Martiniaková, M* Dotaz Zobrazit nápovědu
- MeSH
- antropologie metody MeSH
- femur anatomie a histologie virologie MeSH
- Haversův systém anatomie a histologie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- antropologie fyzická metody MeSH
- diafýzy anatomie a histologie virologie MeSH
- femur anatomie a histologie virologie MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- experimenty na zvířatech MeSH
- exprese genu genetika MeSH
- finanční podpora výzkumu jako téma MeSH
- kostní denzita genetika MeSH
- lidé MeSH
- lokus kvantitativního znaku genetika MeSH
- molekulární biologie metody trendy MeSH
- myši genetika MeSH
- osteoporóza etiologie genetika MeSH
- polymerázová řetězová reakce metody trendy využití MeSH
- rizikové faktory MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů metody trendy využití MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši genetika MeSH
- zvířata MeSH
Differences in microscopic structure of the femur between 1-month-old transgenic rabbits carrying the hFVIII gene and non-transgenic rabbits were investigated. Bone microstructure was evaluated from the point of view of qualitative and quantitative histological characteristics. We identified fibrolamellar bone tissue only in the transgenic animals. Measured values for area, perimeter of the Haversian canals and minimum diameter of the primary osteons' vascular canals were higher in 1-month-old transgenic individuals (P < 0.05; P < 0.001). We also observed lower concentrations of Ca, P, K, solids, and total mineral content in femora of transgenic rabbits. A statistically significant difference was observed for the concentration of Ca (P < 0.05). Our results indicate evident changes in both qualitative and quantitative histological characteristics of the femur, which result especially in better blood supply and slightly reduced mineralization process in 1-month-old transgenic rabbits.
- MeSH
- faktor VIII genetika MeSH
- femur cytologie MeSH
- fyziologická kalcifikace MeSH
- geneticky modifikovaná zvířata MeSH
- kostní denzita MeSH
- králíci MeSH
- lidé MeSH
- mléčné bílkoviny genetika MeSH
- novorozená zvířata MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
Acrylamide (AA) is a highly reactive organic compound capable of polymerization to form polyacrylamide, which is commonly used throughout a variety of industries. Given its toxic effect on humans and animals, the last 20 years have seen an increased interest in research devoted to the AA. One of the main sources of AA is food. AA appears in heated food following the reaction between amino acids and reduced sugars. Large concentrations of AA can be found in popular staples such as coffee, bread or potato products. An average daily consumption of AA is between 0.3-2.0 microg/kg b.w. Inhalation of acrylamide is related with occupational exposure. AA delivered with food is metabolized in the liver by cytochrome P450. AA biotransformation and elimination result in formation of toxic glycidamide (GA). Both, AA and GA can be involved in the coupling reaction with the reduced glutathione (GSH) forming glutathione conjugates which are excreted with urine. Biotransformation of AA leads to the disturbance in the redox balance. Numerous research proved that AA and GA have significant influence on physiological functions including signal propagation in peripheral nerves, enzymatic and hormonal regulation, functions of muscles, reproduction etc. In addition AA and GA show neurotoxic, genotoxic and cancerogenic properties. In 1994, International Agency for Research on Cancer (IARC) classified acrylamide as a potentially carcinogenic substance to human.
- MeSH
- akrylamid farmakokinetika otrava MeSH
- analýza potravin metody MeSH
- aplikace orální MeSH
- kontaminace potravin analýza prevence a kontrola MeSH
- lidé MeSH
- nemoci ledvin chemicky indukované patofyziologie MeSH
- poškození DNA * MeSH
- rozmnožování účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Some vegetable oils are currently being promoted as a safe alternative to commercial sunscreens. The true UVB photoprotective efficacy of 14 virgin vegetable oils and the suitability of the dilution method for determining their SPF value were evaluated. Oils and standard sunscreens were investigated in vitro by the Mansur's method in Slovakia and in vivo by the ISO method in the Czech Republic. SPF values in vitro (0.1; 0.0; 0.4; 0.2 and 0.2) and in vivo (2.5; 1.2; 2.6; 2.6; and 2.8) of the five most promoted oils (from carrot seed, coconut, raspberry seed, rosehip seed, and wheat germ) were significantly lower than the values reported in the controversial studies. We have shown that the overestimated SPF values of these oils were determined by authors who did not strictly follow Mansur's original methodology. The other eight vegetable oils also provide no or negligible SPF values. Only the in vitro SPF value of 11.2 tamanu oil is worth mentioning, probably due to high proportion of calophyllolides. In vitro and in vivo SPF ratios from 1.14 to 0.94 obtained by two methods in two laboratories for six commercial sunscreen oils used as controls confirm the correctness of performing the Mansur's method in this study. However, this dilution method has proven to be fundamentally flawed in determining the SPF value of substances with such negligible photoprotection as most vegetable oils can provide. An SPF value of less than 1, which can be determined by this Mansur's method, is physiologically impossible and meaningless.
- MeSH
- benzimidazoly chemie MeSH
- dospělí MeSH
- kůže účinky záření MeSH
- lidé středního věku MeSH
- lidé MeSH
- ochranný sluneční faktor MeSH
- oleje rostlin chemie MeSH
- přípravky chránící proti slunci chemie MeSH
- Rubus chemie metabolismus MeSH
- semena rostlinná chemie metabolismus MeSH
- senioři MeSH
- ultrafialové záření * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Taurine, a sulphur - containing amino acid, has been termed a functional nutrient. Its synthetic form is a common ingredient in supplements and energy drinks. There is no information concerning taurine impact on bone microstructure after prolonged supplemental use. Also, differences in bone parameters of mice following taurine exposure are unknown. In this study, a detailed microstructure of compact and trabecular bone tissues of mice subchronically exposed to taurine was determined. Animals (n=12) were segregated into three groups: E1 group - mice received 20 mg/kg b.w. of taurine per day during 8 weeks; E2 group - mice were fed by taurine at a dose of 40 mg/kg b.w. for 8 weeks and a control (C) group. Decreased density of secondary osteons, increased sizes of primary osteon's vascular canals (P<0.05) were observed in taurine - treated animals. Cortical bone thickness, trabecular thickness were decreased (P<0.05) in E1 group, and relative volume of trabecular bone was lower (P<0.05) in E2 group as compared to C group. According to our results, prolonged taurine exposure at the doses used in this study can negatively affect both compact and trabecular bone tissues microstructure.
- MeSH
- femur účinky léků patologie fyziologie MeSH
- kortikální kost cytologie účinky léků fyziologie MeSH
- kostní denzita účinky léků fyziologie MeSH
- myši MeSH
- náhodné rozdělení MeSH
- rozvrh dávkování léků MeSH
- taurin aplikace a dávkování toxicita MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
