AIM: To evaluate the frequency of the loss of the Adenomatous Polyposis Coli (APC) protein and to compare the APC status with the characteristics of colorectal adenomas. METHODS: Immunohistochemical analysis of the APC protein was performed on 118 adenomas and the results were compared with parameters of malignant potential, location of adenomas, macroscopic appearance and age of the patients. RESULTS: A complete loss of the APC protein was found in 28 (24%) adenomas, while 90 (76%) were APC positive. The mean size of adenomas was 13.5 +/- 14.2 mm (95% CI 10.5-16.5) in APC-positive, and 13.8 +/- 15.5 mm (95% CI 7.8-19.8) in APC-negative adenomas (P = 0.364). Statistical analysis revealed no difference between APC-positive and negative adenomas as to the histological type (P = 0.327) and grade of dysplasia (P = 0.494). We found that even advanced adenomas did not differ in their APC status from the non-advanced tumors (P = 0.414). Finally, no difference was found when the location (P = 0.157), macroscopic appearance (P = 0.571) and age of patients (P = 0.438) were analysed and compared between both APC positive and negative adenomas. CONCLUSION: Most adenomas expressed full-length APC protein, suggesting that protein expression is not a reliable marker for assessment of APC gene mutation. Complete loss of APC protein did not influence morphology, location, or appearance of adenomas, nor was it affected by the patient's age.

• MeSH
• adenom genetika   chemie   patologie   MeSH
• dospělí MeSH
• fenotyp MeSH
• financování organizované MeSH
• imunohistochemie MeSH
• kolorektální nádory genetika   chemie   patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mutace MeSH
• mutační analýza DNA MeSH
• nádorové biomarkery analýza   MeSH
• protein familiární adenomatózní polypózy analýza   genetika   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• stanovení celkové genové exprese MeSH
• stupeň závažnosti nemoci MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH

• MeSH
• familiární adenomatózní polypóza diagnóza   genetika   MeSH
• genetická predispozice k nemoci epidemiologie   MeSH
• kolorektální nádory diagnóza   klasifikace   terapie   MeSH
• lidé MeSH
• nádory anu diagnóza   epidemiologie   genetika   MeSH
• plošný screening využití   MeSH
• protein familiární adenomatózní polypózy analýza   MeSH
• tlusté střevo patologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH