- MeSH
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics therapy MeSH
- Genetic Therapy methods trends MeSH
- Genome, Human genetics MeSH
- HIV Infections genetics therapy MeSH
- Disease Vectors MeSH
- Mosquito Vectors genetics MeSH
- Liposomes MeSH
- Drug Carriers MeSH
- RNA Interference physiology immunology MeSH
- Clustered Regularly Interspaced Short Palindromic Repeats * genetics MeSH
Naklíčená semena jsou potraviny bohaté na vitaminy, minerální látky, bílkoviny, enzymy a další pro tělo prospěšné látky. Z tohoto důvodu se v posledních letech zvýšil jak prodej, tak i domácí příprava naklíčených semen. Při procesu klíčení však nastávají ideální podmínky pro růst patogenních i nepatogenních mikroorganizmů a konzumace naklíčených semen může představovat zvýšené riziko alimentárních onemocnění. Výrobci i dozorové orgány proto dbají na preventivní opatření proti jejich kontaminaci a zároveň semena i již naklíčená semena před distribucí ke spotřebiteli testují na přítomnost patogenních mikroorganizmů. Legislativně jsou stanovena kritéria pro Listeria monocytogenes a Salmonella spp.
Sprouted seeds are rich in vitamins, minerals, proteins, enzymes and other beneficial substances for the body. For this reason, increasing sales and home production of these sprouts are recorded. However, the process of germination represents ideal conditions for the growth of pathogenic and non-pathogenic microorganisms. Therefore, any contamination with pathogenic microorganisms at the beginning or during the production poses a risk of foodborne infection. Manufacturers and supervisory authorities follow measures to prevent contamination and the seeds and sprouts are tested for the presence of pathogenic microorganisms. The production of sprouts has legislatively defined food safety criteria for Listeria monocytogenes and Salmonella spp.
- MeSH
- Edible Grain * MeSH
- Germination MeSH
- Humans MeSH
- Nutritive Value MeSH
- Food Microbiology standards MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Novel biomaterials based on hydrophilic polycaprolactone and polyurethane (Tecophilic®) nanofibers with an encapsulated 5,10,5,20-tetraphenylporphyrin photosensitizer were prepared by electrospinning. The doped nanofiber textiles efficiently photo-generate O(2)((1)Δ(g)), which oxidize external chemical and biological substrates/targets. Strong photo-virucidal effects toward non-enveloped polyomaviruses and enveloped baculoviruses were observed on the surface of these textiles. The photo-virucidal effect was confirmed by a decrease in virus infectivity. In contrast, no virucidal effect was detected in the absence of light and/or the encapsulated photosensitizer.
- MeSH
- Anthracenes chemistry MeSH
- Antiviral Agents pharmacology MeSH
- Baculoviridae drug effects MeSH
- Photosensitizing Agents pharmacology MeSH
- Virus Inactivation drug effects MeSH
- Capsid chemistry MeSH
- Mice MeSH
- Nanofibers chemistry ultrastructure MeSH
- Oxidation-Reduction MeSH
- Polyesters chemistry MeSH
- Polyomavirus drug effects MeSH
- Polyurethanes chemistry MeSH
- Porphyrins pharmacology MeSH
- Recombination, Genetic genetics MeSH
- Singlet Oxygen metabolism MeSH
- Textiles * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The aim of this study was to develop a suitable vaccine antigen against porcine circovirus 2 (PCV2), the causative agent of post-weaning multi-systemic wasting syndrome, which causes significant economic losses in swine breeding. Chimeric antigens containing PCV2b Cap protein sequences based on the mouse polyomavirus (MPyV) nanostructures were developed. First, universal vectors for baculovirus-directed production of chimeric MPyV VLPs or pentamers of the major capsid protein, VP1, were designed for their exploitation as vaccines against other pathogens. Various strategies were employed based on: A) exposure of selected immunogenic epitopes on the surface of MPyV VLPs by insertion into a surface loop of the VP1 protein, B) insertion of foreign protein molecules inside the VLPs, or C) fusion of a foreign protein or its part with the C-terminus of VP1 protein, to form giant pentamers of a chimeric protein. We evaluated these strategies by developing a recombinant vaccine against porcine circovirus 2. All candidate vaccines induced the production of antibodies against the capsid protein of porcine circovirus after immunization of mice. The candidate vaccine, Var C, based on fusion of mouse polyomavirus and porcine circovirus capsid proteins, could induce the production of antibodies with the highest PCV2 neutralizing capacity. Its ability to induce the production of neutralization antibodies was verified after immunization of pigs. The advantage of this vaccine, apart from its efficient production in insect cells and easy purification, is that it represents a DIVA (differentiating infected from vaccinated animals) vaccine, which also induces an immune response against the mouse polyoma VP1 protein and is thus able to distinguish between vaccinated and naturally infected animals.
- MeSH
- Circovirus * genetics immunology MeSH
- Mice MeSH
- Nanostructures * MeSH
- Polyomavirus * genetics immunology MeSH
- Swine MeSH
- Recombinant Fusion Proteins * genetics immunology MeSH
- Sf9 Cells MeSH
- Spodoptera MeSH
- Capsid Proteins * genetics immunology MeSH
- Viral Vaccines * genetics immunology pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
JC and BK polyomaviruses (JCV and BKV) infect humans and can cause severe illnesses in immunocompromised patients. Merkel cell polyomavirus (MCPyV) can be found in skin carcinomas. In this study, we assessed the occurrence of serum antibodies against MCPyV, BKV, and JCV polyomaviruses in a healthy population of the Czech Republic. Serum samples from 991 healthy individuals (age range: 6-64 years) were examined by enzyme-linked immunoassay (ELISA) using virus-like particles (VLPs) based on the major VP1 capsid proteins of these viruses. Overall, serum antibodies against MCPyV, JCV, and BKV were found in 63%, 57%, and 69%, respectively, of this population. For all three viruses, these rates were associated with age; the occurrence of antibodies against MCPyV and JCV was highest for those older than 59 years, while the occurrence of antibodies against BKV was highest in those aged 10-19 years and 20-29 years. This is the first large study to determine the seroprevalence rates for BKV, JCV, and MCPyV polyomaviruses in the general Czech Republic population.
- MeSH
- Cell Line MeSH
- Child MeSH
- Adult MeSH
- Tumor Virus Infections blood epidemiology virology MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Merkel cell polyomavirus immunology MeSH
- Adolescent MeSH
- Young Adult MeSH
- Mice, Inbred ICR MeSH
- Polyomavirus Infections blood epidemiology virology MeSH
- Child, Preschool MeSH
- Antibodies, Viral blood MeSH
- Seroepidemiologic Studies MeSH
- Case-Control Studies MeSH
- Age Distribution MeSH
- BK Virus immunology MeSH
- JC Virus immunology MeSH
- Animals MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- MeSH
- Killer Cells, Natural immunology MeSH
- Cytotoxicity, Immunologic MeSH
- T-Lymphocytes, Cytotoxic immunology MeSH
- Sarcoma, Experimental immunology blood MeSH
- Research Support as Topic MeSH
- Rats, Inbred Strains MeSH
- Neoplasm Invasiveness immunology ultrastructure MeSH
- Disease Models, Animal MeSH
- Tumor Cells, Cultured MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Keywords
- Manhattan, Dermacol, Constance Carrol,
- MeSH
- Data Interpretation, Statistical MeSH
- Cosmetics MeSH
- Rheology methods MeSH
