Myxosporean infections can cause severe damage to commercially grown tilapia. Here, we report a novel myxosporean that was found in gills of Oreochromis aureus male × Oreochromis niloticus female, which is an important aquaculture tilapia hybrid in Israel. Three-month-old fish were found to have cysts located in gill muscle tissue, which were filled with both immature and mature spores. Affected fish displayed higher mortality rate. Spore dimensions (10.8 ± 0.7 μm length × 6.8 ± 0.6 μm width) and molecular characterization using 18S ribosomal DNA revealed that the unknown parasite belongs in the Myxobolus clade. Based on the infection site, spore morphology and molecular characterization, we describe this parasite as Myxobolus bejeranoi n. sp. (MF401455). Phylogenetic analysis showed that the new species is most closely related to two Myxobolus spp. from O. niloticus in Egypt and Ghana.
- MeSH
- fylogeneze MeSH
- Myxobolus klasifikace genetika izolace a purifikace MeSH
- nemoci ryb parazitologie MeSH
- parazitární nemoci u zvířat parazitologie MeSH
- ribozomální DNA MeSH
- RNA ribozomální 18S genetika MeSH
- spory fyziologie MeSH
- svaly parazitologie MeSH
- Tilapia parazitologie MeSH
- žábry parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Izrael MeSH
Muscular sarcosporidial infections by Sarcocystis lutrae (Apicomplexa: Sarcocystidae) from the otter (Lutra lutra) and badger (Meles meles) (Carnivora: Mustelidae) were found in the Czech Republic. As part of a diversity evaluation of Sarcocystis in wild carnivores during 2016-2017, samples of diaphragm, tongue and hind-limb muscles were collected from nine districts, examined by compression and characterized molecularly. Cyst walls were thin, with no visible protrusions, and histological sections of infected muscle tissue showed no host responses. Fourteen of 17 badgers (82% prevalence) and one otter (100% prevalence) were positive for sarcocysts. Sequence analyses at four loci (18S rRNA, 28S rRNA, ITS1 and cox1) confirmed the identity as S. lutrae. This is also the first report of a co-infection with muscular sarcocystosis and Trichinella in badger. The finding of Trichinella is important from the zoonotic point of view, since badgers are used for meat consumption. Similar and future monitoring of both parasitic taxa are needed.
- MeSH
- Carnivora parazitologie MeSH
- koinfekce parazitologie veterinární MeSH
- molekulární typizace MeSH
- polymerázová řetězová reakce MeSH
- RNA ribozomální MeSH
- Sarcocystis klasifikace genetika izolace a purifikace MeSH
- sarkocystóza komplikace parazitologie veterinární MeSH
- svaly parazitologie MeSH
- Trichinella klasifikace genetika izolace a purifikace MeSH
- trichinelóza komplikace parazitologie veterinární MeSH
- vydry parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014-2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
- MeSH
- larva fyziologie MeSH
- morčata MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- svaly parazitologie MeSH
- teplota MeSH
- Trichinella klasifikace fyziologie MeSH
- trichinelóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- morčata MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Argentina MeSH
Muscular sarcocystosis by Sarcocystis arctica was found for the first time in the Czech Republic, in different muscles of red fox (Vulpes vulpes). Cysts were slim, elongated, thread-like, whitish, 1-7mm long, and 206-270μm wide; bradyzoites were 7.9×2.7μm in unstained wet mounts and 9.2×2.9μm in cyst Giemsa-stained smears. The cyst wall was thin, with short villi-like protrusions, and no host response was observed in the histological sections. Examination of the distribution and intensity of sarcocysts in 17 different muscle groups revealed that the highest intensity was in the cranial tibial muscle (>15 cysts in compressoria), followed by the diaphragm, forearm, and other groups (with intensities of 3-15 cysts in compressoria). Sarcocysts were detected in 3 out of 86 foxes. Genetic characterization at 18S rRNA, 28S rRNA, ITS1 and cox1, consistently showed that the species was identical with S. arctica. Interestingly, this protozoan was also detected as a co-infection in 3 foxes with the nematode Trichinella spp. for the first time.
