Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 µM cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-C4 D.

• MeSH
• Anions analysis   MeSH
• Electric Conductivity MeSH
• Electrophoresis, Capillary methods   MeSH
• Phosphates analysis   MeSH
• Gastroesophageal Reflux diagnosis   MeSH
• Bicarbonates analysis   MeSH
• Humans MeSH
• Saliva chemistry   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

• MeSH
• Acid-Base Equilibrium physiology   MeSH
• Acidosis diagnosis   etiology   classification   physiopathology   MeSH
• Pulmonary Disease, Chronic Obstructive complications   MeSH
• Bicarbonates analysis   MeSH
• Humans MeSH
• Acid-Base Imbalance * classification   physiopathology   MeSH
• Acidosis, Respiratory diagnosis   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Case Reports MeSH
• Review MeSH

• MeSH
• Cholecystokinin diagnostic use   MeSH
• Bicarbonates analysis   MeSH
• Pancreas secretion   MeSH
• Pancreatitis physiopathology   MeSH
• Secretin diagnostic use   MeSH

• MeSH
• Bicarbonates analysis   MeSH
• Hydrogen-Ion Concentration MeSH
• Cells, Cultured MeSH
• Buffers MeSH
• Temperature MeSH

• MeSH
• Chymotrypsin metabolism   MeSH
• Adult MeSH
• Bicarbonates analysis   MeSH
• Humans MeSH
• Check Tag
• Adult MeSH
• Humans MeSH

• MeSH
• Amylases blood   MeSH
• Cholecystokinin MeSH
• Gastrectomy MeSH
• Bicarbonates analysis   MeSH
• Injections, Intravenous MeSH
• Humans MeSH
• Pancreas secretion   MeSH
• Pancreatic Juice enzymology   MeSH
• Secretin MeSH
• Check Tag
• Humans MeSH

• MeSH
• Acid-Base Equilibrium MeSH
• Time Factors MeSH
• Cholecystokinin pharmacology   MeSH
• Adult MeSH
• Potassium analysis   MeSH
• Bicarbonates analysis   MeSH
• Carbonic Anhydrases MeSH
• Humans MeSH
• Fasting MeSH
• Pancreas secretion   MeSH
• Pancreatic Juice MeSH
• Secretin pharmacology   MeSH
• Sodium analysis   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH