Autoři předkládají své patnáctiměsíční zkušenosti při terapii mužské infertility mužů s posttraumatickou fa-ansverzální míšní lézí. K realizaci biologického otcovství takto handicapovaných mužů, u kterých je velmi častým nálezem anejakulače, používají elektroejakulační rektální sondu, pomocí níž vybavili ejakulát u 100 % vyšetřovaných mužů. Spermiologické zhodnocení s nálezem astenospermie je limitujícím pro výběr vhodné metody asistované reprodukce. Spolupráce s cenfrem asistované reprodukce, které vede hormonální pnpravu ženy k asistované reprodukci a spolupráce s psycholožkou dotváří komplexní, mezioborovou péči o infertilní, paraplegický pár.

The authors submit their 15-month experience with the freatinent of infertility in men with postraumatic transverse spinal lesions. For implementation of biological paternity of thus handicapped men, where anejaculation is frequent the authors use a rectal electroejaculation tube by means of which they produced an ejaculate in 100 % of the examined men. Spermiological evaluation with the finding of asthenospermia is limiting for selection of a suitable method of assisted reproduction. Collaboration with the centre of assisted reproduction which ensures hormonal preparation of women for assisted reproduction and collaboration with a psychologist are part of the comprehensive interdisciplinary care of infertile, paraplegic couples.

• MeSH
• dospělí MeSH
• ejakulace MeSH
• lidé MeSH
• mužská infertilita terapie   MeSH
• paraplegie MeSH
• úrazy a nehody MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH

• MeSH
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• nádorové biomarkery MeSH
• testikulární nádory MeSH
• urologické chirurgické výkony u mužů MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• nefrologie
• onkologie
• urologie

The aim of our study was to compare the quality parameters of fresh feline ejaculates collected by three different techniques-urethral catheterization after medetomidine administration (CT), electroejaculation (EE) and epididymal slicing after orchiectomy (EP). A total of 34 adult male cats (Felis catus) were included in the study. In all male cats, the sperm collection was performed under general anaesthesia by three collection methods in the following order: urethral catheterization, electroejaculation and epididymal slicing. The sperm parameters evaluated were as follows: volume, motility, viability, sperm concentration, total sperm count and morphological examination. The highest quality semen parameters were achieved using EE. The comparison of results of the evaluated sperm quality parameters from EE and EP showed significant differences only in one case-the percentage of head abnormalities and lower percentage of head abnormalities were achieved using EE compared to EP: 8.5% (3.0%-21.0%) versus 10.0% (4.0%-22.0%). Semen collected by CT rendered the lowest quality samples when compared to sperm samples collected by EE and EP, especially with respect to the motility and total sperm count which were significantly lower (p < 0.001). Our study showed that sperm samples collected by EE and EP result in better quality of feline ejaculates compared to collection by CT from sperm samples collected from the same male cats. These results demonstrate the necessity of further research of urethral catheterization as a novel technique of semen collection in male cats.

• MeSH
• analýza spermatu veterinární   MeSH
• ejakulace * MeSH
• elektrická stimulace * MeSH
• katetrizace močového měchýře veterinární   MeSH
• kočky MeSH
• kryoprezervace veterinární   MeSH
• medetomidin farmakologie   MeSH
• odběr biologického vzorku veterinární   MeSH
• orchiektomie veterinární   MeSH
• uchování spermatu veterinární   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Ansell's mole-rats (Fukomys anselli) are subterranean rodents living in families composed of about 20 members with a single breeding pair and their non-breeding offspring. Most of them remain with their parents for their lifetime and help to maintain and defend the natal burrow system, forage, and care for younger siblings. Since incest avoidance is based on individual recognition (and not on social suppression) we expect that non-breeders produce viable sperm spontaneously. We compared the sperm of breeding and non-breeding males, obtained by electroejaculation and found no significant differences in sperm parameters between both groups. Here, we used electroejaculation to obtain semen for the first time in a subterranean mammal. Spermiogram analysis revealed no significant differences in sperm parameters between breeders and non-breeders. We found significantly larger testes (measured on autopsies and on living animals per ultrasonography) of breeders compared to non-breeders (with body mass having a significant effect). There were no marked histological differences between breeding and non-breeding males, and the relative area occupied by Leydig cells and seminiferous tubules on histological sections, respectively, was not significantly different between both groups. The seminiferous epithelium and to a lesser degree the interstitial testicular tissue are characterized by lesions (vacuolar degenerations), however, this feature does not hinder fertilization even in advanced stages of life. The continuous production of viable sperm also in sexually abstinent non-breeders might be best understood in light of the mating and social system of Fukomys anselli, and the potential to found a new family following an unpredictable and rare encounter with an unfamiliar female ("provoked or induced dispersal"). Apparently, the non-breeders do not reproduce because they do not copulate but not because they would be physiologically infertile. The significantly increased testes volume of breeding males (compared to non-breeders) is in agreement with previously found higher testosterone levels of breeders.

