The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydroperoxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at -20 and -80 °C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients >0.9. The TTL assay showed good results in serum samples stored at -80 °C, but not in samples stored at -20 °C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at -80 °C. ROM and BAP can also be stored at -20 °C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions.
- MeSH
- Biomarkers blood MeSH
- Humans MeSH
- Oxidative Stress * MeSH
- Serum metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Publication type
- Meeting Abstract MeSH
- Publication type
- Meeting Abstract MeSH
In coronary heart disease, the treatment of significant stenosis by percutaneous coronary intervention (PCI) with stent implantation elicits local and systemic inflammatory responses. This study was aimed at evaluation of the dynamics of inflammatory response and elucidation of the relationship between the fatty acid profile of red blood cell (RBC) membranes or plasma phospholipids and inflammation after PCI. High-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), serum amyloid A (SAA), malondialdehyde (MDA) and the fatty acid profiles were determined in patients with advanced coronary artery disease undergoing PCI before, 24 h and 48 h after drug-eluting stent implantation (n=36). Patients after PCI exhibited a significant increase in studied markers (hsCRP, IL-6, SAA, MDA). Many significant associations were found between the increase of IL-6, resp. SAA and the amounts of n-6 polyunsaturated fatty acids (namely linoleic, dihomo-gamma-linolenic, docosatetraenoic and docosapentaenoic acid), resp. saturated fatty acids (pentadecanoic, stearic, nonadecanoic) in erythrocyte membranes. The magnitude of the inflammatory response to PCI is related to erythrocyte membrane fatty acid profile, which seems to be a better potential predictor of elevation of inflammatory markers after PCI than plasma phospholipids.
- MeSH
- Biomarkers blood MeSH
- Erythrocyte Membrane chemistry MeSH
- Phospholipids blood MeSH
- Percutaneous Coronary Intervention MeSH
- Humans MeSH
- Malondialdehyde blood MeSH
- Fatty Acids blood MeSH
- Coronary Artery Disease blood surgery MeSH
- Fatty Acids, Unsaturated blood MeSH
- Oxidative Stress MeSH
- Postoperative Complications blood MeSH
- Cross-Sectional Studies MeSH
- Aged MeSH
- Stents adverse effects MeSH
- Inflammation blood etiology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
30 stran : ilustrace, tabulky ; 30 cm
- MeSH
- Economics, Pharmaceutical MeSH
- Drug Evaluation MeSH
- Pharmaceutical Preparations MeSH
- Registries MeSH
- Terminology as Topic MeSH
- Legislation, Drug MeSH
- Health Policy MeSH
- Publication type
- Handbook MeSH
- Terminology MeSH
- Geographicals
- Czech Republic MeSH
- Conspectus
- Farmacie. Farmakologie
- NML Fields
- farmacie a farmakologie
20 stran : ilustrace ; 30 cm
- MeSH
- Economics, Pharmaceutical MeSH
- Drugs, Generic MeSH
- Pharmaceutical Preparations MeSH
- Drug Prescriptions MeSH
- Nonprescription Drugs MeSH
- Drug Costs MeSH
- Legislation, Drug MeSH
- Insurance, Health MeSH
- Publication type
- Review MeSH
- Terminology MeSH
- Geographicals
- Czech Republic MeSH
- Europe MeSH
- Conspectus
- Farmacie. Farmakologie
- NML Fields
- farmacie a farmakologie
- NML Publication type
- informační publikace
- MeSH
- Drugs, Generic * economics therapeutic use supply & distribution MeSH
- Drug Prescriptions economics MeSH
- Drug Approval * economics legislation & jurisprudence MeSH
- Universal Health Insurance economics legislation & jurisprudence MeSH
- Legislation, Drug * economics MeSH
- Publication type
- Interview MeSH
