In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.

• MeSH
• biokompatibilní materiály farmakologie   MeSH
• dextrany chemie   MeSH
• emulze chemie   MeSH
• jehly MeSH
• kolagen typ II metabolismus   MeSH
• křenová peroxidasa metabolismus   MeSH
• mezenchymální kmenové buňky cytologie   účinky léků   metabolismus   MeSH
• mezibuněčné signální peptidy a proteiny farmakologie   MeSH
• miniaturní prasata MeSH
• nanovlákna chemie   ultrastruktura   MeSH
• poloxamer chemie   MeSH
• polyestery chemie   MeSH
• prasata MeSH
• proteiny aplikace a dávkování   MeSH
• rhodaminy chemie   MeSH
• tkáňové inženýrství metody   MeSH
• tkáňové podpůrné struktury chemie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Herein, we describe a simple spinneret setup for needleless coaxial electrospinning that exceeds the limited production capacity of current approaches. The proposed weir spinneret enables coaxial electrospinning from free liquid surface. This approach leads to the formation of coaxial nanofibers with higher and uniform shell/core ratio, which results in the possibility of better tuning of the degradation rate. The throughput and quality increase favor the broader application of coaxial nanofibers from weir spinnerets as systems for controlled drug delivery in regenerative medicine and tissue engineering.

• MeSH
• farmaceutická technologie metody   MeSH
• lékové transportní systémy * MeSH
• léky s prodlouženým účinkem MeSH
• lidé MeSH
• nanovlákna * MeSH
• regenerativní lékařství metody   MeSH
• tkáňové inženýrství metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

V rámci projektu bude vytvořen systém založený na dutých („core/shell”) nanovláknech obohacených o liposomy a PRP jako nového systému pro řízené dodávání léčiv především pro regeneraci osteochondrálních defektů. Tento postup, který je založen na stávajících dosažených výsledcích a patentech týmu řešitele Doc. RNDr. Evžena Amlera, CSc., povede k funkcionalizaci nanovláken a novému systému s imobilizovanými liposomy. Liposomy budou plněny růstovými a diferenciačními faktory. Počítáme s testováním nového systému jako inteligentního buněčného nosiče s prodlouženou retencí bioaktivníck látek při osídlování nosičů v podmínkách in vitro.; The objective of this study was to establish the system based on core/shell nanofibers enriched with liposomes as a novel drug delivery system for regeneration of osteochondral defects. Liposomes can be fulfilled with growth and differentiation factors. This system would lead to functionalization of nanofibers and presentation of nanofibers system with immobilized and anchored liposomes. We are counting on development of new system of intelligent cell scaffold with prolonged retention of bioactive bodies while being anriched by cells in conditions in vitro.. This approach is based on current research results and patents of Doc. RNDr. Evzen Amler, CSc. and his team. The project will be executed from 2011 to 2015 and the key outcome is prototype of new target delivery system. The benefits form this application will be not only for patients themselves, but also will save money for public insurence system.

• MeSH
• artritida terapie   MeSH
• lékové transportní systémy MeSH
• liposomy MeSH
• nanovlákna MeSH
• terapie měkkých tkání MeSH
• tkáňové inženýrství MeSH

• Konspekt
• Biotechnologie. Genetické inženýrství
• NLK Obory
• revmatologie
• traumatologie
• biomedicínské inženýrství
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

AIM: Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers. MATERIALS & METHODS: A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined. RESULTs: Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased. CONCLUSION: The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.

• MeSH
• chondrocyty cytologie   účinky léků   MeSH
• cytoplazmatická granula chemie   MeSH
• konfokální mikroskopie MeSH
• kultivované buňky MeSH
• lékové transportní systémy metody   MeSH
• lidé MeSH
• mezenchymální kmenové buňky cytologie   účinky léků   MeSH
• nanovlákna chemie   MeSH
• tkáňové podpůrné struktury chemie   MeSH
• transformující růstový faktor beta1 MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The broader application of liposomes in regenerative medicine is hampered by their short half-life and inefficient retention at the site of application. These disadvantages could be significantly reduced by their combination with nanofibers. We produced 2 different nanofiber-liposome systems in the present study, that is, liposomes blended within nanofibers and core/shell nanofibers with embedded liposomes. Herein, we demonstrate that blend electrospinning does not conserve intact liposomes. In contrast, coaxial electrospinning enables the incorporation of liposomes into nanofibers. We report polyvinyl alcohol-core/poly-ε-caprolactone-shell nanofibers with embedded liposomes and show that they preserve the enzymatic activity of encapsulated horseradish peroxidase. The potential of this system was also demonstrated by the enhancement of mesenchymal stem cell proliferation. In conclusion, intact liposomes incorporated into nanofibers by coaxial electrospinning are very promising as a drug delivery system.

• MeSH
• křenová peroxidasa metabolismus   MeSH
• lékové transportní systémy MeSH
• lidé MeSH
• liposomy chemie   MeSH
• mezenchymální kmenové buňky cytologie   metabolismus   MeSH
• nanovlákna chemie   MeSH
• nosiče léků chemie   metabolismus   MeSH
• povrchové vlastnosti MeSH
• proliferace buněk MeSH
• velikost částic MeSH
• viabilita buněk MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH