Q112542291
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AIMS: Mild Traumatic Brain Injury (mTBI) is the most common type of craniocerebral injury. Proper management appears to be a key factor in preventing post-concussion syndrome. The aim of this prospective study was to evaluate the effect and safety of selected training protocol in patients after mTBI. METHODS: This was a prospective study that included 25 patients with mTBI and 25 matched healthy controls. Assessments were performed in two sessions and included a post-concussion symptoms questionnaire, battery of neurocognitive tests, and magnetic resonance with tractography. Participants were divided into two groups: a passive subgroup with no specific recommendations and an active subgroup with simple physical and cognitive training. RESULTS: The training program with slightly higher initial physical and cognitive loads was well tolerated and was harmless according to the noninferiority test. The tractography showed overall temporal posttraumatic changes in the brain. The predictive model was able to distinguish between patients and controls in the first (AUC=0.807) and second (AUC=0.652) sessions. In general, tractography had an overall predictive dominance of measures. CONCLUSION: The results from our study objectively point to the safety of our chosen training protocol, simultaneously with the signs of slight benefits in specific cognitive domains. The study also showed the capability of machine learning and predictive models in mTBI patient recognition.
- MeSH
- dospělí MeSH
- komoce mozku * MeSH
- lidé středního věku MeSH
- lidé MeSH
- magnetická rezonanční tomografie MeSH
- mladý dospělý MeSH
- neuropsychologické testy MeSH
- postkomoční syndrom * MeSH
- prospektivní studie MeSH
- studie případů a kontrol MeSH
- zobrazování difuzních tenzorů MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Objective: The study aimed to determine the correlation between noninvasively ultrasonographically obtained cervical vessel indices and invasively measured central venous pressure in critically ill pediatric patients. Design: Prospective observational study. Setting: Department of pediatric intensive care, university hospital. Material and methods: 77 patients aged 0 to under 19 years with inserted central venous catheter and central venous pressure monitoring requiring admission to the department of intensive care medicine were included in the study. Both spontaneously ventilating patients and patients on artificial pulmonary ventilation, hemodynamically stable patients, and children with circulatory support with low-dose vasoactive agents were included in the study. Each continuous variable (ultrasonographic indices, age, height, weight, central venous pressure) was summarized by descriptive statistics, the distribution of variables was examined, and the correlation was quantified by Spearman correlation. The analysis was performed in the entire data set as well as in individual patient subgroups. Results: In the group of all measurements, the collapsibility index of the internal jugular vein in B mode (-0.58, p < 0.001), the collapsibility index of the internal jugular vein in M mode (-0.45, p <0.001), and the ratio of the internal jugular vein in 30- and 0-degree position (0.43, p < 0.001) had the highest correlation with central venous pressure. In the group of spontaneously ventilated patients, the highest correlation with central venous pressure was also found for the collapsibility index of internal jugular vein in B mode (-0.51, p < 0.001), and in patients on artificial pulmonary ventilation for the collapsibility index of internal jugular vein in B mode (- 0.55, p < 0.001) and the distensibility index of internal jugular vein in B mode (-0.55, p < 0.001). The correlation of the ratio of the internal jugular vein and common carotid artery with central venous pressure was low in all measurements group (0.25, p < 0.05), low in the spontaneously ventilated group (0.3, p < 0.05) and none in the artificially ventilated group (0.04). Conclusion: The results of the present study suggest that ultrasonographically obtained cervical vessel indices may provide a non-invasive, easily repeatable, bedside alternative to central venous pressure examination in the estimation of intravascular filling in critically ill pediatric patients.
- MeSH
- arteria carotis communis diagnostické zobrazování patologie MeSH
- centrální žilní tlak * MeSH
- dítě MeSH
- katetrizace centrálních vén MeSH
- kojenec MeSH
- korelace dat MeSH
- krk * diagnostické zobrazování patologie MeSH
- lidé MeSH
- mladiství MeSH
- nemoci cév diagnostické zobrazování patologie MeSH
- neparametrická statistika MeSH
- prospektivní studie MeSH
- ukazatele zdravotního stavu MeSH
- ultrasonografie metody MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- Publikační typ
- klinická studie MeSH
- pozorovací studie MeSH
Introduction: Self-efficacy is considered one of the key components of breastfeeding success and thus is important to explore. However, this requires reliable measuring tools. Goal: The aim of the study was to evaluate the psychometric properties of the BSES-SF as a measure of confidence in breastfeeding among Slovak mothers, and to determine the self-efficacy of mothers in breastfeeding and its related factors. Methods: The research was designed as a quantitative cross-sectional study. The sample consisted of 678 women who were on the 3rd-4th day after delivery. A Slovak version of the 14-item Breastfeeding Self-Efficacy Scale - Short Form (BSES-SF) was used to assess breastfeeding self-efficacy. Results: Cronbach's alpha of the Slovak version of the BSES-SF was 0.812. CFA has shown that the four-factor model of the BSES-SF offered the best fit for Slovak data. Education (p < 0.001), previous breastfeeding experience (p = 0.015), and skin-to-skin contact (p < 0.001) were shown to be statistically significant factors related to breastfeeding self-efficacy of mothers. Conclusion: The Slovak version of the BSES-SF has shown good psychometric properties and it can be recommended to assess the breastfeeding self-efficacy of mothers in Slovakia. The proven factors related to self-efficacy show the need for individual help, especially for women with higher education, women with a previous negative experience with breastfeeding, and the importance of skin-to-skin contact support.
Bronchial asthma is the most common chronic respiratory disease of childhood. Cough is one of its defining symptoms. This study investigated the associations between selected inflammatory biomarkers and cough reflex sensitivity after capsaicin inhalation in children with mild and moderate well-controlled type 2 endotype asthma compared with non-asthmatic probands. Sensitivity to the cough reflex was measured by recording the cough response after capsaicin inhalation. The sandwich ELISA method was used to measure serum concentrations of the investigated potential inflammatory biomarkers (interleukin 13, interleukin 1beta, eosinophil-derived neurotoxin). The acquired data were statistically evaluated according to descriptive analyses for summarization and comparison between cough reflex sensitivity parameters and individual biomarker values in the observed and control groups modeled by a simple linear regression model. Statistical significance was defined as p<0.05. We showed a statistically significant association (p-value 0.03) between cough reflex sensitivity - C2 value (capsaicin concentration required for two cough responses) and interleukin 1beta serum concentrations in the asthma group compared with the control group of non-asthmatic children. Our results support the possibility of interleukin 1beta as a potential additive inflammatory biomarker used in clinical practice in children with asthma because of its correlation with the activity of the afferent nerve endings in the airways.
- MeSH
- biologické markery MeSH
- bronchiální astma * diagnóza komplikace MeSH
- dítě MeSH
- interleukin-1beta MeSH
- kapsaicin MeSH
- kašel * etiologie MeSH
- lidé MeSH
- reflex fyziologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Úvod: Angioplastika dialyzačnej artériovenóznej fistuly pomocou liekových balónikov (DEB) versus konvenčnej balónikovej angioplastiky (PTA). Cieľ: Cieľom retrospektívnej štúdie bolo zhodnotiť a porovnať primárnu priechodnosť angioplastiky pomocou paclitaxelom potiahnutého a štandardného balónika v liečbe stenózy zlyhávajúcej dialyzačnej fistuly. Metodika: Retrospektívne zaradení boli dospelí pacienti s dysfunkciou dialyzačnej fistuly v rokoch 2015 - 2018 s maturovanou natívnou fistulou so stenózou viac ako 50%. Podľa liečby boli rozdelení na skupiny - DEB a PTA. Exklúzne kritériá boli restenóza, stenóza v "swing point oblastiach", v oblasti centrálnych vén a s viacpočetné vzdialené stenózy. Primárne sme hodnotili priechodnosť angioplastiky definovanej ako funkčnosť dialýzy bez potreby endovaskulárnej alebo chirurgickej intervencie na príčinnej lézii v ročnom sledovaní. Sekundárne ciele boli determinované ako priechodnosť dialýzačného prístupu, ročná asistovaná priechodnosť, technický, klinický úspech procedúry, miera periprocedurálnych komplikácií a mortalita v jednotlivých skupinách. Výsledky: Z 58 pacientov bola primárna priechodnosť v 6. a 12. mesiaci hodnotená u 25 v skupine DEB a 25 v skupine PTA. Primárna priechodnosť a priechodnosť dialýzačného prístupu bola - DEB vs. PTA v 6. mesiaci: 96 % vs. 76% (p = 0,1) a 96% vs. 72% (p = 0,049) a v 12. mesiaci: 80% vs. 56% (p = 0,13) a 80% vs. 52% (p = 0,073). Asistovaná priechodnosť bola v 1 roku DEB vs. PTA: 96% vs. 76% (p = 0,1). Primárny technický úspech bol DEB vs. PTA: 70% vs. 74% (p > 0,9), sekundárny 100% vs. 94 % (p = 0,5), klinický úspech: 100% vs. 97% (p = 0,9), celková miera komplikácií: 15 % vs. 9,7 % (p = 0,7), okrem jednej všetky nezávazné. Celkový počet zákrokov na prístupe za 12 mesiacov bol v skupine DEB štatisticky významne nižší: 5 vs. 14 (p = 0,02). Záver: Pacienti liečení pomocou DEB potrebovali v priebehu roka po intervencii štatisticky menej zákrokov pre zlyhanie v porovnaní so skupinou PTA.
