UNLABELLED: Gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine resulting in significant reductions in yield and fruit quality. In order to examine the molecular mechanisms that characterize the interaction between B. cinerea and the host plant, the grapevine cytoplasmic proteome was analyzed by two-dimensional polyacrylamide gel electrophoresis. The interaction between Vitis vinifera cv. Gamay cells and B. cinerea was characterized by the increase in spot abundance of 30 proteins, of which 21 were successfully identified. The majority of these proteins were related to defence and stress responses and to cell wall modifications. Some of the modulated proteins have been previously found to be affected by other pathogens when they infect V. vinifera but interestingly, the proteins related to cell wall modification that were influenced by B. cinerea have not been shown to be modulated by any other pathogen studied to date. Transcript analysis using the quantitative real time polymerase chain reaction additionally revealed the up-regulation of several acidic, probably extracellular, chitinases. The results indicate that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are likely to be important mechanisms in the defence of grapevine against B. cinerea. BIOLOGICAL SIGNIFICANCE: Although gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine, little information is available about proteomic changes in this pathosystem. These results suggest that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are important molecular mechanisms in the defence of grapevine against B. cinerea. Surprisingly, the proteins related to cell wall modification that were modulated by B. cinerea have not been shown to be affected by any other pathogen studied to date.
- Publication type
- Meeting Abstract MeSH
- Publication type
- Meeting Abstract MeSH
Autoři referují případ dramaticky se rozvíjející nekrotizující fasciitidy s následným multiorgánovým selháním způsobený raritním agens Vibrio vulnificus.
The authors report a rare case of necrotizing fasciitis, sepsis caused by a strain of Vibrio vulnificus rare in Czech Republic.
- MeSH
- Amputation, Surgical methods MeSH
- Fasciitis, Necrotizing etiology surgery mortality MeSH
- Vibrio Infections microbiology mortality pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Multiple Organ Failure MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
Cytostatikum 5-fluorouracil (5-FU) je v současné době používáno v pooperační léčbě glaukomu. V letech 1993-6 jsme na Oční klinice v Hradci Králové subkon- junktiválně aplikovali 5-FU v souboru 158 očí po trabekulektomii (TE). Základní indikací bylo zarůstání filtračního puchýřku nebo vzestup nitrooční tenze na 20 torr a více u 148 očí. Preventivně byl 5-FU podán peroperačně při revizích TE u 10 očí. V uvedeném souboru s nepříznivým průběhem pooperačního jizvení rány jsme tak dosáhli ve sledovaném období 2 let po operaci normalizace NT do 16 torrů bez další léčby (hodnotu a stav bez léčby považujeme za optimální stav) v 37 % případů a v 81 % do 20 torr s léčbou. Vedlejší účinky podání farmaka (nejčastěji eroze epitelu rohovky) byly přechodné. Pro zmíněné indikace se 5-FU jeví jako cytostatikum s možností exaktního dávkování, dostatečnou bezpečností aplikace i optimálním účinkem.
The cytostatic 5-fluorouracil (5-FU) is used at present in the postoperative treat- ment of glaucoma. In 1993-6 we administered by the subconjunctival route at the Ophthalmological Clinic in Hradec Králové 5-FU to a group of 158 eyes after trabeculectomy (TE). The basic indication was ingrowth of the filtration vesicle or a rise of intraocular tension to 20 torr and more in 148 eyes. Preventively 5-FU was administered during operation during revisions of TE in 10 eyes. In the mentioned group with an unfavourable course of postoperative scar formation in the wound we achieved during the investigation period of two years after surgery normalization of the intraocular pressure to less than 16 torr without further treatment (we consider the value and condition without treatment as the optimal condition) in 37% of cases and in 81% less than 20 torr with treatment.Side-effects of administration of 5-FU (most frequently erosion of the corneal epithelium were transient. For the mentioned indications 5-FU appears, being a cytostatic where the dosage can be exact, sufficiently safe for administration and it exerts an optimal effect.
- MeSH
- Adult MeSH
- Research Support as Topic MeSH
- Fluorouracil administration & dosage adverse effects therapeutic use MeSH
- Injections methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Intraocular Pressure MeSH
- Preoperative Care MeSH
- Antineoplastic Agents administration & dosage therapeutic use MeSH
- Aged MeSH
- Trabeculectomy methods MeSH
- Treatment Outcome MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Review MeSH
- Comparative Study MeSH
