Wild strains of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis were tested in an experimental hyperbaric chamber to determine the possible effect of hyperbaric oxygen on the susceptibility of these strains to the antibiotics ampicillin, ampicillin + sulbactam, cefazolin, cefuroxime, cefoxitin, gentamicin, sulfamethoxazole + trimethoprim, colistin, oxolinic acid, ofloxacin, tetracycline, and aztreonam during their cultivation at 23 °C and 36.5 °C. Ninety-six-well inoculated microplates with tested antibiotics in Mueller-Hinton broth were cultured under standard incubator conditions (normobaric normoxia) for 24 h or in an experimental hyperbaric chamber (HAUX, Germany) for 24 h at 2.8 ATA of 100% oxygen (hyperbaric hyperoxia). The hyperbaric chamber was pressurised with pure oxygen (100%). Both cultures (normoxic and hyperoxic) were carried out at 23 °C and 36.5 °C to study the possible effect of the cultivation temperature. No significant differences were observed between 23 and 36.5 °C cultivation with or without the 2-h lag phase in Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Cultivation in a hyperbaric chamber at 23 °C and 36.5 °C with or without a 2-h lag phase did not produce significant changes in the minimum inhibitory concentration (MIC) of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. For the tested strains of Pseudomonas aeruginosa, the possible effect of hyperbaric oxygen on their antibiotic sensitivity could not be detected because the growth of these bacteria was completely inhibited by 100% hyperbaric oxygen at 2.8 ATA under all hyperbaric conditions tested at 23 °C and 36.5 °C. Subsequent tests with wild strains of pseudomonads, burkholderias, and stenotrophomonads not only confirmed the fact that these bacteria stop growing under hyperbaric conditions at a pressure of 2.8 ATA of 100% oxygen but also indicated that inhibition of growth of these bacteria under hyperbaric conditions is reversible.
- MeSH
- ampicilin farmakologie MeSH
- anaerobní bakterie MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria MeSH
- Escherichia coli MeSH
- hyperbarická oxygenace * MeSH
- Klebsiella pneumoniae MeSH
- kombinace léků trimethoprim a sulfamethoxazol farmakologie MeSH
- kyslík MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- oxidační stres MeSH
- pseudomonádové infekce * MeSH
- Pseudomonas aeruginosa MeSH
- sulbaktam MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: This implementation project compared standard operating procedures, accepted preventive measures, and disinfection procedures between the initial stage of the COVID-19 pandemic (first wave: March 15 to May 31, 2020) and the later stages of the pandemic (second and third waves: September 1, 2020 to January 31, 2021). INTRODUCTION: This project sought to improve compliance with international evidence-based guidelines and clinical standards for the prevention and control of COVID-19 infection during hyperbaric oxygen therapy taking into account the conditions of the local hospital. METHODS: Guided by the JBI evidence implementation framework, seven evidence-based audit criteria were developed for the prevention and control of COVID-19 infection during hyperbaric oxygen therapy. A questionnaire was used to measure compliance in baseline and follow-up audits. RESULTS: Differences between the baseline and follow-up audits were noted for criteria 6 and 7. Criterion 6 increased from 0% to 100% as the hyperbaric facility was equipped with certified ultraviolet-C radiation for air disinfection during the later period, but this equipment was not available in the initial period of the pandemic. Criterion 7 dropped from 100% in the baseline audit to 0% in the follow-up audit because of a significant increase in the operational burden of the treatment capacity of the facility, which made it impossible to comply with the recommended distancing between patients. CONCLUSIONS: Differences were found in preventive measures, disinfection procedures, work organization, and approach to care strategy. The project objectives were met and the implementation strategies proved effective. Larger sample sizes would need be needed to confirm the reproducibility of the results.
- MeSH
- COVID-19 * prevence a kontrola MeSH
- hyperbarická oxygenace * MeSH
- lidé MeSH
- nemocnice MeSH
- pandemie prevence a kontrola MeSH
- reprodukovatelnost výsledků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Jedním z výrazných ekonomických výdajů pro zdravotnická zařízení v oblasti péči o rány jsou pacienti s diabetickými defekty anebo s ischemickou chorobou dolních končetin vyššího stupně. Léčebným cílem u těchto diagnóz bývá snížení počtu amputací, zejména vysokých, radikálně ovlivňujících kvalitu života pacientů. Mezi adjuvantní léčebné metody patří i hyperbarická medicína, které vliv je již nějakou dobu prokazován a je i součástí některých doporučených postupů. Cílem této práce je představit recentní klinické systematické přehledy a aktuálně platná doporučení pro léčbu obtížně se hojících ulcerací podle 10. evropské konsensuální konference ECHM (Evropská komise pro hyperbarickou medicínu) z roku 2016. Součástí práce je přehled studií zabývajících se farmakoekonomickou nákladovostí, shrnutí nákladů na léčbu u diabetu, jeho komplikací, u pacientů po amputacích a nákladů na léčbu HBO. V závěru je vysvětlen princip a význam metody transkutánní oxymetrie a tzv. kyslíkové výzvy ve vztahu HBO a pacientů s diabetickou nohou, či ischemií.