- MeSH
- divoká zvířata parazitologie MeSH
- fylogeneze MeSH
- koinfekce epidemiologie parazitologie MeSH
- lišky parazitologie MeSH
- polymerázová řetězová reakce MeSH
- RNA ribozomální 18S genetika MeSH
- Sarcocystis cytologie genetika izolace a purifikace MeSH
- sarkocystóza diagnóza epidemiologie parazitologie veterinární MeSH
- sekvenční analýza DNA MeSH
- svaly parazitologie MeSH
- transmisní elektronová mikroskopie MeSH
- Trichinella izolace a purifikace MeSH
- trichinelóza epidemiologie parazitologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
A new nematode species, Philometroides acanthopagri sp. nov. (Philometridae), is described from gravid and subgravid specimens found in the musculature near pectoral fins and in nasal cavity of the yellowfin seabream Acanthopagrus latus (Houttuyn) (Sparidae, Perciformes) from marine waters off the coast of southern Iraq. Based on light and scanning electron microscopical examination, the new species differs from its congeners in a combination of morphological and biometrical features. It is the first species of Philometroides reported from a sparid fish and the first representative of this genus recorded from fishes in the Arabian Gulf. A key to Philometroides species parasitizing marine and brackish-water fishes is provided.
- MeSH
- biometrie MeSH
- Dracunculoidea anatomie a histologie izolace a purifikace MeSH
- mikroskopie MeSH
- mořská voda MeSH
- nematodózy parazitologie veterinární MeSH
- nemoci ryb parazitologie MeSH
- nosní dutina parazitologie MeSH
- Perciformes parazitologie MeSH
- ploutve zvířat parazitologie MeSH
- svaly parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Irák MeSH
Seven species of fishes, Catostomus commersonii (Lacépède), Etheostoma nigrum Rafinesque, Micropterus dolomieu Lacépède, Notemigonus crysoleucas (Mitchill), Notropis hudsonius (Clinton), Perca flavescens (Mitchill), and Percina caprodes (Rafinesque) from the St. Lawrence River, Quebec, Canada, were found infected with progenetic specimens of Neochasmus spp. in the orbits and/or the body musculature. Worms displayed varying degrees of maturation. Eggs occupied the entirety of the worm in late stages of development and persisted as distinct clusters in situ after worm death. Populations of parasites were studied monthly in E. nigrum from one site between May and October in order to follow parasite recruitment, development and maturation. Recruitment of parasites was observed in young-of-the-year fish primarily in July and continued through October. Worms matured rapidly, displaying egg production within a month. Later developmental stages, in which eggs occupied most of the worm, and clusters of eggs became abundant by September. Infections in overwintered fish collected in May consisted mainly of worms in early stages of egg production and of clusters of eggs. When hatched artificially, eggs from the clusters released viable miracidia, indicating that they survive beyond the lifespan of the adult worm. It is suggested that progenesis is a fixed characteristic of the life cycle of these species, that egg dispersal requires the death of the host and that it is facilitated by predation. All prior records of Neochasmus spp. are examined, leading us to conclude that the role of the putative definitive host (primarily basses) has been reduced to that of a dispersal agent. Current hypotheses concerning the evolution and maintenance of progenesis are considered, but it is concluded that they do not apply to this host-parasite system.
- MeSH
- infekce červy třídy Trematoda epidemiologie parazitologie veterinární MeSH
- nemoci ryb epidemiologie parazitologie MeSH
- oči parazitologie MeSH
- prevalence MeSH
- řeky MeSH
- roční období MeSH
- ryby MeSH
- stadia vývoje MeSH
- střeva parazitologie MeSH
- svaly parazitologie MeSH
- Trematoda růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- provincie Québec MeSH
- MeSH
- biopsie MeSH
- hospodářská zvířata parazitologie MeSH
- kočky MeSH
- lidé MeSH
- maso parazitologie MeSH
- myši parazitologie MeSH
- psi MeSH
- svaly parazitologie MeSH
- Trichinella imunologie izolace a purifikace růst a vývoj MeSH
- trichinelóza MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- lidé MeSH
- myši parazitologie MeSH
- psi MeSH
- zvířata MeSH
