BACKGROUND: Catheter ablation (CA) of atrial fibrillation (AF) is indicated in patients with recurrent and symptomatic AF episodes. Despite the strict inclusion/exclusion criteria, AF recurrence after CA remains high. Identification of a novel biomarker that would predict AF recurrence would help to stratify the patients. The aim of the study was to seek novel biomarkers among the plasmatic microRNAs (miRNAs, miRs). METHODS: A prospective monocentric study was conducted. A total of 49 consecutive AF patients indicated for CA were included. Blood sampling was performed prior to CA. RNA was isolated from plasma using commercial kits. In the exploration phase, small RNA sequencing was performed in ten AF patients (five with and five without AF recurrence) using Illumina instrument. In the validation phase, levels of selected miRNAs were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in all participants. RESULTS: Altogether, 22 miRNAs were identified as altered between the groups by next-generation sequencing (using the DESeq2 algorithm). Using qRT-PCR, levels of the five most altered miRNAs (miR-190b/206/326/505-5p/1296-5p) were verified in the whole cohort. Plasma levels of hsa-miR-206 were significantly higher in patients with early (within 6 months) AF recurrence and showed an increase of risk recurrence,2.65 times by every increase in its level by 1 unit in the binary logistic regression. CONCLUSION: We have identified a set of 22 plasmatic miRNAs that differ between the patients with and without AF recurrence after CA and confirmed hsa-miR-206 as a novel miRNA associated with early AF recurrence. Results shall be verified in a larger independent cohort.
- MeSH
- biologické markery * krev MeSH
- fibrilace síní * genetika krev diagnóza chirurgie MeSH
- katetrizační ablace * škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA * krev genetika MeSH
- prognóza MeSH
- prospektivní studie MeSH
- recidiva * MeSH
- senioři MeSH
- vysoce účinné nukleotidové sekvenování * MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cílem studie bylo zjistit teplotní odezvu rukou na práci na počítači v reálném pracovním prostředí. Také analyzovat vztahy mezi touto odezvou a věkem, pohlavím a subjektivními zdravotními problémy subjektů. To vše odděleně pro pravou, levou i obě ruce dohromady. Teplota byla měřena termokamerou FLIR P660. Měření proběhlo na 26 osobách (věk: 34,4 SD = 10.9 let, 21 žen, 5 mužů). Po měření teplotní odezvy v průběhu pracovní směny byly z naměřených teplot určeny ukazatele popisující dynamiku těchto změn, jako maximální teplota za celou směnu (max), minimální (min), průměrná změna teplot mezi jednotlivými měřeními (průměr dif.) a jiné. I přes rozdílné zapojení pravé a levé ruky a přes rozdílnost pohlaví nebyly mezi jejich odezvou pozorovány statisticky významné rozdíly. Jisté statisticky významné rozdíly (p = 0,039) byly naměřeny při porovnání skupiny subjektů pracujících a nepracujících na osobním počítači. Statisticky významné rozdíly (p = 0,041) a rozdíly s tendencí ke statistické významnosti byly u některých ukazatelů nalezeny mezi subjekty se zdravotními obtížemi připisovanými zaměstnání a skupinou bez obtíží. Porovnání měřených ukazatelů u skupin rozdělených podle věku přineslo pouze nevýraznou tendenci ke statistické významnosti (p = 0,082) u jediného ukazatele. Ukazatele s tendencí ke korelaci byly nalezeny v závislosti na věku měřené osoby (p = 0,058). Vzhledem k malému počtu měřených osob může být interpretace výsledků složitá. Doporučujeme proto další výzkum v této oblasti. Ten by mohl vést ke změnám diagnostiky onemocnění rukou při práci nebo k zavedení efektivnějších pracovně-hygienických standardů.