• MeSH
• chov metody   MeSH
• mikroftalmičtí podzemní hlodavci fyziologie   MeSH
• rozmnožování fyziologie   MeSH
• spermie fyziologie   MeSH
• testis fyziologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Sexuální dysfunkce je častým následkem poranění míchy. Erektilní dysfunkce a anejakulace jsou běžným důsledkem spinálního traumatu. Řešením je užití perorální léčby inhibitory fosfodiesterázy 5 (PDE-5) či intrakavernózní injekce prostaglandinu E1. Spermie je možno získat pomocí elektroejakulace nebo TESE (extrakce spermií z varlat). Metodou asistované reprodukce ICSI (intracytoplazmatická injekce spermie) realizujeme biologické otcovství ochrnutých mužů. Sexuolog-androlog je členem terapeutického týmu Spinální jednotky Úrazové nemocnice v Brně. Prvním krokem je podání informace o možném vzniku sexuální dysfunkce v souvislosti s poraněním míchy. Ve druhé fázi řešíme problematiku erektilní dysfunkce, infertility a ženské sexuální dysfunkce.

Sexual dysfunction is a frequent problem after spinal cord injury. Erectile dysfunction and anejaculation are a common consequence spinal cord injury. In the treatment of erectile dysfunction is the oral treatment of PDE-5 inhibitors or intracavernous injections of PGE1. The possibilities to obtain semen are electroejaculation or TESE (Testicular Sperm Extraction). By assisted reproductive technique by intracytoplasmic sperm injection we obtain biological paternity of paraplegic men. A sexologist-andrologist is a member of the therapeutic team of the Spinal Unit of the Traumatological Hospital Brno. In the first stage a sexologist informs the spinal cord patients about possible sexual dysfunction. In the second stage we solve particular problems of the erectile dysfunction, infertility and female sexual dysfunction.

• MeSH
• asistovaná reprodukce využití   MeSH
• erektilní dysfunkce diagnóza   etiologie   terapie   MeSH
• inhibitory fosfodiesteras aplikace a dávkování   terapeutické užití   MeSH
• karboliny aplikace a dávkování   terapeutické užití   MeSH
• lidé MeSH
• poranění míchy epidemiologie   komplikace   terapie   MeSH
• prostaglandiny aplikace a dávkování   terapeutické užití   MeSH
• sexuální dysfunkce fyziologická diagnóza   etiologie   terapie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH
• přehledy MeSH

