Microbial natural products are among the main sources of compounds used in the medical biotechnology field for the purpose of drug development. However, as antibiotic resistance in pathogenic microorganisms is known to be increasing dramatically, there exists a need to develop new antibiotics. Actinomycetia have proven to be a good source of biologically active compounds, although the rediscovery of previously known compounds significantly slows down the introduction of new antibiotics. As a consequence, increasing attention is being paid to the isolation of actinomycete strains from previously unexplored sources, which can significantly increase the likelihood of discovering new biologically active compounds. This study investigated the diversity and bioactive potential of 372 actinomycete strains isolated from the rhizosphere soil of Juniperus excelsa M. Bieb. The examined actinomycete strains belonged to 11 genera, namely, Actinoplanes, Actinorectispora, Amycolatopsis, Kribbella, Micrococcus, Micromonospora, Nocardia, Promicromonospora, Rhodococcus, Saccharopolyspora and Streptomyces. The bioactive potential of each isolated actinomycete strain was determined on the basis of its ability to produce antimicrobial metabolites against Gram-positive and Gram-negative bacteria and yeast. Some 159 strains (42.74%) exhibited antimicrobial activity against at least one of the tested microbial strains. The dereplication analysis of the extract of the Streptomyces sp. Je 1-651 strain, which exhibited strong antimicrobial activity, led to the annotation of spiramycins and stambomycins. Moreover, the phylogenetic analysis based on the 16S rRNA gene sequence of the Je 1-651 strain revealed it to be close to the S. ambofaciens.
- MeSH
- Actinobacteria * MeSH
- Actinomycetales * genetika MeSH
- antibakteriální látky farmakologie metabolismus MeSH
- antiinfekční látky * MeSH
- fylogeneze MeSH
- gramnegativní bakterie genetika MeSH
- grampozitivní bakterie MeSH
- jalovec (rod) * genetika MeSH
- půda MeSH
- půdní mikrobiologie MeSH
- rhizosféra MeSH
- RNA ribozomální 16S genetika MeSH
- Streptomyces * genetika MeSH
- Publikační typ
- časopisecké články MeSH
The natural products and conventional chemotherapeutic drugs combination can increase the efficacy of anticancer treatment through their potential synergistic effects and reduce its toxicity. The current study investigates the effect of methanolic extract from aerial parts of Juniperus excelsa on cell death activities induced by vincristine in acute lymphoblastic leukemia cells. Cytotoxic activity of J. excelsa extract and vincristine in Nalm-6 and Reh cells was determined using MTT assay and synergism was evaluated using the CompuSyn software. Apoptosis was assessed by caspase 3 activity assay and flow cytometry. The expression levels of some apoptosis-related genes, Caspase 3, BAX and BCL-2 were determined by Real-time PCR. Statistical analysis was assessed by one-way ANOVA and Tukey's tests. The combined treatment of VCR and J. excelsa extract showed a synergistic cytotoxic effect on both Nalm-6 and Reh cells at low doses of vincristine (CI < 1). J. excelsa extract also significantly increased VCR-induced apoptosis (P < 0.001). Expression of CASP3 and BAX genes were upregulated, while BCL-2 gene was downregulated in both cells (P < 0.05). Our study suggested the combined use of lower doses of VCR and J. excelsa extract promote its effects by apoptosis induction and this combination could potentially decrease the side effects of the drug.
- MeSH
- aktivace enzymů MeSH
- akutní lymfatická leukemie * farmakoterapie MeSH
- apoptóza MeSH
- exprese genu MeSH
- fytogenní protinádorové látky farmakologie toxicita MeSH
- fytoterapie MeSH
- jalovec (rod) * MeSH
- kaspasa 3 MeSH
- kolorimetrie MeSH
- kombinovaná farmakoterapie MeSH
- lidé MeSH
- nádorové buněčné linie účinky léků MeSH
- nádorové buňky kultivované účinky léků MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- protein X asociovaný s bcl-2 MeSH
- průtoková cytometrie MeSH
- rostlinné extrakty farmakologie toxicita MeSH
- synergismus léků MeSH
- viabilita buněk účinky léků MeSH
- vinkristin * farmakologie toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Akutní toxické hepatitidy mohou být následkem užívání řady léků, jsou však popsány i po bylinných přípravcích. V naší kazuistice prezentujeme případ akutní toxické hepatitidy po užívání bylinného doplňku stravy za účelem léčby psoriázy. I přes rozšířený mýtus o neškodnosti mohou bylinné přípravky stejně jako „klasické" léky vyvolávat závažné nežádoucí účinky.
- MeSH
- Agropyron škodlivé účinky MeSH
- bříza škodlivé účinky MeSH
- gama-glutamyltransferasa diagnostické užití metabolismus MeSH
- jalovec (rod) škodlivé účinky MeSH
- lékové postižení jater diagnóza etiologie terapie MeSH
- ostropestřec mariánský škodlivé účinky MeSH
- potravní doplňky využití MeSH
- psoriáza farmakoterapie MeSH
- rostlinné přípravky škodlivé účinky terapeutické užití toxicita MeSH
- Publikační typ
- kazuistiky MeSH