The aim of the study was to determine the temperature response of hands to work on a computer in a real work environment. To analyse the relationships between this response and age, gender, and subjective health problems of measured subjects. All this separately for the right, left and both hands together. The temperature was measured with a FLIR P660 thermal camera. The measurement was performed on 26 persons (age: 34.4 years SD = 10.9 years, 21 women, 5 men). After measuring the temperature response during the work shift, indicators describing the dynamics of these changes were determined from the measured temperatures, such as maximum temperature for the whole shift (max), minimum (min), average temperature change between individual measurements (prumer dif) and others. Despite the different involvement of the right and left hands and the difference in gender, no statistically significant differences were observed between their responses. Certain statistically significant (p = 0.039) differences were measured when comparing the group of subjects working and not working on a personal computer. Statistically significant differences and differences with a tendency to statistical significance (p = 0.041) were found for some indicators between subjects with health difficulties attributed to employment and the group without difficulties. The comparison of measured indicators in the groups divided by age showed only a slight tendency to statistical significance (p = 0.082) for a single indicator. Indicators with a tendency to correlate were found depending on the age of the measured person (p = 0.058). Due to the small number of measured people, the interpretation of the results can be difficult. Therefore, we recommend further research in this area. This could lead to changes in the diagnosis of hand diseases at work or to the introduction of more effective occupational hygiene standards.
- Klíčová slova
- práce na počítači, teplota rukou,
- MeSH
- dospělí MeSH
- klinická studie jako téma MeSH
- lidé MeSH
- počítače MeSH
- práce * MeSH
- ruka fyziologie MeSH
- teplota MeSH
- termografie * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Diabetes mellitus 2 (DM2) is the seventh cause of death worldwide. One of the reasons is late diagnosis of vascular damage. Pulse wave velocity (PWV) has become an independent marker of arterial stiffness and cardiovascular risk. Moreover, the previous studies have shown the importance of beat-to-beat PWV measurement due to its variability among the heart cycle. However, variability of PWV (PWVv) of the whole body hasn't been examined yet. We have studied a group of DM II and heathy volunteers, to investigate the beat-to-beat mean PWV (PWVm) and PWVv in the different body positions. PWV of left lower and upper extremities were measured in DM2 (7 m/8 f, age 68+/-10 years, BP 158/90+/-19/9 mm Hg) and healthy controls (5 m/6 f, age 23+/-2 years, BP 117/76+/-9/5 mm Hg). Volunteers were lying in the resting position and of head-up-tilt in 45° (HUT) for 6 min. PWVv was evaluated as a mean power spectrum in the frequency bands LF and HF (0.04-0.15 Hz, 0.15-0.5 Hz). Resting PWVm of upper extremity was higher in DM2. HUT increased lower extremity PWVm only in DM2. Extremities PWVm ratio was significantly lower in DM2 during HUT compared to controls. LF and HF PWVv had the same response to HUT. Resting PWVv was higher in DM2. Lower extremity PWVv increased during HUT in both groups. PWVm and PWVv in DM2 differed between extremities and were significantly influenced by postural changes due to hydrostatic pressure. Increased resting PWVm and PWVv in DM2 is a marker of increased arterial stiffness.
- MeSH
- analýza pulzové vlny metody MeSH
- diabetes mellitus 2. typu metabolismus patologie MeSH
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- nemoci cév diagnóza metabolismus patologie MeSH
- postura těla fyziologie MeSH
- rychlost toku krve fyziologie MeSH
- senioři MeSH
- srdeční frekvence fyziologie MeSH
- studie případů a kontrol MeSH
- tuhost cévní stěny MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Nutritional status, its assessment and its influence on numerous diseases is currently still being discussed. The aim of this study was to determine the current state of nutrition by various research methods in school children. Also, to verify the accuracy of body mass index (BMI) determination in school environment and to compare the nutritional status in two different types of school. We used a quantitative questionnaire method in 64 children from schools in the South Moravian Region. We have verified the weight and height measurement in the same group by sophisticated instruments. We have determined the basic parameters of the cardiovascular system (non-invasive continuously blood pressure recording; mutual spectral analysis for estimation of baroreflex sensitivity; applanation tonometry and cardiac polygraphy for arterial stiffness evaluation). The indicative questionnaire method encountered a problem with the weight of almost 40 % of the population approached - both obesity (14.3 %) and malnutrition (25.2 %). Cardiovascular parameters were in physiological range for the given age categories. We found increased values in BMI, % of body fat and heart rate in children from countryside type of school, respectively. We concluded that obesity/malnutrition are both common phenomena in the children population in the Czech Republic.