Aim: The aim of our retrospective study was to evaluate primary patency of angioplasty using drug eluting balloon (DEB) and conventional balloon (PTA) in dysfunctional dialysis fistula. Methods: Fifty eight adult patients treated for fistula dysfunction in period from 2015 to 2018 were enrolled based on following criteria - matured native fistula with stenosis above 50%. Patients with restenosis, swing point, cephalic arch and central vein stenosis and multiple distant stenoses were ruled out. Patients were divided into two groups according to received treatment - DEB and PTA. Primary patency of angioplasty was defined as the function of dialysis without the need for clinically driven endovascular or surgical intervention on culprit lesion during follow up. Secondary, dialysis access patency, 12-month assisted patency, technical, clinical success, complication rate and mortality among treated groups were evaluated. Results: Primary patency at 6th and 12th months follow up was evaluated in 25 patients in the DEB and 25 patients in the PTA group. Primary and access patency were in DEB vs. PTA: 96% vs. 76% (p = 0.1) and 96% vs. 72% (p = 0.049) at 6 months, 80% vs. 56% (p = 0.13), 80% vs. 52% (p = 0.073) at 12 months. Assisted patency was: 96% vs. 76%, p = 0.1. Primary technical success was in DEB vs. PTA: 70% vs. 74% (p = 0.9), secondary 100% vs. 94% (p = 0.5), clinical success: 100% vs. 97% (p > 0.9), overall rate of complications 15% vs. 9.7% (p = 0.7), all but one mild. Number of interventions in access in 12 months was significantly lower in DEB group with 5 interventions compared to PTA group with 14 interventions (p = 0.02). Conclusion: Patients treated with DEB needed significantly less interventions on access during 12-month follow-up.
INTRODUCTION: Loss of consciousness (LOC) is used as a diagnostic feature of mild traumatic brain injury (MTBI). However, only 10% of concussions result in LOC. There are only a limited number of in-vivo studies dealing with unconsciousness and structural and functional integrity of the brainstem in patients with MTBI. The aim of our pilot study was to assess the sensitivity of proton magnetic resonance spectroscopy (1H-MRS) to detect metabolic changes in the brainstem in patients after MTBI with unconscioussness. METHODS: Twenty-four patients (12 with LOC, and 12 without LOC) within 3 days of MTBI and 19 healthy controls were examined. All subjects underwent single-voxel 1H-MRS examination of the upper brainstem. Spectra were evaluated using LCModel software. Ratios of total N-acetylaspartate (tNAA), total choline-containing compounds (tCho) and glutamate plus glutamine (Glx) to total creatine (tCre) were used for calculations. RESULTS: We found a significant decrease in tNAA/tCre and tCho/tCre ratios in the patient group with LOC when compared with the control group of healthy volunteers (P=0.002 and P=0.041, respectively), and a significant decrease in the tNAA/tCre ratio in the LOC group when compared with patients without LOC (P=0.04). Other metabolite ratios in the brainstem did not show any significant group differences. CONCLUSION: Our findings indicate that decrease of tNAA/tCre ratio in the upper brainstem using single-voxel 1H-MRS may provide a potential biomarker for MTBI associated with LOC.
