The localisation and distribution of the serotoninergic nerve elements was studied for the first time in the flatworm Chimaericola leptogaster (Leuckart, 1830) using immunocytochemical methodology and confocal laser scanning microscopy. The musculature was investigated by histochemical staining of actin filaments; scanning electron microscopy was used to identify the sensory structures on the worm's surface. Uniciliated, bi-ciliated and multiciliated sensory endings have been described on the worm's surface. The morphological data demonstrate the presence of circular, longitudinal and diagonal muscles that comprise the musculature of C. leptogaster in the anterior, median and posterior body regions. Well-developed radial and circular muscle fibres were also observed surrounding the genital pore, two vaginae and in clumps of the haptor. The study revealed the presence of biogenic amine, serotonin, in the central and peripheral nervous systems of C. leptogaster: in the neurons and fibres of the cephalic ganglia and ventral nerve cord, in the innervation of reproductive system compartments. The localised sites of the serotoninergic elements point to important roles of serotonin in monogenean reproductive processes and, possibly, in the regulation of muscle function.
- MeSH
- imunohistochemie veterinární MeSH
- konfokální mikroskopie veterinární MeSH
- mikroskopie elektronová rastrovací veterinární MeSH
- nervový systém - fyziologické jevy * MeSH
- nervový systém cytologie ultrastruktura MeSH
- ryby parazitologie MeSH
- serotonin analýza MeSH
- svaly cytologie ultrastruktura MeSH
- Trematoda cytologie fyziologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- Myokiny ( hormony svalové tkáně),
- MeSH
- ateroskleróza * diagnóza etiologie komplikace MeSH
- beta-buňky * cytologie enzymologie patologie MeSH
- cévní endotel metabolismus patofyziologie patologie MeSH
- cholesterol klasifikace metabolismus škodlivé účinky MeSH
- diabetes mellitus * etiologie metabolismus terapie MeSH
- diabetické angiopatie etiologie komplikace MeSH
- dyslipidemie diagnóza etiologie komplikace MeSH
- hyperglykemie etiologie metabolismus patofyziologie MeSH
- inzulinová rezistence fyziologie genetika imunologie MeSH
- komplikace diabetu diagnóza etiologie terapie MeSH
- lidé MeSH
- metaanalýza jako téma MeSH
- mikro RNA genetika metabolismus škodlivé účinky MeSH
- oxidační stres fyziologie genetika imunologie MeSH
- statistika jako téma MeSH
- svaly cytologie enzymologie metabolismus MeSH
- Check Tag
- lidé MeSH
BACKGROUND: Hemojuvelin (HJV) is one of essential components for expression of hepcidin, a hormone which regulates iron transport. HJV is mainly expressed in muscle and liver, and processing of HJV in both tissues is similar. However, hepcidin is expressed in liver but not in muscle and the role of the muscle HJV is yet to be established. Our preliminary analyses of mouse tissue HJV showed that the apparent molecular masses of HJV peptides are different in liver (50 kDa monomer and 35 and 20 kDa heterodimer fragments) and in muscle (55 kDa monomer and a 34 kDa possible large fragment of heterodimer). One possible explanation is glycosylation which could lead to difference in molecular mass. RESULTS: We investigated glycosylation of HJV in both liver and muscle tissue from mice. PNGase F treatment revealed that the HJV large fragments of liver and muscle were digested to peptides with similar masses, 30 and 31 kDa, respectively, and the liver 20 kDa small fragment of heterodimer was digested to 16 kDa, while the 50 kDa liver and 55 kDa muscle monomers were reduced to 42 and 48 kDa, respectively. Endo H treatment produced distinct digestion profiles of the large fragment: a small fraction of the 35 kDa peptide was reduced to 33 kDa in liver, while the majority of the 34 kDa peptide was digested to 33 kDa and a very small fraction to 31 kDa in muscle. In addition, liver HJV was found to be neuraminidase-sensitive but its muscle counterpart was neuraminidase-resistant. CONCLUSIONS: Our results indicate that different oligosaccharides are attached to liver and muscle HJV peptides, which may contribute to different functions of HJV in the two tissues.
- MeSH
- extracelulární prostor metabolismus MeSH
- genový knockout MeSH
- glykopeptidasa metabolismus MeSH
- glykosylace MeSH
- játra cytologie metabolismus MeSH
- membránové proteiny nedostatek genetika izolace a purifikace metabolismus MeSH
- myši MeSH
- neuraminidasa metabolismus MeSH
- orgánová specificita MeSH
- svaly cytologie metabolismus MeSH
- transport proteinů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- imunoenzymatické techniky MeSH
- izoenzymy metabolismus MeSH
- krysa rodu rattus MeSH
- morfogeneze MeSH
- myosiny biosyntéza metabolismus MeSH
- neurony eferentní fyziologie MeSH
- svaly cytologie inervace metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
