Q59674433
Dotaz
Zobrazit nápovědu
Omentin is a protein produced by numerous tissues including adipose tissue. Its concentrations are decreased in patients with obesity, type 2 diabetes mellitus (DM) and coronary artery disease (CAD). Experimental studies suggest that omentin may have anti-inflammatory and insulin-sensitizing properties. In the present study, we measured circulating omentin levels and its mRNA expression in epicardial and subcutaneous fat, intercostal and heart muscle before and after elective cardiac surgery in patients with CAD (CAD+, DM-, n=18), combination of CAD and DM (CAD+, DM+, n=9) or with none of these conditions (CAD-, DM-, n=11). The groups did not differ in baseline anthropometric and biochemical characteristics with the exception of higher blood glucose and HBA(1c) in CAD+, DM+ group. Baseline circulating omentin levels tended to be lower in CAD+, DM- and CAD+, DM+ groups as compared to CAD-, DM- group and cardiac surgery increased its concentration only in CAD-, DM- group. The change in serum omentin levels during surgery inversely correlated with epicardial fat thickness. While baseline omentin mRNA expression did not differ among the groups in any of the studied tissues, its increase after surgery was present only in subcutaneous fat in CAD-, DM- and CAD+, DM- groups, but not in CAD+, DM+ group. Intercostal muscle omentin mRNA expression increased after surgery only in CAD-, DM- group. In conclusion, cardiac surgery differentially affects omentin levels and subcutaneous fat and skeletal muscle mRNA expression in patients without coronary artery disease and diabetes as compared to patients with these conditions.
- MeSH
- biologické markery krev MeSH
- cytokiny krev genetika MeSH
- diabetes mellitus 2. typu krev chirurgie MeSH
- dospělí MeSH
- elektivní chirurgické výkony * MeSH
- exprese genu MeSH
- GPI-vázané proteiny krev genetika MeSH
- lektiny krev genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA krev genetika MeSH
- nemoci koronárních tepen krev chirurgie MeSH
- perikard metabolismus MeSH
- senioři MeSH
- tuková tkáň metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- chronická obstrukční plicní nemoc * diagnostické zobrazování chirurgie rehabilitace MeSH
- dechová cvičení metody přístrojové vybavení MeSH
- dýchací svaly MeSH
- dýchání MeSH
- dyspnoe rehabilitace MeSH
- lidé MeSH
- mechanika dýchání MeSH
- plicní emfyzém rehabilitace MeSH
- pneumektomie metody MeSH
- Check Tag
- lidé MeSH
Mitochondrial dysfunction is a potentially important player in the development of insulin resistance and type 2 diabetes mellitus (T2DM). We investigated the changes of mRNA expression of genes encoding main enzymatic complexes of mitochondrial respiratory chain in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 11 subjects with simple obesity (OB), 16 obese patients with T2DM and 17 healthy lean subjects (C) before and after very low-calorie diet (VLCD) using quantitative real time PCR. At baseline in SCAT, both T2DM and OB group had decreased mRNA expression of all investigated mitochondrial genes with the exception of 2 complex I (NDUFA 12) and complex IV (COX 4/1) enzymes in OB subjects. In contrast, in PM only the expression of complex I enzymes NDUFA 12 and MT-ND5 was reduced in both T2DM and OB subjects along with decreased expression of citrate synthase (CS) in T2DM group. Additionally, T2DM subjects showed reduced activity of pyruvate dehydrogenase and complex IV in peripheral blood elements. VLCD further decreased mRNA expression of CS and complex I (NT-ND5) and II (SDHA) enzymes in SCAT and complex IV (COX4/1) and ATP synthase in PM of T2DM group, while increasing the activity of complex IV in their peripheral blood elements. We conclude that impaired mitochondrial biogenesis and decreased activity of respiratory chain enzymatic complexes was present in SCAT and PM of obese and diabetic patients. VLCD improved metabolic parameters and ameliorated mitochondrial oxidative function in peripheral blood elements of T2DM subjects but had only minor and inconsistent effect on mitochondrial gene mRNA expression in SCAT and PM.
- MeSH
- diabetes mellitus 2. typu krev dietoterapie epidemiologie MeSH
- dospělí MeSH
- kalorická restrikce metody trendy MeSH
- leukocyty mononukleární metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mitochondrie metabolismus MeSH
- obezita krev dietoterapie epidemiologie MeSH
- podkožní tuk metabolismus MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
AIM OF THE STUDY: Angiopoietin-like protein 6 (ANGPTL6) is a circulating protein with a potential role in energy homeostasis. The aim of the study was to explore the changes in ANGPTL6 levels in patients with obesity (Body mass index, BMI > 40 kg/m(2)) with and without type 2 diabetes mellitus (T2DM) undergoing dietary intervention (very low calorie diet - VLCD) and in a subgroup of T2DM patients after bariatric surgery. Additionally, we examined changes in ANGPTL6 in anorexia nervosa (AN) patients at baseline and after partial realimentation. We also explored the changes in ANGPTL6 mRNA expression in subcutaneous adipose tissue (SAT) of obese subjects. MATERIALS AND METHODS: The study included 23 non-diabetic obese patients, 40 obese patients with T2DM (27 underwent VLCD and 13 underwent bariatric surgery), 22 patients with AN, and 37 healthy control subjects. RESULTS: ANGPTL6 levels of AN patients were increased relative to the control group (68.6 ± 9.9 ng/ml) and decreased from 110.2 ± 13.3 to 73.6 ± 7.1 ng/ml (p = 0.004) after partial realimentation. Baseline ANGPTL6 levels in patients with obesity and T2DM did not differ from the control group. VLCD decreased ANGPTL6 levels only in obese patients with T2DM. Bariatric surgery induced a transient elevation of ANGPTL6 levels with a subsequent decrease to baseline levels. ANGPTL6 mRNA expression transiently increased after bariatric surgery and returned to baseline levels after 12 months. CONCLUSIONS: Collectively, our data suggest that serum ANGPTL6 levels and ANGPTL6 mRNA expression in SAT are affected by metabolic disorders and their treatment but do not appear to directly reflect nutritional status.
- MeSH
- angiopoetiny krev MeSH
- bariatrická chirurgie MeSH
- diabetes mellitus 2. typu krev dietoterapie chirurgie MeSH
- kalorická restrikce MeSH
- lidé středního věku MeSH
- lidé MeSH
- mentální anorexie krev dietoterapie metabolismus MeSH
- obezita krev dietoterapie chirurgie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Počet incidence chronické obstrukční plicní nemoci ve světě narůstá. Tato diagnóza poutá pozornost odborné sféry především pro zdravotní důsledky a vyplývající ekonomickou zátěž. Na základě rozšiřování poznatků a vyspívání medicíny se ve značné míře klasická chirurgie zaměřuje na miniinvazivní výkony. V problematice emfyzému se výkony význačně přesouvají od chirurgického k endoskopickému zákroku. Četné množství studií dokládá pozitivní efekt tohoto typu výkonu nejen na funkční parametry plic, ale i na úroveň fyzické výkonnosti. Důležitou oblastí komplexního přístupu v léčbě je možnost podpory výkonem dosaženého výsledného profilu v podobě fyzioterapie u pacientů, kterým je tato forma výkonu indikována.
The incidence of the chronic obstructive pulmonary disease is increasing all over the world. Experts are interested in this diagnosis especially for its effects on health and negative economic outcomes. Due to expanding knowledge in medicine the classical surgery is to a large extend focused on mini invasive procedures. In the field of emphysema, the procedures are to a considerable degree shifting from surgical to endoscopic treatment. Many recent studies document its positive impact not only on functional parameters of the lungs, but also on the level of physical performance. An important area in complex therapy is the possibility of supporting the final profile reached by this procedure with the physiotherapy.
- Klíčová slova
- bronchoskopická volumredukce,
- MeSH
- bronchoskopie metody MeSH
- chronická obstrukční plicní nemoc * chirurgie MeSH
- lidé MeSH
- plicní emfyzém chirurgie MeSH
- pneumektomie * metody MeSH
- Check Tag
- lidé MeSH
Clusterin is a heterodimeric glycoprotein with wide range of functions. To further explore its possible regulatory role in energy homeostasis and in adipose tissue, we measured plasma clusterin and its mRNA expression in subcutaneous adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired glucose tolerance (IGT) and 8 normoglycemic patients who underwent hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA expression in SCAT at baseline was increased in OB and T2DM patients compared with controls. Clusterin mRNA expression decreased 6 months after LSG and remained decreased 12 months after LSG. mRNA expression of clusterin was elevated at the end of HEC compared with baseline only in normoglycemic but not in IGT or T2DM patients. In summary, our data suggest a possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis.
- MeSH
- bariatrická chirurgie * MeSH
- čipová analýza proteinů MeSH
- diabetes mellitus 2. typu krev komplikace MeSH
- energetický metabolismus MeSH
- kalorická restrikce * MeSH
- klusterin krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA metabolismus MeSH
- obezita krev komplikace terapie MeSH
- podkožní tuk metabolismus MeSH
- studie případů a kontrol MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
We explored the effect of chronically elevated circulating levels of growth hormone (GH)/insulin-like-growth-factor-1 (IGF-1) on mRNA expression of GH/IGF-1/insulin axis components and p85alpha subunit of phosphoinositide-3-kinase (p85alpha) in subcutaneous adipose tissue (SCAT) of patients with active acromegaly and compared these findings with healthy control subjects in order to find its possible relationships with insulin resistance and body composition changes. Acromegaly group had significantly decreased percentage of truncal and whole body fat and increased homeostasis model assessment-insulin resistance (HOMA-IR). In SCAT, patients with acromegaly had significantly increased IGF-1 and IGF-binding protein-3 (IGFBP-3) expression that both positively correlated with serum GH. P85alpha expression in SCAT did not differ from control group. IGF-1 and IGFBP-3 expression in SCAT were not independently associated with percentage of truncal and whole body fat or with HOMA-IR while IGFBP-3 expression in SCAT was an independent predictor of insulin receptor as well as of p85alpha expression in SCAT. Our data suggest that GH overproduction in acromegaly group increases IGF-1 and IGFBP-3 expression in SCAT while it does not affect SCAT p85alpha expression. Increased IGF-1 or IGFBP-3 in SCAT of acromegaly group do not appear to contribute to systemic differences in insulin sensitivity but may have local regulatory effects in SCAT of patients with acromegaly.
- MeSH
- akromegalie metabolismus MeSH
- dospělí MeSH
- fosfatidylinositol-3-kinasy třídy Ia krev MeSH
- IGFBP-3 metabolismus MeSH
- insulinu podobný růstový faktor I metabolismus MeSH
- inzulin krev MeSH
- inzulinová rezistence * MeSH
- lidé středního věku MeSH
- lidé MeSH
- lidský růstový hormon krev sekrece MeSH
- messenger RNA metabolismus MeSH
- podkožní tuk metabolismus MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