Sclerosis multiplex (SM) je demyelinizujúce ochorenie neznámej etiológie, ktoré postihuje centrálny nervový systém – mozog a miechu. Fertilita u každého mužského neurologického pacienta môže byť negatívne ovplyvnená erektilnou dysfunkciou (ED), poruchami ejakulácie, poruchou kvality spermií alebo rôznymi kombináciami týchto troch porúch. ED sa dá zvládnuť liečebnými metódami rôznej invazivity vrátane liekov, injekčnej terapie a implantáciou penilných protéz. Retrográdna ejakulácia je riešiteľná farmakoterapiou v miernych prípadoch a „zberom“ spermií z močového mechúra po ejakulácii v závažnejších prípadoch. Anejakuláci môže byť manažovaná medikamentózne v miernych prípadoch, zatiaľ čo asistované ejakulačné techniky vrátane penilnej vibračnej stimulácie a elektrojakulácie sa používajú v závažnejších prípadoch. Ak asistovaná ejakulácia zlyhá, spermie je možné získať chirurgicky buď z nadsemenníka, alebo zo semenníka. Pri obdržaní životaschopných spermií, sú využiteľné techniky asistovanej reprodukcie vrátane intravaginálnej inseminácie, intrauterinnej inseminácie a in vitro fertilizácie/ intracytoplazmatickej injekcie spermií. Na zhodnotenie fertility a kvality spermy u mužov so SM zatiaľ prebehol len malý výskum, a preto sú potrebné ďalšie klinické štúdie a užšia medziodborová spolupráca.

Multiple sclerosis (MS) is a demyelinating disease of unknown etiology that affects the central nervous system – both the brain andthe spinal cord. Male fertility can be compromised in the neurological patient by erectile dysfunction (ED), ejaculation disorder,impaired sperm quality or various combinations of these three disorders. ED can be managed by an increasingly invasive rangeof treatments including medications, injection therapy and the surgical insertion of a penile implant. Retrograde ejaculation ismanaged by medications to reverse the condition in mild cases and in bladder harvest of semen after ejaculation in more severecases. Anejaculation might also be managed by medication in mild cases while assisted ejaculatory techniques including penilevibratory stimulation and electroejaculation are used in more severe cases. In cases where assisted ejaculation fails, sperm canbe retrieved surgically from either the epididymis or from the testis. Once viable sperm cells have been obtained, there are usedin assisted reproductive techniques, including intravaginal insemination, intrauterine insemination, and in vitro fertilization/intracytoplasmic sperm injection. Only small research has been performed to evaluate fertility and sperm quality in SM men, sofurther clinical studies and close interdisciplinary collaboration are needed.

• MeSH
• alprostadil terapeutické užití   MeSH
• analýza spermatu metody   MeSH
• asistovaná reprodukce MeSH
• erektilní dysfunkce * farmakoterapie   terapie   MeSH
• inhibitory fosfodiesterasy 5 terapeutické užití   MeSH
• lidé MeSH
• mužská infertilita * diagnóza   farmakoterapie   terapie   MeSH
• odběr spermií MeSH
• penilní protézy MeSH
• roztroušená skleróza * diagnóza   komplikace   terapie   MeSH
• sexuální dysfunkce fyziologická * terapie   MeSH
• vazokonstriktory terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH

Development of autonomic dysreflexia (AD) is described in patients with high transversal spinal lesion – above the level C6. While applying methods of assisted reproduction, AD is described at performing artificial ejaculation – vibrostimulation or electroejaculation. We have not found any references to occurence of AD in connection to TESE in the Medline database. Material and methods: A 32-year-old patient, 16 years after injury with a spinal lesion at the level C6, was admitted to have TESE performed under spinal anesthesia. The procedure was without complications. A permanent urinary catheter was introduced, which the patient had no problem torelating in the past. Once the effects of anesthesia subsided, autonomic dysreflexia developed – anxiety and restlessness, profuse sweating on the upper half of the body with gradual increase in blood pressure up to values of 220/130, massive macroscopic hematuria appears. Upon introduction of complex pharmacotherapy regimen, there is only partial stabilization of the condition. Final normalization of the condition occurs 60 hours after extraction of the permanent urinary catheter, which had been left there before due to danger of tamponade of the urinary bladder. Further course was without complications. Results: Overall high reflex activity in a patient with a high spinal lesion, along with a long-term poor bladder emptying and a surgical procedure in an exceptionally strong reflexively active area, lead to the development of AD, which was managed only after removing all offending stimuli from the pelvic area and after consistent complex pharmacotherapy, i.e. after consistent administration of analgesics, anxiolytics and a combination of antihypertensitives. Conclusions: Performing TESE on patients with high spinal lesion can be accompanied by AD not only directly during the surgical procedure, but also in postoperative period.

