Routinely used biomarkers of bacterial etiology of infection, such as C-reactive protein and procalcitonin, have limited usefulness for evaluation of infections since their expression is enhanced by a number of different conditions. Therefore, several inflammatory cytokines and chemokines were analyzed with sera from patients hospitalized for moderate bacterial and viral infectious diseases. In total, 57 subjects were enrolled: 21 patients with community-acquired bacterial infections, 26 patients with viral infections, and 10 healthy subjects (control cohorts). The laboratory analyses were performed using Luminex technology, and the following molecules were examined: IL-1Ra, IL-2, IL-4, IL-6, IL-8, TNF- α , INF- γ , MIP-1 β , and MCP-1. Bacterial etiology of infection was associated with significantly (P < 0.001) elevated serum concentrations of IL-1Ra, IL-2, IL-6, and TNF- α in comparison to levels observed in the sera of patients with viral infections. In the patients with bacterial infections, IL-1Ra and IL-8 demonstrated positive correlation with C-reactive protein, whereas, IL-1Ra, TNF- α , and MCP-1 correlated with procalcitonin. Furthermore, elevated levels of IL-1Ra, IL-6, and TNF- α decreased within 3 days of antibiotic therapy to levels observed in control subjects. The results show IL-1Ra as a potential useful biomarker of community-acquired bacterial infection.

• MeSH
• antagonista receptoru pro interleukin 1 krev   MeSH
• bakteriální infekce krev   MeSH
• biologické markery krev   MeSH
• C-reaktivní protein metabolismus   MeSH
• chemokiny krev   MeSH
• cytokiny krev   MeSH
• dospělí MeSH
• infekce získané v komunitě krev   MeSH
• interleukin-2 krev   MeSH
• interleukin-4 krev   MeSH
• interleukin-6 krev   MeSH
• interleukin-8 krev   MeSH
• kalcitonin krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• proteinové prekurzory krev   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• TNF-alfa krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Timely knowledge of the bacterial etiology and localization of infection are important for empirical antibiotic therapy. Thus, the goal of this study was to evaluate routinely used biomarkers together with novel laboratory parameters in the diagnosis of infection. METHODS: In this prospective study, 54 adult patients with bacterial infections admitted to the Department of Infectious Diseases were included. For comparison, 27 patients with viral infections were enrolled. In these patients, white blood cell (WBC) counts, differential blood counts, serum levels of procalcitonin (PCT), IL-1β, IL-6, IL-8, IL-10, IL-12, TNF-α, IFN-γ, soluble CD14 (sCD14), heparin-binding protein (HBP), cortisol (Cort), and monocyte surface expression of TLR2, TLR4, HLA-DR, and CD14 were analyzed. Also, these biomarkers were evaluated in 21 patients with acute community-acquired bacterial pneumonia (CABP), as well as in 21 patients with pyelonephritis and urosepsis. RESULTS: The highest sensitivity and specificity (expressed as the area under the curve [AUC]) for bacterial infection were observed in serum concentration of PCT (0.952), neutrophil and lymphocyte counts (0.852 and 0.841, respectively), and serum levels of HBP (0.837), IL-6 (0.830), and Cort (0.817). In addition, the serum levels of IFN-γ and Cort were significantly higher and IL-8 levels were lower in CABP when compared to pyelonephritis or urosepsis. CONCLUSIONS: From the novel potential biomarkers, only PCT demonstrated superiority over the routine parameters in the differentiation of bacterial from viral infections. However, some of the novel parameters should be further evaluated in larger and better characterized cohorts of patients in order to find their clinical applications.

• MeSH
• bakteriální infekce krev   diagnóza   MeSH
• biologické markery MeSH
• dospělí MeSH
• infekce získané v komunitě krev   diagnóza   MeSH
• kalcitonin krev   diagnostické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• močové ústrojí mikrobiologie   MeSH
• prediktivní hodnota testů MeSH
• prospektivní studie MeSH
• proteinové prekurzory krev   diagnostické užití   MeSH
• pyelonefritida krev   diagnóza   MeSH
• senzitivita a specificita MeSH
• sepse krev   diagnóza   MeSH
• stupeň závažnosti nemoci MeSH
• virové nemoci krev   diagnóza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH

• MeSH
• dítě MeSH
• ELISA MeSH
• infekce dýchací soustavy etiologie   krev   mikrobiologie   MeSH
• infekce získané v komunitě etiologie   krev   mikrobiologie   MeSH
• lidé MeSH
• předškolní dítě MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• předškolní dítě MeSH
• Geografické názvy
• Gruzie MeSH

• MeSH
• bronchoalveolární lavážní tekutina mikrobiologie   MeSH
• imunologické testy statistika a číselné údaje   MeSH
• infekce získané v komunitě krev   mikrobiologie   moč   MeSH
• Legionella genetika   imunologie   izolace a purifikace   MeSH
• legionelóza krev   mikrobiologie   moč   MeSH
• lidé MeSH
• polymerázová řetězová reakce statistika a číselné údaje   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• srovnávací studie MeSH