The cancerogen 1,2-dimethylhydrazine (DMH), widely used in the experimental animal model of carcinogenesis, affects various organs, but its effect on muscle fibers is unknown. To evaluate the effect of 15-week DMH treatment on the fiber size and myosin heavy chain (MyHC) isoforms, which substantially determine fiber types and their contractile characteristics, pure and hybrid fiber types were immunohistochemically determined according to the MyHC isoform expression in soleus, extensor digitorum longus, gastrocnemius medialis and lateralis muscles of DMH-treated and control male Wistar rats. Whereas the size of fibers was mostly unaffected, the MyHC isoform expression was partially affected in both gastrocnemius samples, but not in the soleus and extensor digitorum longus of DMH-treated rats. The lower proportions of hybrid fiber types and especially that of type 1/2x in most gastrocnemius samples of DMH-treated rats resulted in a shift towards a single MyHC isoform expression, but the extent and pattern of the MyHC isoform shift varied across the different gastrocnemius samples. Such variable response to DMH treatment across muscles indicates that each muscle possesses its own adaptive range. These findings are essential for an accurate evaluation of skeletal muscle characteristics in DMH animal model.

• MeSH
• 1,2-Dimethylhydrazine toxicity   MeSH
• Carcinogens toxicity   MeSH
• Muscle Fibers, Skeletal drug effects   metabolism   pathology   MeSH
• Rats MeSH
• Rats, Wistar MeSH
• Muscle Fibers, Slow-Twitch drug effects   metabolism   pathology   MeSH
• Muscle Fibers, Fast-Twitch drug effects   metabolism   pathology   MeSH
• Myosin Heavy Chains biosynthesis   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

• MeSH
• 1,2-Dimethylhydrazine ultrasonography   MeSH
• Neck Pain etiology   ultrasonography   MeSH
• Humans MeSH
• Lymphadenitis ultrasonography   MeSH
• Lymph Nodes ultrasonography   MeSH
• Ultrasonography methods   MeSH
• Check Tag
• Humans MeSH

• MeSH
• Cholesterol analysis   blood   MeSH
• Diet MeSH
• Dimethylhydrazines adverse effects   MeSH
• Hypercholesterolemia MeSH
• Carcinogens MeSH
• Rats MeSH
• Disease Models, Animal MeSH
• Colonic Neoplasms pathology   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Animals MeSH

• MeSH
• Dimethylhydrazines adverse effects   MeSH
• Glucans MeSH
• Rats MeSH
• Disease Models, Animal diet therapy   pathology   MeSH
• Precancerous Conditions diet therapy   chemically induced   pathology   MeSH
• Intestine, Large pathology   MeSH
• Free Radicals MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Animals MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Cough etiology   MeSH
• Citric Acid administration & dosage   MeSH
• Guinea Pigs MeSH
• Ovalbumin administration & dosage   MeSH
• Rhinitis MeSH
• Animals MeSH
• Check Tag
• Guinea Pigs MeSH
• Animals MeSH
• Publication type
• Comparative Study MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Fractures, Bone surgery   therapy   MeSH
• Publication type
• Review MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Electronic Data Processing MeSH
• Menu Planning MeSH
• Humans MeSH
• Computers MeSH
• Surveys and Questionnaires MeSH
• Feeding Behavior MeSH
• Check Tag
• Humans MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Adult MeSH
• Hyperlipoproteinemias diagnosis   etiology   therapy   MeSH
• Hypolipidemic Agents administration & dosage   therapeutic use   MeSH
• Middle Aged MeSH
• Humans MeSH
• Risk Factors MeSH
• Aged MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Female MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Diabetes Mellitus, Type 2 diagnosis   drug therapy   therapy   MeSH
• Humans MeSH
• Check Tag
• Humans MeSH

• MeSH
• 1,2-Dimethylhydrazine MeSH
• Process Assessment, Health Care MeSH
• Humans MeSH
• Check Tag
• Humans MeSH