• Publikační typ
• abstrakty MeSH

The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon® PMSG Serum Gonadotrophin) on day 12, upon CIDR removal. They were then inseminated with one of each sperm treatment, at the following post-eCG intervals: I_1, 55:01-55:30 h; I_2, 55:31-56:00 h; I_3, 56:01-56:30 h; or, I_4, 56:31-57:00 h. Pregnancy rates were assessed at parturition. Average pregnancy rates were highest (P < 0.05) for Conventional samples (77.6%), but similar between YSS (49.8%) and Control sorted (51.3%) samples. However, when insemination interval was taken into account, pregnancy rates within the YSS group, pregnancy rates were 80 and 83.1% for I_1 and I_2, respectively were obtained. Notably, these rates were similar (P > 0.05) to the average pregnancy rates obtained with Conventional samples (77.6%). As expected, YSS sperm yielded 94% male offspring contrasting with the 57% males obtained with Conventional and Control sorted samples. Our findings support the importance of developing specific insemination timing protocols to improve pregnancy rates when using frozen-thawed sex-sorted sperm. These findings provide the foundation for further investigations in order to determine why the YSS sperm are able to fertilize the oocyte in a shorter period of time than the conventional samples.

• MeSH
• fertilita fyziologie   MeSH
• kryoprezervace veterinární   MeSH
• předvýběr pohlaví veterinární   MeSH
• spermie fyziologie   MeSH
• těhotenství MeSH
• uchování spermatu veterinární   MeSH
• umělá inseminace veterinární   MeSH
• vysoká zvěř fyziologie   MeSH
• zmrazování * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen. Two straws per stag and sample type were analyzed immediately post-thaw and following a 2-h incubation period at 37 °C. Post-thaw total motility (TM) as assessed by CASA was not different (P < 0.05) among NS, BSS and YSS sperm. For XSS, post-thaw TM was lower (39%, P < 0.05) than that for NS (54%) or BSS (50%), but similar (P > 0.05) to that of YSS (47%) sperm. The percentage of apoptotic spermatozoa as assessed by PI/YO-PRO-1 and flow cytometry analysis, was higher (17%, P ≤ 0.05) for XSS sperm than NS (12%), BSS (13%) and YSS (14%) sperm. Following incubation there were no differences (P > 0.05) in TM or percent apoptosis among treatments. Post-thaw chromatin stability calculated as the DNA fragmentation index (%DFI) was similar among treatments; following incubation %DFI increased in all except YSS, which displayed the lowest value (P < 0.05). Artificial insemination of synchronized hinds yielded 44, 52 and 62% delivery rates for YSS, NS and standard frozen-thawed sperm, respectively (P < 0.05). Notably, 93 and 55% of fawns born were males for the YSS and NS spermatozoa, respectively (P < 0.05). In summary, Y-sorted sperm displayed acceptable post-thaw sperm evaluation parameters and the expected offspring sex ratio. More studies are needed to understand the source of sperm damage that may compromise the fertility of Y-sorted red deer sperm.

• MeSH
• fragmentace DNA MeSH
• kryoprezervace metody   veterinární   MeSH
• motilita spermií MeSH
• poměr pohlaví MeSH
• předvýběr pohlaví veterinární   MeSH
• průtoková cytometrie veterinární   MeSH
• spermie fyziologie   MeSH
• uchování spermatu metody   veterinární   MeSH
• umělá inseminace veterinární   MeSH
• vysoká zvěř * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• infertilita MeSH
• reprodukční techniky MeSH
• rozmnožování MeSH
• Publikační typ
• abstrakty MeSH
• kongresy MeSH
• sborníky MeSH

• Konspekt
• Gynekologie. Porodnictví
• NLK Obory
• reprodukční lékařství