Věkové aspekty vzniku hypertense: úloha genetických faktorů a vliv prostředí v časných fázích ontogeneze. XXX XXX XXX

• MeSH
• hypertenze genetika   etiologie   MeSH
• věkové faktory MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• kardiologie
• angiologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

The contribution of chloride to the haemodynamic changes of salt-dependent deoxycorticosterone (DOC) hypertension was studied in young Wistar rats subjected to dietary loading with sodium chloride (NaCl) or sodium bicarbonate (NaHCO3). Mean arterial pressure (MAP), cardiac output, systemic resistance (TPR) and arterial rigidity (estimated from pulse pressure/stroke volume ratio, PP/SV) were determined in conscious chronically cannulated rats. DOC-induced increase of MAP and TPR appeared earlier in NaCl-loaded than in NaHCO3-loaded rats. After 4-6 weeks of hypertensive treatment MAP, TPR and PP/SV ratio were higher in DOC-treated rats fed NaCl diet than in those fed NaHCO3 diet. In contrast, after a long-term hypertensive regimen (lasting for 7-9 weeks) there was no significant difference in either MAP or TPR between rats loaded with NaCl or NaHCO3. On the other hand, DOC hypertension induced by a long-term feeding of NaHCO3 diet was not associated with an increase of arterial rigidity which was characteristic for DOC-NaCl hypertensive rats. Thus, a sufficiently long selective dietary sodium loading is capable to increase the systemic resistance but not to alter the arterial rigidity. This was also confirmed by a comparison of blood pressure-matched DOC hypertensive rats fed NaCl or NaHCO3 diets. These animals did not differ in the degree of systemic resistance elevation but the arterial rigidity was increased only in NaCl-loaded rats.

• MeSH
• cévní rezistence účinky léků   MeSH
• deoxykortikosteron * farmakologie   MeSH
• dieta MeSH
• hemodynamika fyziologie   účinky léků   MeSH
• hydrogenuhličitan sodný * farmakologie   MeSH
• hypertenze chemicky indukované   patofyziologie   MeSH
• krevní tlak účinky léků   MeSH
• krysa rodu rattus MeSH
• kuchyňská sůl * farmakologie   MeSH
• minutový srdeční výdej účinky léků   MeSH
• potkani Wistar MeSH
• pulz MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• chlorid sodný toxicita   MeSH
• difenylhexatrien analogy a deriváty   MeSH
• erytrocytární membrána fyziologie   MeSH
• fluidita membrány MeSH
• hypertenze etiologie   genetika   krev   MeSH
• krevní tlak genetika   MeSH
• krysa rodu rattus MeSH
• modely nemocí na zvířatech MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• MeSH
• angiotensin I metabolismus   MeSH
• antagonisté receptorů pro angiotenzin MeSH
• gama-globuliny imunologie   MeSH
• krysa rodu rattus MeSH
• molekulární sekvence - údaje MeSH
• peptidové fragmenty chemická syntéza   imunologie   MeSH
• receptory angiotensinu imunologie   MeSH
• renovaskulární hypertenze patofyziologie   prevence a kontrola   MeSH
• sekvence aminokyselin MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH

Several animal models of genetic hypertension have been developed but not all of them possess a closely related control strain. Therefore, a new model based on Wistar rats is described in which both hypertensive and normotensive lines were bred from a single parental pair. Several basic data on the two lines (called the Prague Hypertensive Rat, PHR, and the Prague Normotensive Rat, PNR) are given. PNR had a longer survival compared with PHR. At the age of 7 weeks, systolic blood pressure was 161 +/- 14 mmHg in PHR males and 109 +/- 9 mmHg in PNR males. Its further increase with age was very slow in PNR but very steep in PHR. Typical left ventricular cardiac hypertrophy developed in PHR in which cardiac output was not significantly different from that of PNR but total peripheral resistance was higher. Kidney weight was also greater in PHR than in PNR. There was no difference in basic renal functions except of proteinuria which was higher in PHR than in PNR. No differences were observed in extracellular and interstitial fluid volumes whereas plasma and blood volumes were slightly but significantly greater in PHR than in PNR suggesting a shift of extracellular fluid towards the intravascular compartment. This hypertensive model the parameters of which resemble to those of human essential hypertension should be especially suitable for cross-transplantation studies.

