Q58529536
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Autologous cell therapy (ACT) is a new therapeutic approach for diabetic patients with no-option chronic limb-threatening ischemia (NO-CLTI). The aim of our study was to quantify cell populations of cell therapy products (CTPs) obtained by three different isolation methods and to correlate their numbers with changes in transcutaneous oxygen pressure (TcPO2). CTPs were separated either from stimulated peripheral blood (PB) (n = 11) or harvested from bone marrow (BM) processed either by Harvest SmartPReP2 (n = 50) or sedimented with succinate gelatin (n = 29). The clinical effect was evaluated by the change in TcPO2 after 1, 3 and 6 months. TcPO2 increased significantly in all three methods at each time point in comparison with baseline values (p < .01) with no significant difference among them. There was no correlation between the change in TcPO2 and the size of injected cell populations. We only observed a weak correlation between the number of injected white blood cells (WBC) and an increase in TcPO2 at 1 and 3 months. Our study showed that all three isolation methods of ACT were similarly relatively efficient in the treatment of NO-CLTI. We observed no correlation of TcPO2 increase with the number of injected monocytes, lymphocytes or CD34+. We observed a weak correlation between TcPO2 increase and the number of injected WBCs.
- MeSH
- autologní transplantace metody MeSH
- buněčná a tkáňová terapie * metody MeSH
- chronická kritická ischemie končetin * terapie diagnóza chirurgie MeSH
- ischemie * terapie etiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- transkutánní měření krevních plynů metody MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Stále rostoucí počet diabetiků v naší populaci klade vysoké nároky na screeningová vyšetření očního pozadí ve všech oftalmologických zařízeních. Tento problém se vyskytoval i v oční ambulanci centra diabetologie IKEM. Pro zjednodušení, urychlení a zpřesnění vyšetření očního pozadí u nemocných diabetem byl vytvořen jednoduchý uživatelsky příjemný program propojený s digitální kamerou a plně kompatibilní s lékařským informačním systémem IKEM (ZLATOKOP).
A growing number of diabetics in our population pose high demands on the screening of the fundus in all ophthalmologic departments. This problem also occurred in the ophthalmological outpatient department IKEM. A simple friendly-use software connected to the digital camera and fully compatible with the medical information system called Zlatokop (Prospector) has been created in IKEM. Its main purpose is to simplify, accelerate and clarify the examination of the fundus in diabetic patients.
- Klíčová slova
- VISUPACE, DICOM-PACS,
- MeSH
- audiovizuální záznam MeSH
- diabetická retinopatie * diagnóza prevence a kontrola MeSH
- fotografování * MeSH
- komplikace diabetu MeSH
- lidé MeSH
- nemocniční informační systémy * využití MeSH
- počítačové zpracování obrazu MeSH
- retina patologie MeSH
- software MeSH
- ukládání a vyhledávání informací metody MeSH
- Check Tag
- lidé MeSH
AIMS: The aim of this study was to compare the effects of calorie-restricted vegetarian and conventional diabetic diets alone and in combination with exercise on insulin resistance, visceral fat and oxidative stress markers in subjects with Type 2 diabetes. METHODS: A 24-week, randomized, open, parallel design was used. Seventy-four patients with Type 2 diabetes were randomly assigned to either the experimental group (n = 37), which received a vegetarian diet, or the control group (n = 37), which received a conventional diabetic diet. Both diets were isocaloric, calorie restricted (-500 kcal/day). All meals during the study were provided. The second 12 weeks of the diet were combined with aerobic exercise. Participants were examined at baseline, 12 weeks and 24 weeks. Primary outcomes were: insulin sensitivity measured by hyperinsulinaemic isoglycaemic clamp; volume of visceral and subcutaneous fat measured by magnetic resonance imaging; and oxidative stress measured by thiobarbituric acid reactive substances. Analyses were by intention to treat. RESULTS: Forty-three per cent of participants in the experimental group and 5% of participants in the control group reduced diabetes medication (P < 0.001). Body weight decreased more in the experimental group than in the control group [-6.2 kg (95% CI -6.6 to -5.3) vs. -3.2 kg (95% CI -3.7 to -2.5); interaction group × time P = 0.001]. An increase in insulin sensitivity was significantly greater in the experimental group than in the control group [30% (95% CI 24.5-39) vs. 20% (95% CI 14-25), P = 0.04]. A reduction in both visceral and subcutaneous fat was greater in the experimental group than in the control group (P = 0.007 and P = 0.02, respectively). Plasma adiponectin increased (P = 0.02) and leptin decreased (P = 0.02) in the experimental group, with no change in the control group. Vitamin C, superoxide dismutase and reduced glutathione increased in the experimental group (P = 0.002, P < 0.001 and P = 0.02, respectively). Differences between groups were greater after the addition of exercise training. Changes in insulin sensitivity and enzymatic oxidative stress markers correlated with changes in visceral fat. CONCLUSIONS: A calorie-restricted vegetarian diet had greater capacity to improve insulin sensitivity compared with a conventional diabetic diet over 24 weeks. The greater loss of visceral fat and improvements in plasma concentrations of adipokines and oxidative stress markers with this diet may be responsible for the reduction of insulin resistance. The addition of exercise training further augmented the improved outcomes with the vegetarian diet.
- MeSH
- biologické markery metabolismus MeSH
- diabetes mellitus 2. typu krev dietoterapie MeSH
- dieta vegetariánská MeSH
- dospělí MeSH
- glykovaný hemoglobin metabolismus MeSH
- inzulinová rezistence fyziologie MeSH
- krevní glukóza metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- oxidační stres fyziologie MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie MeSH
Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Promyelocytic leukemia nuclear bodies (PML NBs), the structural domains of the eukaryotic cell nucleus, play a role in cancer and apoptosis, and their involvement in antiviral mechanisms mediated by interferons (IFNs) is proposed. IFNs dramatically increase the transcription of the PML gene. In this study, we have shown that the response of 2 structural PML NB components, PML and Sp100, to interferon-alpha (IFNalpha) was suppressed in cells simultaneously treated with histone deacetylase (HDAC) inhibitors (trichostatin A, sodium butyrate, MS-275, SAHA, and valproic acid). Trichostatin A (TSA) blocked the increase of PML NB number and suppressed up-regulation of PML mRNA and protein levels in several human cell lines and in normal diploid skin fibroblasts. Moreover, IFNalpha induction of IRF-1 was also inhibited by TSA, although incompletely. Promyelocytic leukemia nuclear bodies (PML NBs), the structural domains of the eukaryotic cell nucleus, play a role in cancer and apoptosis, and their involvement in antiviral mechanisms mediated by interferons (IFNs) is proposed. IFNs dramatically increase the transcription of the PML gene. In this study, we have shown that the response of 2 structural PML NB components, PML and Sp100, to interferon-alpha (IFNalpha) was suppressed in cells simultaneously treated with histone deacetylase (HDAC) inhibitors (trichostatin A, sodium butyrate, MS-275, SAHA, and valproic acid). Trichostatin A (TSA) blocked the increase of PML NB number and suppressed up-regulation of PML mRNA and protein levels in several human cell lines and in normal diploid skin fibroblasts. Moreover, IFNalpha induction of IRF-1 was also inhibited by TSA, although incompletely.
- MeSH
- aktivace transkripce účinky léků MeSH
- financování organizované MeSH
- histondeacetylasy MeSH
- inhibitory enzymů farmakologie MeSH
- inhibitory histondeacetylas MeSH
- interferon alfa farmakologie MeSH
- jaderné proteiny genetika MeSH
- kultivované buňky MeSH
- lidé MeSH
- nádorové buňky kultivované MeSH
- nádorové proteiny genetika MeSH
- nádorové supresorové proteiny genetika MeSH
- RNA nádorová analýza MeSH
- transkripční faktor STAT2 metabolismus MeSH
- transkripční faktory genetika MeSH
- upregulace účinky léků MeSH
- Check Tag
- lidé MeSH
Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.
- MeSH
- buněčné jádro metabolismus MeSH
- buněčné linie MeSH
- exprese genu MeSH
- financování organizované MeSH
- fylogeneze MeSH
- genetická transkripce MeSH
- konzervovaná sekvence MeSH
- lidé MeSH
- myosin typu I genetika chemie izolace a purifikace metabolismus MeSH
- myši MeSH
- obratlovci genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie nukleových kyselin MeSH
- sérum chemie MeSH
- techniky amplifikace nukleových kyselin MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