- MeSH
- cévní index srdce-kotník MeSH
- krevní tlak * MeSH
- lidé MeSH
- městské obyvatelstvo statistika a číselné údaje MeSH
- mladiství MeSH
- nutriční stav * MeSH
- obezita epidemiologie patofyziologie MeSH
- pilotní projekty MeSH
- podvýživa epidemiologie patofyziologie MeSH
- tuhost cévní stěny * MeSH
- venkovské obyvatelstvo statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Publikační typ
- abstrakt z konference MeSH
Anthracyclines represent one of the important classes of anti-cancer drugs; however, their major disadvantage is their profound cardiovascular toxicity. This study aimed to evaluate influence of anthracyclines on cardiovascular stiffness parameters estimated from pulse wave (PW). PW was measured in 59 cancer survivors treated with anthracyclines in childhood and in 248 healthy age-matched controls. Both patients and controls were divided into three age groups (13 - 15, 16 - 18 and 19 - 24 years). Central PW augmentation index (C-AI75) and augmentation pressure (C-AP75), both normalized to heart rate 75 bpm, were calculated as parameters of arterial wall stiffness. Central Buckberg sub-endocardial viability ratio (SEVR) was calculated as a parameter of diastolic function. Patients and controls were compared in each age group. C-AI75 and C-AP75 were significantly increased in patients in age groups 16 - 18 and 19 - 24 years. SEVR was decreased in patients in the oldest age group. Our results suggest that although toxic influence of anthracyclines to arterial wall and heart are developing during childhood and puberty, they can be detected rather in the adulthood. These changes are yet subclinical; however, their presence indicates potentially increased cardiovascular risk in childhood cancer survivors treated with anthracyclines during childhood.
- MeSH
- analýza pulzové vlny metody MeSH
- antracykliny škodlivé účinky MeSH
- krevní tlak účinky léků fyziologie MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nemoci cév chemicky indukované diagnóza patofyziologie MeSH
- přežívající onkologičtí pacienti * MeSH
- protinádorová antibiotika škodlivé účinky MeSH
- srdeční frekvence účinky léků fyziologie MeSH
- tuhost cévní stěny fyziologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The level of spinal cord injury (SCI) affects baroreflex regulation of blood pressure. While a parasympathetic cardiac chronotropic effect is preserved, baroreflex response could be impaired by sympathetic dysfunction under the SCI level. This study was aimed to evaluate the baroreflex function in SCI patients by the analysis of causal interaction between systolic blood pressure (SBP) and inter-beat intervals (IBI). Blood pressure was continuously recorded in 13 cervical SCI patients (CSCI), nine thoracic SCI (ThSCI) and 13 able-bodied controls (Con) during two phases: sitting (PS) and orthostatic challenge (PO). Beat-to-beat SBP and IBI sequences were obtained from continuous blood pressure recording. Closed loop of SBP-IBI interaction was mathematically opened by bivariate autoregressive model; causal coherence and baroreflex sensitivity (BRS) were calculated in baroreflex direction. Coherence quantifies causal synchronicity between SBP and IBI. The gain of transfer function from SBP to IBI represents BRS. PS (medians of CSCI/ThSCI/Con) coherence was 0.28/0.33/0.25 (no significant difference) and PS BRS was 6.98/7.54/6.66 (no difference). PO coherence was 0.18/0.58/0.45 (CSCI < ThCSI and Con; p < 0.01) and PO BRS was 2.38/5.87/6.22 (CSCI < ThCSI and Con; p < 0.01). For position change effect, there was no change in CSCI coherence; for ThSCI and Con, PS < PO (p < 0.05). For BRS in the CSCI group, PS < PO (p < 0.01); for ThSCI and Con, there was no change. BRS and coherence correlated negatively with SCI level (p < 0.01). In conclusion, baroreflex dysfunction in SCI patients was detected using causal analysis methods during orthostatic challenge only. Baroreflex dysfunction is probably an important mechanism of the more expressed blood pressure decrease associated with CSCI. The severity of autonomic dysfunction was related to SCI level.
