Our study aimed to investigate subacute exposure to alcohol in relation to bone microstructure of mice. Animals from experimental (E) group drank a solution composed of 15 % ethanol and water for 14 days (one remodeling cycle), while those from control (C) group drank only water. In the compact bone of E group, decreased bone formation and increased porosity were observed which corresponds with lower levels of serum alkaline phosphatase and glutathione. Alcohol significantly increased sizes of primary osteon's vascular canals and decreased those of secondary osteons, Haversian canals. Relative bone volume, bone mineral density (BMD), relative bone volume without marrow cavity were also lower in E group. On the contrary, trabecular bone microstructure did not differ significantly between E and C groups. Liver function test showed higher levels of alanine aminotransferase, aspartate aminotransferase in alcohol-fed mice. Serum calcium, phosphate were significantly lower in E group. According to our study, only changes in compact bone microstructure of mice following one remodeling cycle were observed due to both direct and indirect effects of alcohol.
Prospective, controlled clinical trials in postmenopausal osteoporosis typically compare effects of an active drug with placebo in addition to vitamin D and calcium supplementation in both treatment arms. While clinical benefits are documented, the effect of this supplementation in the placebo arm and in clinical practice on bone material composition properties is unknown. The purpose of the present study was to evaluate these bone quality indices (specifically mineral/matrix, nanoporosity, glycosaminoglycan content, mineral maturity/crystallinity, and pyridinoline content) in patients that either received long-term vitamin D (400-1200IU) and calcium (1.0-1.5g) supplementation, or did not. We have analyzed by Raman microspectroscopy the bone forming trabecular surfaces of iliac crest in pre-treatment samples of a teriparatide study and the endpoint biopsies of the control arm obtained from the HORIZON trial. In general, the mineral/matrix ratio and the glycosaminoglycan (GAG) content was higher while nanoporosity, (a surrogate for tissue water content), the mineral maturity/crystallinity (MMC) and the pyridinoline (Pyd) content was lower in patients without long-term supplementation. Moreover, all indices were significantly dependent on tissue age. In conclusion, vitamin D and calcium supplementation is associated with altered mineral and organic matrix properties.
- MeSH
- Amino Acids metabolism MeSH
- Analysis of Variance MeSH
- Calcification, Physiologic drug effects MeSH
- Glycosaminoglycans metabolism MeSH
- Bone Matrix drug effects metabolism MeSH
- Humans MeSH
- Nanoparticles chemistry MeSH
- Porosity MeSH
- Osteoporosis, Postmenopausal drug therapy physiopathology MeSH
- Dietary Supplements * MeSH
- Spectrum Analysis, Raman MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Calcium pharmacology therapeutic use MeSH
- Vitamin D pharmacology therapeutic use MeSH
- Check Tag
- Humans MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Chromium * adverse effects MeSH
- Microscopy, Electron MeSH
- Bone Matrix drug effects MeSH
- Disease Models, Animal MeSH
- Periodontium * drug effects MeSH
- Periodontitis chemically induced MeSH
- Rats, Sprague-Dawley * anatomy & histology MeSH
- Alveolar Bone Loss chemically induced MeSH
- Tooth Loss chemically induced MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