Cieľ štúdie: Cieľom štúdie bolo identifikovať rizikové matky a skúmať súvislosti vybraných premenných (vek, vzdelanie, parita, podpora SSC, spokojnosť s podporou partnera, ochorenie dieťaťa, riziko depresie, finančné zabezpečenie rodiny) s popôrodnou vzťahovou väzbou. Metódy: Bol zvolený dizajn prierezovej štúdie. Výskumu sa zúčastnilo 204 žien (priemerný vek 30,9 ± 4,8 rokov), ktoré boli 6 týždňov po pôrode. Bol použitý dotazník popôrodnej vzťahovej väzby (Postpartum Bonding Questionnaire; PBQ) a škála popôrodnej depresie (Edinburgh Postnatal Depression Scale, EPDS). Dotazník bol doplnený kategorizačnými premennými, ktoré sa analyzovali vo vzťahu k štyrom faktorom PBQ, ktoré skúmali kvalitu vzťahovej väzby (F1), odmietnutie a patologický hnev (F2), úzkosť matky z dieťaťa (F3) a počiatočné zneužívanie dieťaťa (F4). Na analýzu údajov bola použitá deskriptívna štatistika, Wilcoxonov dvojvýberový test, Kruskalov-Wallisov test a Fisherov test. Výsledky: Pri skúmaní vzťahovej väzby dotazníkom PBQ sme identifikovali v F1 8,82 % rizikových matiek, v F2 0, 49 %, v F3 1,96 % a v F4 0,49 % rizikových matiek. Pomocou škály EPDS bolo riziko depresie identifikované u 22,72 % žien. Vo vzťahu k F1 sa preukázali štatisticky významné premenné vzdelanie (p = 0,001), spokojnosť s podporou partnera (p = 0,016) a riziko depresie (p = 0,001), k F2 riziko depresie (p = 0,001) a finančné zabezpečenie rodiny (p = 0,026), k F3 vzdelanie (p = 0,022), parita (p = 0,037), podpora SSC (p = 0,001), spokojnosť s podporou partnera (p = 0,050), riziko depresie (p = 0,001) a finančné zabezpečenie rodiny (p = 0,002) a k F4 podpora SSC (p = 0,047), riziko depresie (p = 0,002) a finančné zabezpečenie rodiny (p = 0,028). Závery: Identifikovanie rizikových matiek v súvislosti s bezpečnou vzťahovou väzbou s dôrazom na determinovanie jej súvisiacich premenných môže byť významné z hľadiska prevencie zlého zaobchádzania s deťmi, ktoré je potrebné riešiť včasnou intervenciou v rámci multidisciplinárnej spolupráce.
Objective: The objective of the study was to identify the risk mother and examine the relationship between selected variables (age, education, parity, skin-to-skin contact (SSC) support, partner support satisfaction, a child with an illness, risk of depression and family financial security) with postpartum mother-infant bonding. Methods: The research was designed as a cross-sectional study. The study sample consisted of 204 women (mean age 30.9±4.8 years) who were 6 weeks after childbirth. The Postpartum Bonding Questionnaire (PBQ) and Edinburgh Postnatal Depression Scale (EPDS) were used. The questionnaire was supplemented by research variables, which were analyzed in relation to four factors of PBQ, which examined quality of mother-infant bond (F1); rejection and pathological anger (F2); infant-focused anxiety (F3); incipient abuse of infant (F4). The prenatálnereceived data were analysed using descriptive statistics, the Wilcoxon two-sample test, the Kruskal-Wallis test and the Fisher test. Results: When examining mother-infant bonding with PBQ, we identified 8.82% of at-risk mothers in the F1; 0.49% in the F2, 1.96% in the F3 and in the F4 0.49% risk mothers were identified. Using the EPDS scale the risk of depression was identified 22.72% of the women. The following statistically significant variables shown in relation to the F1 education (p=0.001), partner support satisfaction (p=0.016) and risk of depression (p=0.001), to the F2 risk of depression (p=0.001) and family financial security (p=0.026), to the F3 education (p=0.022), parity (p=0.037), SSC support (p=0.001), partner support satisfaction (p=0.050), risk of depression (p=0.001) and family financial security (p=0.002) and to the F4 SSC support (p=0.047), risk of depression (p=0.002) and family financial security (p=0.028). Conclusion: Identification of risk mothers in relation to a secure attachment with an emphasis on determining its related variables, can be important in preventing maltreatment, which needs to be addressed through early intervention in multidisciplinary cooperation.