One of the major economical expense for health providers in wound care management are patients with diabetic foot ulcer or a chronic lower limb ischemia. Main treatment goal in theese patients is usually reduction in a number of amputations, especially high-ones, deeply affecting quality of life of patients. Hyperbaric medicine is already estabilished as an adjuvant method in some of these indications. In some cases it is already part of the national recomendations for diabetic foot ulcer treatment. The aim of this work is to present recent clinical systematic reviews and currently valid recommendations for the treatment of difficult-to-heal ulcers according to the 10th European Consensus Conference ECHM (European Commission for Hyperbaric Medicine) from 2016. Part of the work is an overview of pharmacoeconomic studies, summary of treatment costs for diabetes, its complications, in patients after amputations as well as costs of HBOT. Finally, the principle and significance of the method of transcutaneous oximetry and the so-called oxygen challenge in relation to HBOT in patients with critical ischemia and diabetic foot syndrome are explained.
- Klíčová slova
- hyperbaric oxygen therapy, hyperoxia, diabetic foot ulcer, ischaemia, transcutaneous oximetry, hyperbarická oxygenoterapie, hyperoxie, diabetická noha, ischémie, transkutánní oxymetrie,
- MeSH
- diabetická noha terapie MeSH
- hojení ran MeSH
- hyperbarická oxygenace metody MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
The symbiotic relationship between intestinal microbiota and the host is a major mechanism of prevention against the development of chronic and metabolic diseases. The intestinal microbiota provides several physiological functions of the organism from the creation of a natural functional barrier with a subsequent immunostimulatory activity up to affecting the energy metabolism of the host. Disruption of physiological intestinal microbiota is reported as one of the major etiological factors of initiation and progression of colorectal carcinoma (CRC). Chronic low-grade inflammation is associated with the development of CRC, through the production of inflammatory cytokines and reactive oxygen species. CRC occurs in association with high-protein and high-fat diets in combination with low-fiber intake. The problem of intestinal dysbiosis and oncological diseases is a multidisciplinary problem and it is necessary to focus on several fields of medicine such as public health, clinical pharmacology, and internal medicine. The aim of this review is describing the role of gut dysbiosis in pathogenesis of colorectal carcinoma.
- MeSH
- cytokiny imunologie MeSH
- dieta MeSH
- dysbióza * MeSH
- gastrointestinální trakt mikrobiologie patofyziologie MeSH
- kolorektální nádory mikrobiologie patologie MeSH
- lidé MeSH
- reaktivní formy kyslíku metabolismus MeSH
- střevní mikroflóra * MeSH
- symbióza MeSH
- zánět MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
INTRODUCTION: Hyperbaric oxygen treatment (HBOT), based on inhaling pure oxygen under elevated ambient pressure, is used as adjuvant intervention to promote healing in infected wounds. Despite extensive clinical evidence of beneficial effects of HBOT in soft tissue infections the mechanism of action remains to be elucidated. The aim of this study was to evaluate the use of flow cytometry as a novel method to assess the viability of pathogenic bacteria after hyperbaric oxygen (HBO) exposure. METHODS: Bacterial strains associated with soft tissues infections: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus were exposed to oxygen at 2.8 atmospheres absolute (atm abs) (283.6 kPa) pressure for 45, 90, or 120 min, then stained with propidium iodide and thiazole orange and analysed by flow cytometry. RESULTS: Escherichia coli and Staphylococcus aureus showed no change in viability, nor morphology, the viability of Pseudomonas aeruginosa reduced in a dose-dependent manner and Klebsiella pneumoniae also showed dye uptake after HBO. CONCLUSIONS: These initial results, indicate diverse sensitivity of bacteria to HBO, and suggest that flow cytometry can be used to monitor viability and morphological changes triggered by HBO exposure in bacteria.
