Takac, Peter* Dotaz Zobrazit nápovědu
OBJECTIVE: To investigate the antibacterial properties of lucifensin in maggots of Lucilia sericata after septic injury. METHODS: In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands. It is assumed that lucifensin is secreted solely from this tissue into the haemolymph (similar to other insect defensins) and not into secreted/excreted products. We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemolymph extracted from larvae after septic injury. RESULTS: After septic injury, production of lucifensin in the haemolymph is increased. This led to higher antibacterial activity of such haemolymph in comparison to non-stimulated larvae. COCLUSIONS: These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy, lucifensin is simultaneously important as a part of the systematic immune response.
- Publikační typ
- časopisecké články MeSH
x
- MeSH
- aspartátové proteasy genetika sekrece izolace a purifikace MeSH
- debridement * metody MeSH
- Diptera MeSH
- genová knihovna MeSH
- helmintoterapie MeSH
- hojení ran MeSH
- hybridizace in situ MeSH
- komplementární DNA genetika MeSH
- larva * enzymologie genetika růst a vývoj MeSH
- lidé MeSH
- metaloproteasy genetika izolace a purifikace sekrece MeSH
- nekróza terapie MeSH
- proteasy * genetika izolace a purifikace sekrece MeSH
- sekvenční analýza DNA MeSH
- serinové proteasy genetika sekrece izolace a purifikace MeSH
- slinné žlázy enzymologie MeSH
- trávicí systém enzymologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Techniques to quantify postural stability usually rely on the evaluation of only two variables, that is, two coordinates of COP. However, by using three variables, that is, three components of acceleration vector, it is possible to describe human movement more precisely. For this purpose, a single three-axis accelerometer was used, making it possible to evaluate 3D movement by use of a novel method, convex polyhedron (CP), together with a traditional method, based on area of the confidence ellipse (ACE). Ten patients (Pts) with cerebellar ataxia and eleven healthy individuals of control group (CG) participated in the study. The results show a significant increase of volume of the CP (CPV) in Pts or CG standing on foam surface with eyes open (EO) and eyes closed (EC) after the EC phase. Significant difference between Pts and CG was found in all cases as well. Correlation coefficient indicates strong correlation between the CPV and ACE in most cases of patient examinations, thus confirming the possibility of quantification of postural instability by the introduced method of CPV.
- MeSH
- akcelerometrie metody MeSH
- dospělí MeSH
- lidé MeSH
- postura těla fyziologie MeSH
- posturální rovnováha fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
1.vyd. 219 s.
- Klíčová slova
- texty učební, propedeutika,
- MeSH
- rehabilitace MeSH
1. elektronické vydání 1 online zdroj (264 stran)
Kniha je určená pre študentov a odborníkov primárne z radov farmaceutov, laborantov. Nevysvetľuje základné mechanizmy a princípy účinku jednotlivých liečiv, ale poskytuje interaktívny pohľad na racionálnu farmakoterapiu v podobe inšpiratívnych informácií, praktických cvičení, modelových situácii a iných zaujímavých úloh.
- Klíčová slova
- rehabilitační léčba,
- MeSH
- biofeedback (psychologie) MeSH
- inkontinence moči MeSH
- pánevní dno MeSH