- Klíčová slova
- rizikové matky,
- MeSH
- dítě MeSH
- lidé MeSH
- novorozenec MeSH
- poporodní období MeSH
- průzkumy a dotazníky MeSH
- vztahy mezi matkou a dítětem * MeSH
- zneužívané dítě prevence a kontrola MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
Autism spectrum disorder (ASD) represents a serious neurodevelopmental disorder associated with autonomic nervous system dysregulation. The aim was to study complex cardiovascular autonomic regulation using heart rate variability (HRV) and systolic blood pressure variability (SBPV) linear/non-linear analysis at rest and during orthostasis, and to assess plasma levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) in autistic children. Twenty-five ASD boys and 25 age and gender-matched children at the age 7-15 years were examined. After venous blood taking, continuous ECG and blood pressure biosignals were recorded at rest and during orthostasis. Evaluated parameters: RR intervals, high- and low-frequency band of HRV spectral analysis (HF-HRV, LF-HRV), symbolic dynamics parameters 0V%, 1V%, 2LV%, 2UV%, low- and high-frequency band of SBPV (LF-SBPV, HF-SBPV), systolic, diastolic, mean blood pressure, EGF, VEGF plasma levels. RR intervals were significantly shortened and the HF-HRV, LF-SBPV, HF-SBPV parameters were significantly lower at rest, the HF-HRV and LF-SBPV remained lower during orthostasis in autistic children compared to controls (p<0.05). EGF plasma levels were significantly lower in ASD compared to controls (p=0.046). No significant differences were found in remaining parameters. Our study revealed tachycardia, cardiovagal underactivity, and blunted sympathetic vasomotor regulation at rest and during orthostasis in autistic children. Additionally, complex heart rate dynamics are similar in autistic children than controls. Furthermore, EGF was reduced in autistic children without significant correlations with any autonomic parameters. We suggest that the abnormal complex cardiovascular reflex control could contribute to understanding the pathway linking autonomic features and autism.
- MeSH
- autonomní nervový systém patofyziologie MeSH
- baroreflex MeSH
- biologické markery krev MeSH
- dítě MeSH
- epidermální růstový faktor krev MeSH
- krevní tlak * MeSH
- lidé MeSH
- mladiství MeSH
- poruchy autistického spektra krev diagnóza patofyziologie MeSH
- srdce inervace MeSH
- srdeční frekvence * MeSH
- vaskulární endoteliální růstový faktor A krev MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Pankreatický duktální adenokarcinom (PDAC) představuje jeden z nejagresivnějších typů lidských malignit. V současnosti je toto zhoubné onemocnění čtvrtou nejčastější příčinou úmrtí na rakovinu. Pětileté přežití pacientů s duktálním adenokarcinomem je méně než 8 %. Nové in vitro a in vivo modely jsou proto nutně potřebné pro vývoj nových terapií. S vlastní technologií pro derivaci nových, unikátních 3D nádorových buněčných linií izolovaných z lidských nádorů a ve spolupráci s Ústavem živočišné fyziologie a genetiky AV ČR v Liběchově představujeme plán vývoje velkého zvířecího modelu pro modelování lidského PDAC. S využitím tohoto modelu a nejmodernějších laboratorních technik provedeme profilovací analýzy (detekce a fenotypování cirkulujících rakovinných buněk, izolace a sekvenování cirkulující DNA, metabolomické profilování a analýzu onkoproteinů, a detekce cytokinů pomocí multiplexních protilátkových čipů za účelem nalezení biomarkerů nádoru pankreatu. Nově vyvinutý model zároveň poskytneme pro potřeby výzkumu spolupracujícím vědeckým pracovištím a farmaceutickým společnostem.
Pancreatic ductal adenocarcinoma represents one of the most aggressive type of human malignancy. Currently, this malignancy is the fourth most frequent cause of dead. 5-year survival of patients with ductal adenocarcinoma is less than 8 %. New in vitro and in vivo models are therefore desperately needed for new therapy development. With our own technology for derivation of new, unique 3D cancer cell lines from human tumors and in collaboration with Institute of Animal Physiology and Genetics, AS CR, in Libechov, we present here the plan for the development of large xenograft animal model (pig) bearing human pancreatic tumor. With the use of this model and state -of -the -art lab.techniques, we will perform multiple profiling analyses (circulating cancer cell detection and phenotyping, circulating DNA isolation and sequencing, metabolomic profiling and cancer -related proteins and cytokines detection with multiplex antibody array chips in order to find the hallmarks of pancreatic tumor.
