OBJECTIVES: Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage. METHODS: First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification ("NON"), acidification before storage ("PRE"), or acidification after storage ("POST"). The analyses were conducted on the day of arrival (day 0, "baseline"), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as ±20 % from the baseline. RESULTS: The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine. CONCLUSIONS: Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.

• MeSH
• analýza moči metody   MeSH
• dospělí MeSH
• koncentrace vodíkových iontů MeSH
• kyselina močová moč   MeSH
• lidé středního věku MeSH
• lidé MeSH
• močové kameny * moč   MeSH
• odběr biologického vzorku metody   MeSH
• recidiva * MeSH
• sbírání vzorku moči metody   MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Introduction: Kidney stone formers can have higher oxalate and phosphate salt amounts in their urine than healthy people and we hypothesized that its acidification may be useful. The study aims to compare results of urine concentrations of calcium, magnesium, and inorganic phosphorus in the midstream portion of first voided morning urine samples without (FMU) and with post-collection acidification (FMUa) in kidney stone patients. Materials and methods: This is a prospective single center study. A total of 138 kidney stone patients with spot urine samples were included in the study. Urine concentrations of calcium, magnesium and inorganic phosphorus were measured with and without post-collection acidification. Acidification was performed by adding 5 μL of 6 mol/L HCl to 1 mL of urine. Results: The median age (range) of all participants was 56 (18-87) years. The median paired differences between FMU and FMUa concentrations of calcium, magnesium, and inorganic phosphorus were: - 0.040 mmol/L, 0.035 mmol/L, and 0.060 mmol/L, respectively. They were statistically different: P < 0.001, P < 0.001, P = 0.004, respectively. These differences are not clinically significant because biological variations of these markers are much higher. Conclusions: No clinically significant differences in urinary calcium, magnesium, and inorganic phosphorus concentrations between FMU and FMUa in patients with kidney stones were found.

• MeSH
• elektrolyty MeSH
• fosfor MeSH
• hořčík * moč   MeSH
• koncentrace vodíkových iontů MeSH
• ledvinové kameny * MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• vápník MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Informační příručka pro pacienty s lymfomem. Poskytuje rady ohledně rekonvalecence, zdravého životního stylu, psychického zdraví, poléčebných komplikacích, sociálně-právních otázek.

• MeSH
• balneologie MeSH
• farmakoterapie MeSH
• fertilita MeSH
• lymfom psychologie   rehabilitace   MeSH
• nežádoucí účinky léčiv MeSH
• rekonvalescence MeSH
• sociální zabezpečení MeSH
• zdravé chování MeSH
• Publikační typ
• populární práce MeSH
• příručky MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• onkologie
• hematologie a transfuzní lékařství

Macleaya microcarpa (Maxim.) Fedde belongs to the genus Macleaya, family Papaveraceae. Together with the better known and more frequently studied species M. cordata (Willd.) R. Br. it is a main source of quaternary benzo[c]phenanthridine alkaloids. Using HPLC we determined the content of eight isoquinoline alkaloids in the aerial and underground parts of 1-, 2-, 12- and 13-year old plants and followed their changes during the vegetative period. The dominant alkaloid of all samples collected in the end of this period was allocryptopine (3.8-13.6 mg/g for aerial parts, 24.2-48.9 mg/g for underground parts). Chelerythrine, sanguinarine and protopine were also present in both parts of the plant. Additionally, measurable concentrations of chelilutine (CL), chelirubine (CR), macarpine (MA) and sanguirubine (SR) were detected in underground parts. The most important finding was that contents of CR, CL, SR and MA in the 12- and 13-year old plant roots were significantly higher (approximately 3-fold for CR, 6-fold for CL, 5-fold for SR, and at least 14-fold for MA) than in 1- or 2-year old plants. The proportion of individual alkaloids in aerial and underground parts thus changed significantly during the vegetative period.

• MeSH
• alkaloidy chemie   MeSH
• Papaveraceae chemie   MeSH
• roční období MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The content of the seven quaternary benzo[c]phenanthridine alkaloids (QBA) sanguinarine (SA), chelerythrine (CHE), chelirubine (CHR), chelilutine (CHL), sanguilutine (SL), sanguirubine (SR) and macarpine (MA) was determined in the underground part of six plant species of the family Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) and Stylophorum lasiocarpum (Oliv.)). HPLC method with reversed phase column Synergi Max-RP C-12 Phenomenex was used, mobile phase consisted of heptanesulfonic acid (0.01 mol/l) with triethanolamine (0.1 mol/l) in redistilled water, pH 2.5, acetonitrile gradient 25-60% during 25 min. Detection was performed at 280 nm. The highest content of SA and CHE was found in the roots of D. lactucoides (1.99%, resp. 3.43% of the dried roots). In rhizomes of S. canadensis was their content more then two times lower.

• MeSH
• acetonitrily chemie   MeSH
• alkaloidy analýza   chemie   MeSH
• druhová specificita MeSH
• ethanolaminy chemie   MeSH
• fenantridiny analýza   chemie   izolace a purifikace   MeSH
• financování organizované MeSH
• kalibrace MeSH
• koncentrace vodíkových iontů MeSH
• kořeny rostlin chemie   MeSH
• léčivé rostliny anatomie a histologie   chemie   MeSH
• methanol chemie   MeSH
• molekulární struktura MeSH
• Papaveraceae anatomie a histologie   chemie   MeSH
• regresní analýza MeSH
• reprodukovatelnost výsledků MeSH
• rostlinné extrakty chemie   MeSH
• spektrofotometrie ultrafialová MeSH
• voda chemie   MeSH
• vysokoúčinná kapalinová chromatografie metody   přístrojové vybavení   MeSH

• MeSH
• farmakologie MeSH
• lidé MeSH
• reklama zaměřená na spotřebitele * zákonodárství a právo   MeSH
• Check Tag
• lidé MeSH

• MeSH
• personál nemocniční MeSH
• personální management zákonodárství a právo   MeSH
• pracovní uspokojení MeSH
• zaměstnanost MeSH

• MeSH
• exploze MeSH
• kooperační chování MeSH
• lidé MeSH
• odborný výcvik metody   MeSH
• záchranná práce metody   organizace a řízení   MeSH
• Check Tag
• lidé MeSH

• MeSH
• civilní obrana MeSH
• informační systémy MeSH
• telekomunikace MeSH

• Publikační typ
• abstrakty MeSH