- Publikační typ
- abstrakt z konference MeSH
- MeSH
- aplikace inhalační MeSH
- buněčná imunita * MeSH
- imunotoxiny MeSH
- mitogeny MeSH
- modely nemocí na zvířatech MeSH
- nádory nosu chemicky indukované MeSH
- nádory plic chemicky indukované MeSH
- nanočástice * MeSH
- nikl * imunologie toxicita MeSH
- potkani Sprague-Dawley MeSH
- pracovní expozice MeSH
- proliferace buněk * MeSH
- slezina cytologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- techniky in vitro MeSH
- MeSH
- buňky K562 MeSH
- dieta vegetariánská * MeSH
- klinické zkoušky jako téma MeSH
- lidé MeSH
- mladý dospělý MeSH
- mononukleární fagocytární systém * MeSH
- přirozená imunita * MeSH
- průtoková cytometrie MeSH
- senioři MeSH
- Check Tag
- lidé MeSH
- mladý dospělý MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- barvicí látky * analýza škodlivé účinky toxicita MeSH
- dinitrobenzeny * chemie MeSH
- inbrední kmeny potkanů MeSH
- kontaktní dermatitida * imunologie MeSH
- test regionální lymfatické uzliny * MeSH
- textilie * škodlivé účinky toxicita MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Genotoxic effects related to exposure to styrene have been a matter of investigation for many years by employing markers of exposure, effect and susceptibility. The role of individual DNA-repair capacity in response to exposure to styrene may explain the controversial results so far obtained, but it is still scarcely explored. In the present study, we measured capacity to repair oxidative DNA damage in cell extracts obtained from 24 lamination workers occupationally exposed to styrene and 15 unexposed controls. The capacity to repair oxidative DNA damage was determined by use of a modified comet assay, as follows: HeLa cells, pre-treated with photosensitizer and irradiated with a halogen lamp in order to induce 7,8-dihydroxy-8-oxoguanine, were incubated with cell extracts from mononuclear leukocytes of each subject. The level of strand breaks reflects the removal of 7,8-dihydroxy-8-oxoguanine from substrate DNA by the enzymatic extract. In styrene-exposed subjects a moderate, non-significant increase in oxidative DNA repair was observed. Stratification for sex and smoking habit showed that unexposed males (P=0.010) and unexposed smokers (P=0.037) exhibited higher DNA-repair rates. The repair capacity did not correlate with parameters of styrene exposure and biomarkers of genotoxic effects (DNA strand breaks, N1-styrene-adenine DNA adducts, chromosomal aberrations and mutant frequencies at the HPRT locus). Significantly higher levels of DNA-repair capacity were observed in carriers of GSTM1-plus, compared to those with a deletion in GSTM1. The DNA-repair capacity was significantly lower in individuals with variant Gln/Gln genotype in XRCC1 Arg399Gln than in those with heterozygous Arg/Gln and wild-type Arg/Arg genotypes. Significantly lower repair capacity was also found in individuals with the wild-type Lys/Lys genotype in XPC Lys939Gln as compared with those homozygous for the Gln/Gln variant genotype.
- MeSH
- biologické markery * analýza MeSH
- dospělí MeSH
- genotyp MeSH
- guanin * analogy a deriváty metabolismus MeSH
- HeLa buňky MeSH
- kometový test MeSH
- lidé středního věku MeSH
- lidé MeSH
- náchylnost k nemoci MeSH
- oprava DNA * MeSH
- polymorfismus genetický MeSH
- poškození DNA MeSH
- pracovní expozice * MeSH
- styren * toxicita MeSH
- testy genotoxicity MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
Male Sprague-Dawley rats were treated by intratracheal instillation with 1 mg/animal of refractory ceramic fibers. Intratracheal exposure to ceramic fibers led to significant changes of immune response. Results of proliferative activity of spleen lymphocytes showed significantly decreased proliferative activity of T-cells in response to mitogens phytohemagglutinin and concanavalin A in animals given ceramic fibers in comparison with control rats. Similarly, T-dependent B-cell response to pokeweed mitogen was significantly suppressed. Spontaneous proliferative activity of lymphocytes in non-stimulated spleen cell cultures did not differ in exposed and control rats. No significant changes were found among groups in percentage of phagocytic blood polymorphonuclear leukocytes and percentage of cells with respiratory burst.
- MeSH
- fagocytóza MeSH
- kaolin toxicita MeSH
- keramika MeSH
- krysa rodu rattus MeSH
- lymfocyty imunologie MeSH
- minerální vlákna toxicita MeSH
- potkani Sprague-Dawley MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- antitumorózní látky farmakologie imunologie škodlivé účinky MeSH
- B-lymfocyty cytologie imunologie účinky léků MeSH
- butyráty farmakologie imunologie škodlivé účinky MeSH
- experimentální nádory farmakoterapie MeSH
- fibrosarkom farmakoterapie MeSH
- isothiokyanatany farmakologie MeSH
- krysa rodu rattus MeSH
- tělesná hmotnost účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