Indikační seznam onemocnění vhodných k léčbě hyperbarickým kyslíkem se vyvíjí zejména v souvislosti s vývojem vědeckých důkazů. Evropský výbor pro hyperbarickou medicínu (European Committee for Hyperbaric Medicine, ECHM) je zaměřen na podporu neustálého zlepšování kvality péče a bezpečnosti v hyperbarické medicíně. Od roku 1994 se uskutečnilo deset konferencí, vydávajících doporučení zasahující širokou odbornou veřejnost. Poslední, 10. konsenzuální konference ECHM se uskutečnila v dubnu 2016 v Lille. Byly předloženy zprávy, vypracované členy odborného panelu, složeného z uznávaných odborníků v každé oblasti. Tyto byly založené na důkladném prozkoumání dostupné literatury a syntéze důkazů, s následným navržením doporučení podle systému GRADE (Grading of Recommendations, Assessment, Development and Evaluation System). V průběhu konference byly zprávy a znalecké posudky prezentovány a diskutovány. Publikum pak hlasovalo o každém doporučení, přičemž výsledky hlasování byly dokumentovány a na základě shody mezi vědeckými důkazy a výsledky hlasování publika byla vydána závěrečná konsenzuální doporučení. V práci je předložen a analyzován aktuální přehled doporučení podle 10. konsenzuální konference ECHM z roku 2016. Došlo zde k rozšíření doporučeného seznamu o některé indikace, které byly dříve řazeny mezi nedoporučované indikace, u některých indikací došlo ke zvýšení síly a změně typu doporučení.
A list of diseases suitable for hyperbaric oxygen therapy is evolving particularly in the context of the development of scientific evidence. The European Committee for Hyperbaric Medicine (ECHM) aims to promote continuous improvement of the quality of care and safety in hyperbaric medicine. Since 1994, ten conferences have been held, delivering recommendations to the broad professional public. The last, 10th ECHM Consensus Conference was held in Lille in April 2016. Reports were introduced, drawn up by panel members, composed of recognized experts in each area. These were based on a thorough examination of the available literature and the synthesis of evidence, followed by the recommendation of GRADE system (Grading of Recommendations, Assessment, Development and Evaluation System). During the conference the reports and expert opinions were presented and discussed. The audience then voted on each recommendation. The voting results were documented and a final consensual recommendation was issued on the basis of the consensus between scientific evidence and the results of the vote of the audience. The current overview of recommendations according to the 10th ECHM Consensual Conference of 2016 is presented and analysed in this paper. The recommended list has been expanded by some indications that were previously listed among the non-recommended indications, some indications have been increasing strength and changing the type of recommendation.
- MeSH
- hodnocení postupů (zdravotní péče) MeSH
- hyperbarická oxygenace * MeSH
- lidé MeSH
- medicína založená na důkazech MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
- směrnice pro lékařskou praxi MeSH
The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1-6) and strains isolated from colorectal carcinoma diagnoses (strains 7-8; Wilcoxon non-parametric test p = 0.0132; t test = 0.1110; t test with log-transformed data, p value = 0.0294).
- MeSH
- Bacteroides fragilis chemie metabolismus MeSH
- endotoxiny metabolismus MeSH
- hyperbarická oxygenace MeSH
- kolorektální nádory mikrobiologie terapie MeSH
- kyslík * MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Prístrojové potápanie vyvoláva v organizme mnoho fyziologických zmien, najmä v kardiovaskulárnom, respiračnom a nervovom systéme. Vzhľadom na technické obmedzenia pri pobyte človeka pod vodnou hladinou, resp. v hyperbarickom prostredí, sa výskumné aktivity prevažne uskutočňujú pred a po ponore, čo značne limituje naše vedomosti o zmenách prebiehajúcich v reálnom čase v organizme potápača počas ponoru. Cieľom projektu bolo otestovať možnosti telemetrického sledovania potkanov počas ponorov v experimentálnej hyperbarickej komore. V našej práci sme zistili, že je možné telemetricky merať srdečnú variabilitu u potkanov počas ponorov do pabs 300 kPa. Pilotné výsledky naznačujú, že kyslík zohráva kľučovú úlohu v prispôsobení sa organizmu hyperbarickej hyperoxii.
SCUBA diving is associated with significant changes in human physiology. The most influenced organ systems are the cardiovascular, respiratory and nervous system. Traditionally, the diver is monitored pre and post dive, since environmental conditions while diving represents the main technological constraints for real-time measurements. These, together with the complexity of triggered physiological changes, limit our deep understanding of the effect of scuba diving on a diver’s physiology. The aim of this study was to develop and test telemetric measurement of heart rate and heart rate variability (HRV) in un-anesthetized conscious rats during experimental dives in the experimental hyperbaric chamber. Our pilot study showed the feasibility of telemetric measurement of heart rate and HRV up to pabs 300 kPa. Data indicate, that oxygen sensing might play a more significant role in cardiovascular adjustment to hyperbaric hyperoxia, however, more experiments are needed to fully understand underlying mechanisms.
- Klíčová slova
- hyperbarická komora,
- MeSH
- baroreflex MeSH
- hyperoxie * MeSH
- pilotní projekty MeSH
- potápění fyziologie MeSH
- potkani Wistar MeSH
- srdeční frekvence MeSH
- telemetrie MeSH
- určení tepové frekvence * metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
