Zhodnotili jsme soubor pacientů po TEP kyčelního kloubu se zaměřením se na urologické komplikace, kterých je 16 %. U mužů je incidence komplikací celkem 21,8 %, 11 mužů ze 124 podstoupilo po implantaci TEP jeden nebo více urologických výkonů. U žen je 11,5 % urologických komplikací. Dvě ženy ze 157 podstoupily další urologické výkony. Všichni pacienti měli po operaci zavedený permanentní močový katétr. Průměrná doba jeho zavedení je 5 dní, u pacientů s komplikacemi 6 dní. Delší doba zavedení katétru zvyšuje riziko výskytu urologických komplikací. Rizikový pacient je muž, starší 65 let s pozitivní urologickou anamnézou a katétrem zavedeným 6 dní a více. Doporučujeme katétr zavádět jen u pacientů, kde to vyžaduje celkový stav a minimalizovat dobu jeho zavedení. Vhodná je volba správného katétru a jeho šetrné zavedení. V případě urologických komplikací včas kontaktovat urologa.

We have evaluated our group of patients after total hip arthroplasty focusing on the urologic complications of which there is 16 %. The incidence in men is 21.8 %. The total of 11 out of 124 men have undergone one or more urologic operations after the replacement. The incidence in women is 11.5 %. Meanwhile 2 out of 157 women have undergone other urologic procedures. All patients had indwelling catheter after the orthopaedic operation. The average indwelling period is 5 days in general, in patients with complications it is 6 days. Longer indwelling period increases the risk of urologic complication´s incidence. The risk factor is man over 65 years of age with positive urologic history and the catheter indwelling period of 6 days and more. We recommend to use catheter only when it is necessary for patient´s health and also we recommend to minimize the indwelling period. When performing indwelling, make sure you choose appropriate catheter and perform the indwelling gently. Also contact the urologist early if the urologic complications occur.

• MeSH
• časové faktory MeSH
• dospělí MeSH
• incidence MeSH
• infekce močového ústrojí epidemiologie   klasifikace   komplikace   MeSH
• katetrizace močového měchýře škodlivé účinky   MeSH
• katétrové infekce epidemiologie   komplikace   MeSH
• lidé středního věku MeSH
• lidé MeSH
• náhrada kyčelního kloubu MeSH
• pooperační komplikace epidemiologie   etiologie   MeSH
• retrospektivní studie MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• urologické nemoci epidemiologie   etiologie   komplikace   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH

Synovial membrane and synovial fluid represent a good source of mesenchymal stem cells. They have been regarded as a promising therapeutic tool for musculoskeletal regeneration. Synovium-derived mesenchymal stem cells have higher expression of CD44 and better chondrogenic potential in vitro than mesenchymal stem cells from other tissues. In this study we compared mesenchymal stem cells from synovium and synovial fluid on the base of morphological, immunophenotype and differentiation features. A heterogeneous population of cells with different morphology was obtained after isolation and 4-day cultivation. The mesenchymal stem cell immunophenotype was confirmed by positive expression of CD105, CD90, and CD44 by flow cytometry and cells were negative for CD45. CD105+ cells were selected by immunomagnetic separation after 2–4 weeks of cultivation. The percentage of CD105+ cells in the mesenchymal stem cell population from synovia was between 40–50 % before immunomagnetic separation and increased to 95 % following the immunomagnetic separation. Von Kossa, Alcian blue and Oil Red O staining was used to assess the differentiation potential of synovial mesenchymal stem cells. Long-term cultivation did not affect the morphology and immunophenotype of synovial mesenchymal stem cells. Our results confirmed that immunomagnetic separation based on CD 105 antigen is a suitable method to enrich the subpopulation of CD105+ synovial mesenchymal stem cells.

