agents, which increase the contrast of the scanned biopolymeric sample for μCT visualization. The increase of contrast of collagenous scaffolds was performed with ceramic hydroxyapatite microparticles (HAp), silver ions (Ag(+)) and silver nanoparticles (Ag-NPs). Since a relatively small change in imaging parameters (e.g. in 3D volume rendering, threshold value and μCT acquisition conditions) leads to a completely different visualized pattern, we have optimized these parameters to obtain the most realistic picture for visual and qualitative evaluation of the biopolymeric scaffold. Moreover, scaffold images were stereoscopically visualized in order to better see the 3D biopolymer composite scaffold morphology. However, the optimized visualization has some discontinuities in zoomed view, which can be problematic for further analysis of interconnected pores by commonly used numerical methods. Therefore, we applied the locally adaptive method to solve discontinuities issue. The combination of contrast agent and imaging techniques presented in this paper help us to better understand the structure and morphology of the biopolymeric scaffold that is crucial in the design of new biomaterials useful in tissue engineering.
- MeSH
- biokompatibilní materiály chemie MeSH
- hydroxyapatit chemie MeSH
- kolagen chemie MeSH
- kontrastní látky MeSH
- kovové nanočástice chemie MeSH
- rentgenová mikrotomografie * MeSH
- stříbro chemie MeSH
- tkáňové podpůrné struktury chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)/nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
- MeSH
- antibakteriální látky chemie terapeutické užití MeSH
- buňky NIH 3T3 MeSH
- chitosan chemie terapeutické užití MeSH
- Escherichia coli účinky léků patogenita MeSH
- hojení ran účinky léků MeSH
- kyselina hyaluronová chemie terapeutické užití MeSH
- lidé MeSH
- myši MeSH
- obvazy mikrobiologie MeSH
- Staphylococcus aureus účinky léků patogenita MeSH
- textilie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Chitin and chitosan were obtained by chemical treatments of shrimp shells. Different particle sizes (50-1000 μm) of the raw material were used to study their effect on size distribution, demineralization, deproteinization and deacetylation of chitin and chitosan isolation process. The particle size in the range of 800-1000 μm was selected to isolate chitin, which was achieved by measuring nitrogen, protein, ash, and yield %. Hydrochloric acid (5%, v/v) was optimized in demineralization step to remove the minerals from the starting material. Aqueous solution of sodium hydroxide (5%, w/v) at 90 °C for (20 h) was used in deproteinization step to remove the protein. Pure chitin was consequently impregnated into high concentration of sodium hydroxide (50%) for 3.5 h at 90 °C to remove the acetyl groups in order to form high pure chitosan. The degree of deacetylation (DDA) of chitosan was controlled and evaluated by different analytical tools. The chemical structure of chitin and chitosan was confirmed by elemental analysis, ATR-FTIR, H/C NMR, XRD, SEM, UV-Vis spectroscopy, TGA, and acid-base titration. The isolated chitin and chitosan from shrimp shell showed excellent antibacterial activity against Gram (-ve) bacteria (Escherichia coli) comparing with commercial biopolymers.
- MeSH
- antibakteriální látky izolace a purifikace farmakologie MeSH
- chitin izolace a purifikace farmakologie ultrastruktura MeSH
- chitosan izolace a purifikace farmakologie MeSH
- Decapoda (Crustacea) chemie MeSH
- difrakce rentgenového záření MeSH
- Escherichia coli účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- skořápky zvířat chemie MeSH
- stabilita léku MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Atlantský oceán MeSH
- Brazílie MeSH