- MeSH
- baroreflex fyziologie MeSH
- dospělí MeSH
- krevní tlak fyziologie MeSH
- lidé MeSH
- poranění míchy patofyziologie MeSH
- postura těla fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
Systolic blood pressure (SBP) changes control the cardiac inter-beat intervals (IBI) duration via baroreflex. Conversely, SBP is influenced by IBI via non-baroreflex mechanisms. Both causal pathways (feedback - baroreflex and feedforward - non-baroreflex) form a closed loop of the SBP - IBI interaction. The aim of this study was to assess the age-related changes in the IBI - SBP interaction. We have non-invasively recorded resting beat-to-beat SBP and IBI in 335 healthy subjects of different age, ranging from 11 to 23 years. Using a linear autoregressive bivariate model we obtained gain (Gain(SBP,IBI), used traditionally as baroreflex sensitivity) and coherence (Coh(SBP,IBI)) of the SBP-IBI interaction and causal gain and coherence in baroreflex (Gain(SBP->IBI), Coh(SBP->IBI) and coherence in non-baroreflex (Coh(IBI->SBP)) directions separately. A non-linear approach was used for causal coupling indices evaluation (C(SBP->IBI), C(IBI->SBP)) quantifying the amount of information transferred between signals. We performed a correlation to age analysis of all measures. Coh(IBI->SBP) and C(IBI->SBP) were higher than Coh(SBP->IBI) and C(SBP->IBI), respectively. Gain(SBP,IBI) increased and Coh(SBP->IBI) decreased with age. The coupling indices did not correlate with age. We conclude that the feedforward influence dominated at rest. The increase of Gain(SBP,IBI) with age was not found in the closed loop model. A decrease of Coh(SBP->IBI) could be related to a change in the cardiovascular control system complexity during maturation.
- MeSH
- baroreflex * MeSH
- dítě MeSH
- krevní tlak * MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- srdce fyziologie MeSH
- systola MeSH
- vývoj mladistvých * MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The aim of this study was to obtain a detailed analysis of the relationship between the finger arterial compliance C [ml/mm Hg] and the arterial transmural pressure P(t) [mm Hg]. We constructed a dynamic plethysmograph enabling us to set up a constant pressure P(css) [mm Hg] and a superimposed fast pressure vibration in the finger cuff (equipped with a source of infra-red light and a photoelectric sensor for the measurement of arterial volume). P(css) could be set on the required time interval in steps ranging between 30 and 170 mm Hg, and on sinusoidal pressure oscillation with an amplitude P(ca) (2 mm Hg) and a frequency f (20, 25, 30, 35, 40 Hz). At the same time continuous blood pressure BP was measured on the adjacent finger (Portapres). We described the volume dependence of a unitary arterial length on the time-varying transmural pressure acting on the arterial wall (externally P(css)+P(ca).sin(2pif), internally BP) by a second-order differential equation for volume. This equation was linearized within a small range of selected BP. In the next step, a Fourier transform was applied to obtain the frequency characteristic in analytic form of a complex linear combination of frequency functions. While series of oscillations [P(ca), f] were applied for each P(css), the corresponding response of the plethysmogram was measured. Amplitude spectra were obtained to estimate coefficients of the frequency characteristic by regression analysis. We determined the absolute value: elastance E, and its inverse value: compliance (C=1/E). Then, C=C(P(t)) was acquired by applying sequences of oscillations for different P(css) (and thus P(t)) by the above-described procedure. This methodology will be used for the study of finger arterial compliance in different physiological and pathological conditions.
- MeSH
- arterie fyziologie MeSH
- diagnostické techniky kardiovaskulární * přístrojové vybavení MeSH
- krevní tlak MeSH
- lidé MeSH
- mladý dospělý MeSH
- modely kardiovaskulární MeSH
- pletysmografie metody MeSH
- poddajnost MeSH
- prsty ruky MeSH
- senioři MeSH
- vibrace MeSH
- Check Tag
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