• MeSH
• buněčná diferenciace MeSH
• buněčný rodokmen MeSH
• CD antigeny metabolismus   MeSH
• chondrocyty cytologie   MeSH
• chondrogeneze MeSH
• imunofenotypizace MeSH
• imunomagnetická separace metody   MeSH
• lidé MeSH
• mezenchymální kmenové buňky cytologie   metabolismus   MeSH
• průtoková cytometrie MeSH
• receptory buněčného povrchu metabolismus   MeSH
• separace buněk metody   MeSH
• synoviální membrána cytologie   MeSH
• synoviální tekutina cytologie   MeSH
• Check Tag
• lidé MeSH

Telomeres are specialized chromatin structures that are situated at the end of linear chromosomes and play an important role in cell senescence and immortalization. Here, we investigated whether changes in histone signature influence the nuclear arrangement and positioning of telomeres. Analysis of mouse embryonic fibroblasts revealed that telomeres were organized into specific clusters that partially associated with centromeric clusters. This nuclear arrangement was influenced by deficiency of the histone methyltransferase SUV39h, LMNA deficiency, and the histone deacetylase inhibitor Trichostatin A (TSA). Similarly, nuclear radial distributions of telomeric clusters were preferentially influenced by TSA, which caused relocation of telomeres closer to the nuclear center. Telomeres also co-localized with promyelocytic leukemia bodies (PML). This association was increased by SUV39h deficiency and decreased by LMNA deficiency. These differences could be explained by differing levels of the telomerase subunit, TERT, in SUV39h- and LMNA-deficient fibroblasts. Taken together, our data show that SUV39h and A-type lamins likely play a key role in telomere maintenance and telomere nuclear architecture.

• MeSH
• DNA vazebné proteiny metabolismus   MeSH
• epigeneze genetická MeSH
• fibroblasty metabolismus   MeSH
• genová přestavba MeSH
• intranukleární inkluzní tělíska metabolismus   MeSH
• lamin typ A metabolismus   MeSH
• lidé MeSH
• methyltransferasy metabolismus   MeSH
• myši MeSH
• protein TRF1 metabolismus   MeSH
• průtoková cytometrie MeSH
• rap1 proteiny vázající GTP metabolismus   MeSH
• represorové proteiny metabolismus   MeSH
• telomerasa metabolismus   MeSH
• telomery genetika   metabolismus   MeSH
• transport proteinů MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and endonuclease G (endoG). Another interesting protein, which was expected to act similarly as AIF due to the high sequence homology with AIF is AIF-homologous mitochondrion-associated inducer of death (AMID). We studied the structure, cellular localization, and interactions of several proteins in silico and also in cells using fluorescent microscopy. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the lipid membranes. Bioinformatic predictions were conducted to analyze the interactions of the studied proteins with each other and with other possible partners. We conducted molecular modeling of proteins with unknown 3D structures. These models were then refined by MolProbity server and employed in molecular docking simulations of interactions. Our results show data acquired using a combination of modern in silico methods and image analysis to understand the localization, interactions and functions of proteins AMID, AIF, endonuclease G, and other apoptosis-related proteins.

• MeSH
• apoptóza MeSH
• biologické modely MeSH
• endonukleasy MeSH
• fluorescenční mikroskopie metody   MeSH
• kaspasy metabolismus   MeSH
• konformace proteinů MeSH
• lidé MeSH
• molekulární modely MeSH
• nádorové buněčné linie MeSH
• počítačová simulace MeSH
• proteomika metody   MeSH
• software MeSH
• terciární struktura proteinů MeSH
• vazba proteinů MeSH
• výpočetní biologie metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF.

• MeSH
• apoptóza fyziologie   MeSH
• buněčné jádro metabolismus   MeSH
• buněčné linie MeSH
• endodeoxyribonukleasy izolace a purifikace   metabolismus   MeSH
• faktor vyvolávající apoptózu izolace a purifikace   metabolismus   MeSH
• financování organizované MeSH
• fluorescenční mikroskopie MeSH
• Golgiho aparát metabolismus   MeSH
• intracelulární membrány metabolismus   MeSH
• lidé MeSH
• lyzozomy metabolismus   MeSH
• mitochondriální proteiny izolace a purifikace   metabolismus   MeSH
• mitochondrie metabolismus   MeSH
• proteiny regulující apoptózu izolace a purifikace   metabolismus   MeSH
• rekombinantní fúzní proteiny metabolismus   MeSH
• výpočetní biologie MeSH
• Check Tag
• lidé MeSH

An examination of the cellular response to DNA damage and the induction of p53 in human tumours with particular reference to melanoma.

Studium buněčné odpovědi na poškození DNA a indukce p53 u lidských nádorů se zvláštním zřetelem na melanom.

• MeSH
• geny p53 MeSH
• monoklonální protilátky MeSH
• nádorový supresorový protein p53 MeSH
• regulace genové exprese MeSH
• vazba proteinů MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• biologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

• Publikační typ
• abstrakty MeSH