As the concentration of methylmercury (MeHg) in the environment is insignificant, hair can be used as a suitable matrix to estimate endogenous MeHg exposure. Avalidated analytical method with AMA 254 spectrometer was used for the determination of inorganic mercury and methylmercury species in the hair of dentists, workers in fish industry and professionally non-exposed adults. ANOVA and QC Expert software was used for statistical evaluation. The number of amalgam fillings in oral cavity, consumption of fish, gender, smoking habits and age of the subjects were taken into account. A significantly higher level of inorganic bound mercury (Hg(in)) was found in the hair of dentists. The number of amalgam fillings had a slightly significant effect on Hg(in); fish consumption had a significant influence on MeHg and slightly also on Hg(in). Other parameters were not significant.
- MeSH
- dospělí MeSH
- financování organizované MeSH
- lidé středního věku MeSH
- lidé MeSH
- methylortuťné sloučeniny analýza MeSH
- mladiství MeSH
- mladý dospělý MeSH
- monitorování životního prostředí MeSH
- pracovní expozice MeSH
- ryby MeSH
- senioři MeSH
- sladká voda MeSH
- stravovací zvyklosti MeSH
- studie případů a kontrol MeSH
- vlasy, chlupy chemie MeSH
- zubní lékaři MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Geografické názvy
- Česká republika MeSH
With the aid of in vivo whole-body counting of the rats (administered simultaneously with the radionuclides (82)Br and (24)Na) by high-resolution gamma-ray spectrometry, we extended the applicability of our experimentally proved hypothesis that the biological half-life of bromide depends on the magnitude of sodium intake rather than on the intake of chloride, as was generally assumed. Variations in the biological half-life of bromide, as a substitute for sodium, were investigated in animals situated in very different physiological states, as regards their metabolic activity.
The aim of the study was to find out whether administration of selenium (Se) will protect the immature heart against ischemia/reperfusion.The control pregnant rats were fed laboratory diet (0.237 mg Se/kg diet); experimental rats received 2 ppm Na(2)SeO(3) in the drinking water from the first day of pregnancy until day 10 post partum. The concentration of Se in the serum and heart tissue was determined by activation analysis, the serum concentration of NO by chemiluminescence, cardiac concentration of lipofuscin-like pigment by fluorescence analysis. The 10 day-old hearts were perfused (Langendorff); recovery of developed force (DF) was measured after 40 min of global ischemia. In acute experiments, 10 day-old hearts were perfused with selenium (75 nmol/l) before or after global ischemia. Sensitivity to isoproterenol (ISO, pD(50)) was assessed as a response of DF to increasing cumulative dose.Se supplementation elevated serum concentration of Se by 16%. Se increased ischemic tolerance (recovery of DF, 32.28 +/- 2.37 vs. 41.82 +/- 2.91%, P < 0.05). Similar results were obtained after acute administration of Se during post-ischemic reperfusion (32.28 +/- 2.37 vs. 49.73 +/- 4.40%, P < 0.01). The pre-ischemic treatment, however, attenuated the recovery (23.08 +/- 3.04 vs. 32.28 +/- 2.37%, P < 0.05). Moreover, Se supplementation increased the sensitivity to the inotropic effect of ISO, decreased cardiac concentration of lipofuscin-like pigment and serum concentration of NO. Our results suggest that Se protects the immature heart against ischemia/reperfusion injury. It seems therefore, that ROS may affect the function of the neonatal heart, similarly as in adults.
- MeSH
- časové faktory MeSH
- financování organizované MeSH
- ischemická choroba srdeční farmakoterapie prevence a kontrola MeSH
- kontrakce myokardu účinky léků MeSH
- krysa rodu rattus MeSH
- lipofuscin metabolismus MeSH
- oxid dusnatý krev MeSH
- perfuze MeSH
- potkani Wistar MeSH
- potravní doplňky MeSH
- reperfuzní poškození myokardu farmakoterapie prevence a kontrola MeSH
- selen farmakologie krev terapeutické užití MeSH
- srdce účinky léků MeSH
- těhotenství MeSH
- tělesná hmotnost účinky léků MeSH
- velikost orgánu účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- biologické modely MeSH
- biologický transport MeSH
- bromidy aplikace a dávkování metabolismus MeSH
- finanční podpora výzkumu jako téma MeSH
- jod antagonisté a inhibitory metabolismus MeSH
- krysa rodu rattus MeSH
- maternofetální výměna látek MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
