Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 µM cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-C4 D.
- MeSH
- Anions analysis MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Phosphates analysis MeSH
- Gastroesophageal Reflux diagnosis MeSH
- Bicarbonates analysis MeSH
- Humans MeSH
- Saliva chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Acid-Base Equilibrium physiology MeSH
- Acidosis diagnosis etiology classification physiopathology MeSH
- Pulmonary Disease, Chronic Obstructive complications MeSH
- Bicarbonates analysis MeSH
- Humans MeSH
- Acid-Base Imbalance * classification physiopathology MeSH
- Acidosis, Respiratory diagnosis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Case Reports MeSH
- Review MeSH
- MeSH
- Chymotrypsin metabolism MeSH
- Adult MeSH
- Bicarbonates analysis MeSH
- Humans MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- MeSH
- Acid-Base Equilibrium MeSH
- Time Factors MeSH
- Cholecystokinin pharmacology MeSH
- Adult MeSH
- Potassium analysis MeSH
- Bicarbonates analysis MeSH
- Carbonic Anhydrases MeSH
- Humans MeSH
- Fasting MeSH
- Pancreas secretion MeSH
- Pancreatic Juice MeSH
- Secretin pharmacology MeSH
- Sodium analysis MeSH
- Check Tag
- Adult MeSH
- Humans MeSH