• MeSH
• hypertenze etiologie   genetika   patofyziologie   MeSH
• inbrední kmeny potkanů MeSH
• krevní tlak MeSH
• krysa rodu rattus MeSH
• ledviny patofyziologie   MeSH
• modely nemocí na zvířatech MeSH
• potkani inbrední SHR * fyziologie   genetika   MeSH
• tělesné tekutiny fyziologie   MeSH
• velikost orgánu MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• aldosteron krev   MeSH
• chlorid sodný analýza   farmakokinetika   farmakologie   MeSH
• ileum enzymologie   MeSH
• jejunum enzymologie   MeSH
• kolon enzymologie   MeSH
• krysa rodu rattus MeSH
• sodík analýza   farmakokinetika   farmakologie   MeSH
• sodíko-draslíková ATPasa analýza   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• MeSH
• extracelulární prostor patologie   MeSH
• krysa rodu rattus MeSH
• modely nemocí na zvířatech MeSH
• objem plazmy MeSH
• renální hypertenze etiologie   MeSH
• renin-angiotensin systém MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• MeSH
• chlorid sodný aplikace a dávkování   škodlivé účinky   MeSH
• inbrední kmeny potkanů MeSH
• krevní tlak účinky záření   MeSH
• krysa rodu rattus MeSH
• presoreceptory MeSH
• reflex fyziologie   MeSH
• srdeční frekvence fyziologie   MeSH
• věkové faktory MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH

Red blood cell Na+ content as well as ouabain-resistant Na+ and Rb+ (K+) transport (susceptible or resistant to inhibition by loop diuretics) were determined in spontaneously hypertensive rats (SHR) and normotensive Brown Norway (BN) rats the erythrocytes of which were incubated in either saline or Mg(2+)-sucrose medium. Elevated ouabain-resistant Na+ net uptake contrasted with slightly decreased red blood cell Na+ content in SHR compared with BN rats. Acceleration of furosemide- and bumetanide-sensitive Na+ fluxes contributed to enhanced ouabain-resistant Na+ influx into SHR erythrocytes in saline medium, whereas higher furosemide- or bumetanide-resistant Na+ efflux caused greater ouabain-resistant Na+ efflux in Mg(2+)-sucrose medium. Furosemide- and bumetanide-resistant Rb+ leaks were augmented in SHR erythrocytes. The association of the disclosed ion transport alterations with blood pressure was examined in 20 recombinant inbred strains derived from F2 SHR x BN hybrids. Ouabain-resistant Na+ uptake as well as furosemide- and bumetanide-resistant Na+ inward leaks (but not red blood cell Na+ content or furosemide- and bumetanide-sensitive Na+ net uptake) cosegregated with systolic and pulse pressures but not diastolic pressure of the recombinant inbred strains. In contrast, neither ouabain-resistant Na+ efflux nor any component of ouabain-resistant Rb+ uptake correlated positively with blood pressure of the recombinant inbred strains. Increased ouabain-resistant Na+ influx was compensated for by accelerated ouabain-sensitive Na+ extrusion because red blood cell Na+ content was not elevated in the hypertensive strains. Thus, high cell Na+ turnover rates might be related to genetic hypertension if an altered Na+ inward leak would be less effectively compensated for in tissues involved in cardiovascular regulation.

• MeSH
• analýza rozptylu MeSH
• biologický transport MeSH
• bumetanid farmakologie   MeSH
• draslík farmakokinetika   MeSH
• erytrocyty * metabolismus   MeSH
• furosemid farmakologie   MeSH
• genetické markery MeSH
• hybridizace genetická MeSH
• hypertenze * patofyziologie   MeSH
• krevní tlak genetika   účinky léků   MeSH
• krysa rodu rattus MeSH
• ouabain farmakologie   MeSH
• potkani inbrední BN MeSH
• potkani inbrední SHR MeSH
• rubidium farmakokinetika   MeSH
• sodík * farmakokinetika   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• bumetanid farmakologie   MeSH
• erytrocyty metabolismus   účinky léků   MeSH
• furosemid farmakologie   MeSH
• hypertenze chemicky indukované   krev   patofyziologie   MeSH
• krysa rodu rattus MeSH
• ouabain farmakologie   MeSH
• sodík dietní MeSH
• sodík krev   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH